Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract
Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on th...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Indonesia
2019
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/75057/ http://irep.iium.edu.my/75057/ http://irep.iium.edu.my/75057/1/Gene%20expression%20of%20selected%20apoptotic%20markers-aisyah%20et%20al.pdf |
| Summary: | Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was
recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study
aimed to investigate the effect of M. pendans treated samples on the expression of apoptotic markers, Bax and Bcl-2.
Methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was dried thoroughly and then
extracted aqueously. The apoptotic activity was detected via flow cytometry. Bax and Bcl-2 expression was analyzed by
quantitative real-time polymerase chain reaction. Results: Results of our cell cycle analysis reveal that aqueous extract
of M. pendans induced apoptosis in 2.5 and 5 mg/mL but no change between these two concentrations. Apoptosis was
observed at 24 h but not at 48 h. Bax and Bcl-2 expression in HSC-3 cells was affected by M. pendans. At 24 h,
upregulation of Bax was observed at 2.5 mg/mL. However, after 48 h, Bax expression showed no changes at any
concentration. Bcl-2 was significantly downregulated after 48 h of treatment. Conclusions: M. pendans extract induced
apoptosis in HSC-3 cells, which might occur via the proapoptotic (Bax) and antiapoptotic (Bcl-2) pathways. |
|---|