Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excelle...

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Main Authors:	Samsudin, Nurhusna, Hashim, Yumi Zuhanis Has-Yun, Arifin, Mohd Azmir, Mel, Maizirwan, Hamzah, Mohd. Salleh, Nordin, Norshariza, Iis, Sopyan, Jimat, Dzun Noraini
Format:	Article
Language:	English English
Published:	Penerbit UTM Press 2017
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/1/2017%20-%20Statistical%20Optimization%20of%20Gelatin.pdf http://irep.iium.edu.my/74717/7/74717%20Statistical%20optimization%20of%20gelatin%20immobilisation%20SCOPUS.pdf

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spelling	iium-747172019-10-23T01:06:23Z http://irep.iium.edu.my/74717/ Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Hamzah, Mohd. Salleh Nordin, Norshariza Iis, Sopyan Jimat, Dzun Noraini QD Chemistry Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105 cells/ml) and UV/O3 treated PCL microcarrier (4.25×105 cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. ********************************************************************************************************************************************** Pengkulturan sel menggunakan “microcarriers” mungkin telah mengatasi kekangan sistem kultur sel konvensional. Walau bagaimanapun, cabaran utama untuk memastikan biokompatibiliti (keserasian permukaan) “microcarrier” dengan sel masih belum dapat diatasi sepenuhnya. Polycaprolactone (PCL), sejenis polimer terbiodegradasikan semakin mendapat perhatian berdasarkan ciri-ciri mekanikal yang sangat baik dan kinetik degradasi yang sesuai untuk pelbagai kegunaan. Namun begitu, moieti hidrokarbon tanpa kutub pada PCL menjadikan ia sub-optimum untuk pelekatan sel. Di dalam kajian ini, kumpulan berfungsi oksigen diperkenalkan melalui sistem penyinaran ultraungu dan pengudaraan ozon (system UV/O3) untuk membolehkan imobilisasi (pemegunan) gelatin secara kovalen pada permukaan “microcarrier” PCL dengan menggunakan 1-etil-3-(3- dimetilaminopropil) hidroklorida karbodiimid dan N-hidroksisuksinimid (EDAC/NHS). Keadaan optimum untuk memaksimumkan jumlah gelatin (1797.33 µg/g) pada permukaan PCL adalah seperti berikut: Nisbah1.5 COOH:EDAC, 10 mM NHS dan, 80 mg/ml gelatin. Penerbit UTM Press 2017-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/74717/1/2017%20-%20Statistical%20Optimization%20of%20Gelatin.pdf application/pdf en http://irep.iium.edu.my/74717/7/74717%20Statistical%20optimization%20of%20gelatin%20immobilisation%20SCOPUS.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Hamzah, Mohd. Salleh and Nordin, Norshariza and Iis, Sopyan and Jimat, Dzun Noraini (2017) Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility. Jurnal Teknologi, 79 (6). pp. 167-175. E-ISSN 2180-3722 https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/10417/6222 10.11113/jt.v79.10417
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Hamzah, Mohd. Salleh Nordin, Norshariza Iis, Sopyan Jimat, Dzun Noraini Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility
description	Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105 cells/ml) and UV/O3 treated PCL microcarrier (4.25×105 cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. ********************************************************************************************************************************************** Pengkulturan sel menggunakan “microcarriers” mungkin telah mengatasi kekangan sistem kultur sel konvensional. Walau bagaimanapun, cabaran utama untuk memastikan biokompatibiliti (keserasian permukaan) “microcarrier” dengan sel masih belum dapat diatasi sepenuhnya. Polycaprolactone (PCL), sejenis polimer terbiodegradasikan semakin mendapat perhatian berdasarkan ciri-ciri mekanikal yang sangat baik dan kinetik degradasi yang sesuai untuk pelbagai kegunaan. Namun begitu, moieti hidrokarbon tanpa kutub pada PCL menjadikan ia sub-optimum untuk pelekatan sel. Di dalam kajian ini, kumpulan berfungsi oksigen diperkenalkan melalui sistem penyinaran ultraungu dan pengudaraan ozon (system UV/O3) untuk membolehkan imobilisasi (pemegunan) gelatin secara kovalen pada permukaan “microcarrier” PCL dengan menggunakan 1-etil-3-(3- dimetilaminopropil) hidroklorida karbodiimid dan N-hidroksisuksinimid (EDAC/NHS). Keadaan optimum untuk memaksimumkan jumlah gelatin (1797.33 µg/g) pada permukaan PCL adalah seperti berikut: Nisbah1.5 COOH:EDAC, 10 mM NHS dan, 80 mg/ml gelatin.
format	Article
author	Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Hamzah, Mohd. Salleh Nordin, Norshariza Iis, Sopyan Jimat, Dzun Noraini
author_facet	Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Hamzah, Mohd. Salleh Nordin, Norshariza Iis, Sopyan Jimat, Dzun Noraini
author_sort	Samsudin, Nurhusna
title	Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility
title_short	Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility
title_full	Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility
title_fullStr	Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility
title_full_unstemmed	Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility
title_sort	statistical optimization of gelatin immobilisation on modified surface of pcl microcarrier to improve its compatibility
publisher	Penerbit UTM Press
publishDate	2017
url	http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/1/2017%20-%20Statistical%20Optimization%20of%20Gelatin.pdf http://irep.iium.edu.my/74717/7/74717%20Statistical%20optimization%20of%20gelatin%20immobilisation%20SCOPUS.pdf
first_indexed	2023-09-18T21:45:44Z
last_indexed	2023-09-18T21:45:44Z
_version_	1777413437379313664

Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility

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