Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method

Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method

The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chai...

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Bibliographic Details
Main Authors: Omar, Abdul Rahman, Mohd, Hair-Bejo, Ideris, Aini, Hamzah, Hairul Aini
Format: Patent
Language:English
Published: 2010
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/7475/
http://irep.iium.edu.my/7475/
http://irep.iium.edu.my/7475/
http://irep.iium.edu.my/7475/1/United_States_Patent__7659066.pdf
id iium-7475
recordtype eprints
spelling iium-74752011-12-21T01:13:45Z http://irep.iium.edu.my/7475/ Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method Omar, Abdul Rahman Mohd, Hair-Bejo Ideris, Aini Hamzah, Hairul Aini Q Science (General) The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values. 2010-02-09 Patent NonPeerReviewed application/pdf en http://irep.iium.edu.my/7475/1/United_States_Patent__7659066.pdf Omar, Abdul Rahman and Mohd, Hair-Bejo and Ideris, Aini and Hamzah, Hairul Aini (2010) Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method. 7,659,066. http://patft.uspto.gov/ 7,659,066
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic Q Science (General)
spellingShingle Q Science (General)
Omar, Abdul Rahman
Mohd, Hair-Bejo
Ideris, Aini
Hamzah, Hairul Aini
Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
description The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values.
format Patent
author Omar, Abdul Rahman
Mohd, Hair-Bejo
Ideris, Aini
Hamzah, Hairul Aini
author_facet Omar, Abdul Rahman
Mohd, Hair-Bejo
Ideris, Aini
Hamzah, Hairul Aini
author_sort Omar, Abdul Rahman
title Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
title_short Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
title_full Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
title_fullStr Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
title_full_unstemmed Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
title_sort detection and distinguishing infections bursal disease virus (ibdv) strains by molecular biology method
publishDate 2010
url http://irep.iium.edu.my/7475/
http://irep.iium.edu.my/7475/
http://irep.iium.edu.my/7475/
http://irep.iium.edu.my/7475/1/United_States_Patent__7659066.pdf
first_indexed 2023-09-18T20:16:53Z
last_indexed 2023-09-18T20:16:53Z
_version_ 1777407847523418112

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