Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR

Previously, tomato leaves have been proved to be one of the potential anticancer agents. High Performance Liquid Chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation was used to predict the presents of group of metabolites and to assign the possibilit...

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Main Authors:	Wan Chik, Wan Dalila, Amid, Azura, Jamal, Parveen
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/7458/ http://irep.iium.edu.my/7458/ http://irep.iium.edu.my/7458/1/irep.iium.edu.my_7458_1_6.BME1_pg198-268_7458.pdf

id	iium-7458
recordtype	eprints
spelling	iium-74582012-01-19T06:00:27Z http://irep.iium.edu.my/7458/ Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen R Medicine (General) Previously, tomato leaves have been proved to be one of the potential anticancer agents. High Performance Liquid Chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation was used to predict the presents of group of metabolites and to assign the possibility of certain absorption bands, in which most of the peaks in partially purified tomato (Solanum lycopersicon) leaves methanol extract are attributable to the specific functional groups. The extraction was carried out in a shake flask by 82% methanol, 1:10(w/v)sample to solvent ratio, agitated at 22°C with 110 rpm within 24 hours. Later, the extract was partially purified by column chromatography. High Performance Liquid Chromatography (HPLC) is used to quantify the number of unknown component presents in the fraction. Then, a FT-IR Bruker Tensor 27 System was used during FTIR data acquisition. The collection of FTIR spectra was carried out at 16 scans with resolution of 4 cm-1using strong apodization in the frequency regions of 4,000–650 cm-1. The results support the premise that HPLC and FTIR spectroscopy are efficient and accurate methods for determining major and minor components presents in the extract. 2011-05-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/7458/1/irep.iium.edu.my_7458_1_6.BME1_pg198-268_7458.pdf Wan Chik, Wan Dalila and Amid, Azura and Jamal, Parveen (2011) Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR
description	Previously, tomato leaves have been proved to be one of the potential anticancer agents. High Performance Liquid Chromatography (HPLC) and Fourier transform infrared (FTIR) spectroscopic instrumentation was used to predict the presents of group of metabolites and to assign the possibility of certain absorption bands, in which most of the peaks in partially purified tomato (Solanum lycopersicon) leaves methanol extract are attributable to the specific functional groups. The extraction was carried out in a shake flask by 82% methanol, 1:10(w/v)sample to solvent ratio, agitated at 22°C with 110 rpm within 24 hours. Later, the extract was partially purified by column chromatography. High Performance Liquid Chromatography (HPLC) is used to quantify the number of unknown component presents in the fraction. Then, a FT-IR Bruker Tensor 27 System was used during FTIR data acquisition. The collection of FTIR spectra was carried out at 16 scans with resolution of 4 cm-1using strong apodization in the frequency regions of 4,000–650 cm-1. The results support the premise that HPLC and FTIR spectroscopy are efficient and accurate methods for determining major and minor components presents in the extract.
format	Conference or Workshop Item
author	Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen
author_facet	Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen
author_sort	Wan Chik, Wan Dalila
title	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR
title_short	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR
title_full	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR
title_fullStr	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR
title_full_unstemmed	Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR
title_sort	predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by hplc and ftir
publishDate	2011
url	http://irep.iium.edu.my/7458/ http://irep.iium.edu.my/7458/ http://irep.iium.edu.my/7458/1/irep.iium.edu.my_7458_1_6.BME1_pg198-268_7458.pdf
first_indexed	2023-09-18T20:16:52Z
last_indexed	2023-09-18T20:16:52Z
_version_	1777407845509103616

Predicting group of metabolites available in partially purified tomato leaves extract showing anticancer activity by HPLC and FTIR

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