Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds

Background and objectives: The initiation of apoptotic death in cancer cells is the aim of many chemotherapeutic strategies. As many anticancer agents have been discovered in natural plant sources, the search for novel phytochemicals displaying anticancer activities continues at a tremendous rate. T...

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Main Authors:	Mahmud, Israa Ali, Mirghani, Mohamed Elwathig Saeed, Al-Qubaisi, Mothanna, , Beh Yee, Ismail, Norsharina, Rasedee, Abdullah, Yusof, Faridah, Shahabuddin, Munira, Al-khatib, Ma'an, Kabbashi, Nassereldeen Ahmed, Hashim, Yumi Zuhanis Has-Yun, Hilles, Ayah, Abbas, Phirdaous
Format:	Article
Language:	English
Published:	MDPI 2019
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/74977/ http://irep.iium.edu.my/74977/ http://irep.iium.edu.my/74977/ http://irep.iium.edu.my/74977/1/preprints201906.0003.v1.pdf

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recordtype	eprints
spelling	iium-749772019-11-08T07:06:42Z http://irep.iium.edu.my/74977/ Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Al-Qubaisi, Mothanna , Beh Yee Ismail, Norsharina Rasedee, Abdullah Yusof, Faridah Shahabuddin, Munira Al-khatib, Ma'an Kabbashi, Nassereldeen Ahmed Hashim, Yumi Zuhanis Has-Yun Hilles, Ayah Abbas, Phirdaous T Technology (General) Background and objectives: The initiation of apoptotic death in cancer cells is the aim of many chemotherapeutic strategies. As many anticancer agents have been discovered in natural plant sources, the search for novel phytochemicals displaying anticancer activities continues at a tremendous rate. This study was conducted to evaluate the effect of extractable polyphenols (EPP); non-extractable polyphenols (NEPP); nobiletin (NOB); tectorigenin (TEC) and persicogenin (PERS) from Barhi date palm kernels (BDPK) extracts on the induction of apoptosis in human lung cancer A549 and colon cancer HT29 cell lines. Materials and Methods: The anticancer activities were determined using MTT and trypan blue exclusion assays. Flow cytometry analysis was performed to study the cell viability, cell cycle arrest and apoptosis. The underlying mechanism of apoptosis induced by crude extracts and the purified compounds was investigated using caspase-3, -8 and -9 assays and the mitochondrial membrane potential assay. Results: The findings indicate that both BDPK extracts and the purified compounds do exert induced cell death on A549 and HT29 cells. The results observed from MTT- assay and trypan blue exclusion indicate that the cytotoxic effects of both plant extracts and the three isolated flavonoids are dose-dependent with higher cell death after 72 hours treatment. Treatment of human lung and colon cancer cells with EPP, NEPP, NOB, TEC and PERSI induced late stages of apoptosis as there was evidence of the DNA degradation and high percentage of the cells population was situated at sub-G1 phase, indicating a high population of apoptotic cells. Conclusions: Study of the apoptotic mechanism demonstrated that EPP and NEPP exhibited dependent mitochondrial signalling pathway as seen with caspase-9 and induced receptor-mediated (extrinsic) apoptotic pathway as seen with caspase-8. Therefore, our results suggest that BDPK extracts and the three isolated flavonoids could be a good candidate for developing anticancer agents. MDPI 2019-06 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/74977/1/preprints201906.0003.v1.pdf Mahmud, Israa Ali and Mirghani, Mohamed Elwathig Saeed and Al-Qubaisi, Mothanna and , Beh Yee and Ismail, Norsharina and Rasedee, Abdullah and Yusof, Faridah and Shahabuddin, Munira and Al-khatib, Ma'an and Kabbashi, Nassereldeen Ahmed and Hashim, Yumi Zuhanis Has-Yun and Hilles, Ayah and Abbas, Phirdaous (2019) Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds. Preprint. pp. 1-37. (Unpublished) https://www.preprints.org/manuscript/201906.0003/v1 10.20944/preprints201906.0003.v1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Al-Qubaisi, Mothanna , Beh Yee Ismail, Norsharina Rasedee, Abdullah Yusof, Faridah Shahabuddin, Munira Al-khatib, Ma'an Kabbashi, Nassereldeen Ahmed Hashim, Yumi Zuhanis Has-Yun Hilles, Ayah Abbas, Phirdaous Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds
description	Background and objectives: The initiation of apoptotic death in cancer cells is the aim of many chemotherapeutic strategies. As many anticancer agents have been discovered in natural plant sources, the search for novel phytochemicals displaying anticancer activities continues at a tremendous rate. This study was conducted to evaluate the effect of extractable polyphenols (EPP); non-extractable polyphenols (NEPP); nobiletin (NOB); tectorigenin (TEC) and persicogenin (PERS) from Barhi date palm kernels (BDPK) extracts on the induction of apoptosis in human lung cancer A549 and colon cancer HT29 cell lines. Materials and Methods: The anticancer activities were determined using MTT and trypan blue exclusion assays. Flow cytometry analysis was performed to study the cell viability, cell cycle arrest and apoptosis. The underlying mechanism of apoptosis induced by crude extracts and the purified compounds was investigated using caspase-3, -8 and -9 assays and the mitochondrial membrane potential assay. Results: The findings indicate that both BDPK extracts and the purified compounds do exert induced cell death on A549 and HT29 cells. The results observed from MTT- assay and trypan blue exclusion indicate that the cytotoxic effects of both plant extracts and the three isolated flavonoids are dose-dependent with higher cell death after 72 hours treatment. Treatment of human lung and colon cancer cells with EPP, NEPP, NOB, TEC and PERSI induced late stages of apoptosis as there was evidence of the DNA degradation and high percentage of the cells population was situated at sub-G1 phase, indicating a high population of apoptotic cells. Conclusions: Study of the apoptotic mechanism demonstrated that EPP and NEPP exhibited dependent mitochondrial signalling pathway as seen with caspase-9 and induced receptor-mediated (extrinsic) apoptotic pathway as seen with caspase-8. Therefore, our results suggest that BDPK extracts and the three isolated flavonoids could be a good candidate for developing anticancer agents.
format	Article
author	Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Al-Qubaisi, Mothanna , Beh Yee Ismail, Norsharina Rasedee, Abdullah Yusof, Faridah Shahabuddin, Munira Al-khatib, Ma'an Kabbashi, Nassereldeen Ahmed Hashim, Yumi Zuhanis Has-Yun Hilles, Ayah Abbas, Phirdaous
author_facet	Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Al-Qubaisi, Mothanna , Beh Yee Ismail, Norsharina Rasedee, Abdullah Yusof, Faridah Shahabuddin, Munira Al-khatib, Ma'an Kabbashi, Nassereldeen Ahmed Hashim, Yumi Zuhanis Has-Yun Hilles, Ayah Abbas, Phirdaous
author_sort	Mahmud, Israa Ali
title	Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds
title_short	Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds
title_full	Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds
title_fullStr	Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds
title_full_unstemmed	Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds
title_sort	induction of apoptosis and cell cycle arrest in two human cancer a549 and ht29 cells lines by barhi date palm kernels extracts and three isolated cytotoxic compounds
publisher	MDPI
publishDate	2019
url	http://irep.iium.edu.my/74977/ http://irep.iium.edu.my/74977/ http://irep.iium.edu.my/74977/ http://irep.iium.edu.my/74977/1/preprints201906.0003.v1.pdf
first_indexed	2023-09-18T21:46:07Z
last_indexed	2023-09-18T21:46:07Z
_version_	1777413461099151360

Induction of apoptosis and cell cycle arrest in two human cancer A549 and HT29 cells lines by Barhi date palm kernels extracts and three isolated cytotoxic compounds

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