Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology

Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, wh...

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Main Authors:	Mahmud, Israa Ali, Mirghani, Mohamed Elwathig Saeed, Yusof, Faridah, Al-Khatib, Ma'an Fahmi Rashid
Format:	Article
Language:	English
Published:	MDPI 2019
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf

id	iium-75015
recordtype	eprints
spelling	iium-750152019-11-08T07:03:45Z http://irep.iium.edu.my/75015/ Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid T Technology (General) Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro. MDPI 2019-07 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf Mahmud, Israa Ali and Mirghani, Mohamed Elwathig Saeed and Yusof, Faridah and Al-Khatib, Ma'an Fahmi Rashid (2019) Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology. Preprints. pp. 1-36. (Unpublished) https://www.preprints.org/manuscript/201907.0055/v1 https://www.preprints.org/manuscript/201907.0055/v1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
description	Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro.
format	Article
author	Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid
author_facet	Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid
author_sort	Mahmud, Israa Ali
title	Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_short	Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_full	Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_fullStr	Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_full_unstemmed	Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_sort	effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
publisher	MDPI
publishDate	2019
url	http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf
first_indexed	2023-09-18T21:46:10Z
last_indexed	2023-09-18T21:46:10Z
_version_	1777413464030969856

Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology

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