Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract
Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on th...
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Universitas Indonesia
2019
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| Online Access: | http://irep.iium.edu.my/75057/ http://irep.iium.edu.my/75057/ http://irep.iium.edu.my/75057/1/Gene%20expression%20of%20selected%20apoptotic%20markers-aisyah%20et%20al.pdf |
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iium-750572019-10-30T07:25:58Z http://irep.iium.edu.my/75057/ Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract Lestari, Widya Wan Yusry, Wan N A Iskandar, Siti H. Ichwan, Solachuddin J. A. Irfan, Nining I. Suriyah, Wastuti Hidayati RK Dentistry Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on the expression of apoptotic markers, Bax and Bcl-2. Methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was dried thoroughly and then extracted aqueously. The apoptotic activity was detected via flow cytometry. Bax and Bcl-2 expression was analyzed by quantitative real-time polymerase chain reaction. Results: Results of our cell cycle analysis reveal that aqueous extract of M. pendans induced apoptosis in 2.5 and 5 mg/mL but no change between these two concentrations. Apoptosis was observed at 24 h but not at 48 h. Bax and Bcl-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, upregulation of Bax was observed at 2.5 mg/mL. However, after 48 h, Bax expression showed no changes at any concentration. Bcl-2 was significantly downregulated after 48 h of treatment. Conclusions: M. pendans extract induced apoptosis in HSC-3 cells, which might occur via the proapoptotic (Bax) and antiapoptotic (Bcl-2) pathways. Universitas Indonesia 2019-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/75057/1/Gene%20expression%20of%20selected%20apoptotic%20markers-aisyah%20et%20al.pdf Lestari, Widya and Wan Yusry, Wan N A and Iskandar, Siti H. and Ichwan, Solachuddin J. A. and Irfan, Nining I. and Suriyah, Wastuti Hidayati (2019) Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract. Makara Journal of Health Research, 23 (2). pp. 121-126. ISSN 2356-3664 E-ISSN 2356-3656 https://scholarhub.ui.ac.id/cgi/viewcontent.cgi?article=1009&context=mjhr |
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Digital Repository |
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Local University |
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Online Access |
| language |
English |
| topic |
RK Dentistry |
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RK Dentistry Lestari, Widya Wan Yusry, Wan N A Iskandar, Siti H. Ichwan, Solachuddin J. A. Irfan, Nining I. Suriyah, Wastuti Hidayati Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| description |
Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was
recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study
aimed to investigate the effect of M. pendans treated samples on the expression of apoptotic markers, Bax and Bcl-2.
Methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was dried thoroughly and then
extracted aqueously. The apoptotic activity was detected via flow cytometry. Bax and Bcl-2 expression was analyzed by
quantitative real-time polymerase chain reaction. Results: Results of our cell cycle analysis reveal that aqueous extract
of M. pendans induced apoptosis in 2.5 and 5 mg/mL but no change between these two concentrations. Apoptosis was
observed at 24 h but not at 48 h. Bax and Bcl-2 expression in HSC-3 cells was affected by M. pendans. At 24 h,
upregulation of Bax was observed at 2.5 mg/mL. However, after 48 h, Bax expression showed no changes at any
concentration. Bcl-2 was significantly downregulated after 48 h of treatment. Conclusions: M. pendans extract induced
apoptosis in HSC-3 cells, which might occur via the proapoptotic (Bax) and antiapoptotic (Bcl-2) pathways. |
| format |
Article |
| author |
Lestari, Widya Wan Yusry, Wan N A Iskandar, Siti H. Ichwan, Solachuddin J. A. Irfan, Nining I. Suriyah, Wastuti Hidayati |
| author_facet |
Lestari, Widya Wan Yusry, Wan N A Iskandar, Siti H. Ichwan, Solachuddin J. A. Irfan, Nining I. Suriyah, Wastuti Hidayati |
| author_sort |
Lestari, Widya |
| title |
Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_short |
Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_full |
Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_fullStr |
Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_full_unstemmed |
Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_sort |
gene expression of selected apoptotic markers in human oral squamous carcinoma hsc-3 cell line treated with myrmecodia pendans plant extract |
| publisher |
Universitas Indonesia |
| publishDate |
2019 |
| url |
http://irep.iium.edu.my/75057/ http://irep.iium.edu.my/75057/ http://irep.iium.edu.my/75057/1/Gene%20expression%20of%20selected%20apoptotic%20markers-aisyah%20et%20al.pdf |
| first_indexed |
2023-09-18T21:46:13Z |
| last_indexed |
2023-09-18T21:46:13Z |
| _version_ |
1777413467280506880 |