Antibacterial and antioxidant activities of extracts from Calophyllum ferrugineum and Calophyllum incrassatum = Aktiviti antibakteria dan antioksidan terhadap ekstrak daripada calophyllum ferrugineum dan calophyllum incrassatum
Calophyllum is a pan-tropical genus belongs to the Guttiferae family and locally known in Malaysia as ‘bintangor’. There has been a continual interest to further investigate the phytochemistry of Calophyllum sp because this genus is a rich source of active secondary metabolites which shows anti-HIV,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
The Malaysian Analytical Sciences Society
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/74310/ http://irep.iium.edu.my/74310/ http://irep.iium.edu.my/74310/ http://irep.iium.edu.my/74310/3/74310%20ANTIBACTERIAL%20AND%20ANTIOXIDANT%20ACTIVITIES%20OF%20EXTRACTS.pdf http://irep.iium.edu.my/74310/4/74310%20ANTIBACTERIAL%20AND%20ANTIOXIDANT%20ACTIVITIES%20OF%20EXTRACTS%20SCOPUS.pdf |
| Summary: | Calophyllum is a pan-tropical genus belongs to the Guttiferae family and locally known in Malaysia as ‘bintangor’. There has been a continual interest to further investigate the phytochemistry of Calophyllum sp because this genus is a rich source of active secondary metabolites which shows anti-HIV, cytotoxicity and antimicrobial properties. In this study, antibacterial and antioxidant activities of barks and leaves of C. ferrugineum and C. incrassatum were investigated. Cold extraction method employing dichloromethane, ethyl acetate and methanol as solvent was performed. All extracts were tested for their total phenolic content and antioxidant activities by DPPH radical scavenging and Ferric Reducing Antioxidant Power (FRAP) assays. The methanol extract from the leaves of C. ferrugineum showed the highest TPC value at 122.08 mg GAE/g and the lowest DPPH SC50 value at 11.80 μg/mL. The methanol extract from the barks of C. ferrugineum was found to have the highest FRAP value among all extracts. The antibacterial activity of all extracts was tested using minimum inhibition concentration (MIC) test against Bacillus subtilis, Staphylococcus aureus, Escheria coli and Pseudomonas aeruginosa. Only the dichloromethane extract from bark of C. ferrugineum showed moderate MIC value against Gram positive bacteria, B. subtilis and S. aureus at 125 μg/mL.
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Calophyllum adalah genus pan-tropika di dalam keluarga Guttiferae dan dikenali secara tempatan di Malaysia sebagai
‘bintangor’. Terdapat minat berterusan untuk mengkaji lebih mendalam tentang fitokimia Calophyllum sp kerana genus ini
adalah sumber yang kaya dengan metabolit sekunder aktif yang menunjukkan ciri-ciri anti-HIV, sitotoksiti dan antimikrob. Di
dalam kajian ini, aktiviti antibakteria dan antioksidan kulit batang dan daun C. ferrugineum dan C. incrassatum telah dikaji.
Kaedah pengekstrakan sejuk menggunakan diklorometana, etil asetat dan metanol sebagai pelarut telah dilakukan. Semua ekstrak
telah diuji untuk kandungan total fenolik dan aktiviti antioksidan melalui cerakin pemerangkapan radikal DPPH dan kuasa
antioksidan penurunan Ferik (FRAP). Ekstrak metanol daripada daun C. ferrugineum telah menunjukkan nilai TPC tertinggi
pada 122.08 mg GAE/g dan nilai SC50 DPPH terendah pada 11.80 µg/mL. Ekstrak metanol daripada kulit batang C. ferrugineum
telah menunjukkan nilai FRAP tertinggi antara semua ekstrak. Aktiviti antibakteria semua ekstrak telah diuji untuk kepekatan
rencatan minimum (MIC) terhadap Bacillus subtilis, Staphylococcus aureus, Escheria coli dan Pseudomonas aeruginosa. Hanya
ekstrak diklorometana kulit batang C. ferrugineum menunjukkan nilai MIC yang sederhana terhadap bakteria Gram positif, B.
subtilis dan S. aureus pada 125 µg/mL. |
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