Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells

INTRODUCTION: In wound healing mechanism, macrophages are involved as part of the cleaning of the microenvironment and thus promote the healing process. Several researches indicated that the depletion of macrophages could delay the re-epithelization and hinder the vascular formation due to the upreg...

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Main Authors:	Ab. Hadi, Hazrina, Kasmuri, Abdul Razak, Abdul Jalil, Mohd Azri
Format:	Conference or Workshop Item
Language:	English English English English English
Published:	2019
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/74696/ http://irep.iium.edu.my/74696/1/Cover%20page.pdf http://irep.iium.edu.my/74696/30/TUESDAY-11-JUNE-5.pdf http://irep.iium.edu.my/74696/29/immunomodulatory-effect-of-stingless-bee-1769.pdf http://irep.iium.edu.my/74696/3/Letter%20for%20OP.pdf http://irep.iium.edu.my/74696/5/Presentation.pdf

id	iium-74696
recordtype	eprints
spelling	iium-746962019-09-11T06:33:58Z http://irep.iium.edu.my/74696/ Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells Ab. Hadi, Hazrina Kasmuri, Abdul Razak Abdul Jalil, Mohd Azri RS192 Materia Medica-Pharmaceutical Technology INTRODUCTION: In wound healing mechanism, macrophages are involved as part of the cleaning of the microenvironment and thus promote the healing process. Several researches indicated that the depletion of macrophages could delay the re-epithelization and hinder the vascular formation due to the upregulation of inflammatory mediators and downregulation of growth factors. OBJECTIVES: The objectives of this study were to elucidate the effect of stingless bee honey on the wound healing mechanism by using LPS-activated RAW 264.7 macrophage model. MATERIALS AND METHODS: The inflammatory effect of murine macrophage cell line RAW 264.7 was stimulated by incubation with lipopolysaccharide (LPS). The macrophages were group into three different concentration of honey’s treatment, a blank and a control group. A competitive enzyme-linked immunosorbent assay (ELISA) kit was used to quantify the concentration of PGE2 while sandwich ELISA kit was used to measure the concentration of IL-1b, IL-6, and TNF-a. RESULTS: In acute inflammation, pro-inflammatory cytokines need to be upregulated to promote the healing processes. However, prolonged production of these pro-inflammatory cytokines can overdue the inflammatory process leads to chronic inflammation and hinder the healing process. Honey plays a unique role on these inflammation phase, which can act as a pro-inflammatory agent at one point and another point, worked as anti-inflammation agent to promote the wound healing, a term proposed by a few researchers as an immunomodulator. CONCLUSIONS: In conclusion, stingless bee honey has the immunomodulator properties to balance the production of pro-inflammatory cytokines when the level of cytokines is reduced or upsurge. 2019-06-11 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/74696/1/Cover%20page.pdf application/pdf en http://irep.iium.edu.my/74696/30/TUESDAY-11-JUNE-5.pdf application/pdf en http://irep.iium.edu.my/74696/29/immunomodulatory-effect-of-stingless-bee-1769.pdf application/pdf en http://irep.iium.edu.my/74696/3/Letter%20for%20OP.pdf application/pdf en http://irep.iium.edu.my/74696/5/Presentation.pdf Ab. Hadi, Hazrina and Kasmuri, Abdul Razak and Abdul Jalil, Mohd Azri (2019) Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells. In: 24th World Congress of Dermatology, 10th June - 15th June 2019, Milan, Italy. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English English English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Ab. Hadi, Hazrina Kasmuri, Abdul Razak Abdul Jalil, Mohd Azri Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells
description	INTRODUCTION: In wound healing mechanism, macrophages are involved as part of the cleaning of the microenvironment and thus promote the healing process. Several researches indicated that the depletion of macrophages could delay the re-epithelization and hinder the vascular formation due to the upregulation of inflammatory mediators and downregulation of growth factors. OBJECTIVES: The objectives of this study were to elucidate the effect of stingless bee honey on the wound healing mechanism by using LPS-activated RAW 264.7 macrophage model. MATERIALS AND METHODS: The inflammatory effect of murine macrophage cell line RAW 264.7 was stimulated by incubation with lipopolysaccharide (LPS). The macrophages were group into three different concentration of honey’s treatment, a blank and a control group. A competitive enzyme-linked immunosorbent assay (ELISA) kit was used to quantify the concentration of PGE2 while sandwich ELISA kit was used to measure the concentration of IL-1b, IL-6, and TNF-a. RESULTS: In acute inflammation, pro-inflammatory cytokines need to be upregulated to promote the healing processes. However, prolonged production of these pro-inflammatory cytokines can overdue the inflammatory process leads to chronic inflammation and hinder the healing process. Honey plays a unique role on these inflammation phase, which can act as a pro-inflammatory agent at one point and another point, worked as anti-inflammation agent to promote the wound healing, a term proposed by a few researchers as an immunomodulator. CONCLUSIONS: In conclusion, stingless bee honey has the immunomodulator properties to balance the production of pro-inflammatory cytokines when the level of cytokines is reduced or upsurge.
format	Conference or Workshop Item
author	Ab. Hadi, Hazrina Kasmuri, Abdul Razak Abdul Jalil, Mohd Azri
author_facet	Ab. Hadi, Hazrina Kasmuri, Abdul Razak Abdul Jalil, Mohd Azri
author_sort	Ab. Hadi, Hazrina
title	Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells
title_short	Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells
title_full	Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells
title_fullStr	Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells
title_full_unstemmed	Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells
title_sort	immunomodulatory effect of stingless bee honey on wound healing mechanism in lps-activated raw 264.7 cells
publishDate	2019
url	http://irep.iium.edu.my/74696/ http://irep.iium.edu.my/74696/1/Cover%20page.pdf http://irep.iium.edu.my/74696/30/TUESDAY-11-JUNE-5.pdf http://irep.iium.edu.my/74696/29/immunomodulatory-effect-of-stingless-bee-1769.pdf http://irep.iium.edu.my/74696/3/Letter%20for%20OP.pdf http://irep.iium.edu.my/74696/5/Presentation.pdf
first_indexed	2023-09-18T21:45:43Z
last_indexed	2023-09-18T21:45:43Z
_version_	1777413435804352512

Immunomodulatory effect of stingless bee honey on wound healing mechanism in LPS-activated RAW 264.7 cells

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