Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia

A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia usi...

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Main Authors: Alaraidh, Ibrahim, Amid, Azura, Abd-El Aziem, Farouk, Ibrahim, Mohamed M., Bazaid, Salih, Greiner, Ralf, Alghunaim, Abdullah
Format: Article
Language:English
Published: Istanbul University Printing House 2011
Subjects:
Online Access:http://irep.iium.edu.my/7440/
http://irep.iium.edu.my/7440/
http://irep.iium.edu.my/7440/3/2116-6098-1-PB_7440.pdf
id iium-7440
recordtype eprints
spelling iium-74402012-04-25T01:30:39Z http://irep.iium.edu.my/7440/ Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia Alaraidh, Ibrahim Amid, Azura Abd-El Aziem, Farouk Ibrahim, Mohamed M. Bazaid, Salih Greiner, Ralf Alghunaim, Abdullah SB Plant culture A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia using PCR and agarose gel visualization. The samples were tested for the existence of the NOS Terminator. A total of 150 samples were tested out of which three samples tested positive as GM-rice which were retested in Malaysia. The presence of GMO rice in Saudi Arabia supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops. Istanbul University Printing House 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/7440/3/2116-6098-1-PB_7440.pdf Alaraidh, Ibrahim and Amid, Azura and Abd-El Aziem, Farouk and Ibrahim, Mohamed M. and Bazaid, Salih and Greiner, Ralf and Alghunaim, Abdullah (2011) Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia. IUFS Journal of Biology, 70 (1). pp. 11-17. ISSN 1300-7041 http://www.iudergi.com/tr/index.php/fenbiyoloji/article/viewFile/2116/1762
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic SB Plant culture
spellingShingle SB Plant culture
Alaraidh, Ibrahim
Amid, Azura
Abd-El Aziem, Farouk
Ibrahim, Mohamed M.
Bazaid, Salih
Greiner, Ralf
Alghunaim, Abdullah
Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
description A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia using PCR and agarose gel visualization. The samples were tested for the existence of the NOS Terminator. A total of 150 samples were tested out of which three samples tested positive as GM-rice which were retested in Malaysia. The presence of GMO rice in Saudi Arabia supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops.
format Article
author Alaraidh, Ibrahim
Amid, Azura
Abd-El Aziem, Farouk
Ibrahim, Mohamed M.
Bazaid, Salih
Greiner, Ralf
Alghunaim, Abdullah
author_facet Alaraidh, Ibrahim
Amid, Azura
Abd-El Aziem, Farouk
Ibrahim, Mohamed M.
Bazaid, Salih
Greiner, Ralf
Alghunaim, Abdullah
author_sort Alaraidh, Ibrahim
title Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
title_short Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
title_full Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
title_fullStr Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
title_full_unstemmed Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
title_sort detection of genetically modified rice: collaborative validation study of a pcr based detection of genetically modified rice oryza sativa commercially available in saudi arabia
publisher Istanbul University Printing House
publishDate 2011
url http://irep.iium.edu.my/7440/
http://irep.iium.edu.my/7440/
http://irep.iium.edu.my/7440/3/2116-6098-1-PB_7440.pdf
first_indexed 2023-09-18T20:16:50Z
last_indexed 2023-09-18T20:16:50Z
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