Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility
Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excelle...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Penerbit UTM Press
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/ http://irep.iium.edu.my/74717/1/2017%20-%20Statistical%20Optimization%20of%20Gelatin.pdf http://irep.iium.edu.my/74717/7/74717%20Statistical%20optimization%20of%20gelatin%20immobilisation%20SCOPUS.pdf |
| Summary: | Growing cells on microcarriers may have overcome the limitation of conventional cell
culture system. However, the main challenge remains at ensuring the surface
biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has
received considerable attention because of its excellent mechanical properties and
degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety
renders it sub-optimal for cell attachment. In this present study, the aim was to improve
biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet
irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin
on the PCL microcarrier surface. Respond surface methodology was used as a statistical
approach to optimized parameters that effect the immobilization of gelatin. The
parameters used to maximized amount of gelatin immobilize were the mol ratio of
COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for
maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of
COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that
gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of
16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105
cells/ml) and UV/O3
treated PCL microcarrier (4.25×105
cells/ml). Therefore, immobilization of gelatin with
optimized parameters onto PCL microcarrier improved biocompatibility of PCL
microcarrier.
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Pengkulturan sel menggunakan “microcarriers” mungkin telah mengatasi kekangan sistem
kultur sel konvensional. Walau bagaimanapun, cabaran utama untuk memastikan
biokompatibiliti (keserasian permukaan) “microcarrier” dengan sel masih belum dapat
diatasi sepenuhnya. Polycaprolactone (PCL), sejenis polimer terbiodegradasikan semakin
mendapat perhatian berdasarkan ciri-ciri mekanikal yang sangat baik dan kinetik
degradasi yang sesuai untuk pelbagai kegunaan. Namun begitu, moieti hidrokarbon
tanpa kutub pada PCL menjadikan ia sub-optimum untuk pelekatan sel. Di dalam kajian
ini, kumpulan berfungsi oksigen diperkenalkan melalui sistem penyinaran ultraungu dan
pengudaraan ozon (system UV/O3) untuk membolehkan imobilisasi (pemegunan) gelatin
secara kovalen pada permukaan “microcarrier” PCL dengan menggunakan 1-etil-3-(3-
dimetilaminopropil) hidroklorida karbodiimid dan N-hidroksisuksinimid (EDAC/NHS).
Keadaan optimum untuk memaksimumkan jumlah gelatin (1797.33 µg/g) pada
permukaan PCL adalah seperti berikut: Nisbah1.5 COOH:EDAC, 10 mM NHS dan, 80 mg/ml
gelatin. |
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