Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture

Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of...

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Main Authors:	Chia, Ling Li, Jantan, Ibrahim, Chua, Kien Hui, Lam, Kok Wai, Rullah, Kamal, Mohd Aluwi, Mohd Fadhlizil Fasihi
Format:	Article
Language:	English
Published:	Frontiers 2016
Subjects:	RM Therapeutics. Pharmacology RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:	http://irep.iium.edu.my/74082/ http://irep.iium.edu.my/74082/ http://irep.iium.edu.my/74082/ http://irep.iium.edu.my/74082/1/Kamal%20Rullah%2012.pdf

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spelling	iium-740822019-09-18T08:20:30Z http://irep.iium.edu.my/74082/ Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture Chia, Ling Li Jantan, Ibrahim Chua, Kien Hui Lam, Kok Wai Rullah, Kamal Mohd Aluwi, Mohd Fadhlizil Fasihi RM Therapeutics. Pharmacology RS403 Materia Medica-Pharmaceutical Chemistry Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Pancreatic islets freshly isolated from male Wistar rats were treated with T3 for 1 h at 37°C in a microfluidic system with continuous operation. The cells were collected for total RNA extraction and reverse-transcribed, followed by measurement of insulin secretion-associated genes expression using quantitative real-time polymerase chain reaction. Molecular docking experiments were performed to gain insights on how the T3 bind to the receptors. Short-term exposure of δ- and γ-T3 to pancreatic β cells in a stimulant glucose condition (16.7 mM) significantly regulated preproinsulin mRNA levels and insulin gene transcription. In contrast, α-T3 possessed less ability in the activation of insulin synthesis level. Essentially, potassium chloride (KCl), a β cell membrane depolarising agent added into the treatment further enhanced the insulin production. δ- and γ-T3 revealed significantly higher quantitative expression in most of the insulin secretion-associated genes groups containing 16.7 mM glucose alone and 16.7 mM glucose with 30 mM KCl ranging from 600 to 1200 μM (p < 0.05). The findings suggest the potential of δ-T3 in regulating insulin synthesis and glucose-stimulated insulin secretion through triggering pathway especially in the presence of KCl. Frontiers 2016-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/74082/1/Kamal%20Rullah%2012.pdf Chia, Ling Li and Jantan, Ibrahim and Chua, Kien Hui and Lam, Kok Wai and Rullah, Kamal and Mohd Aluwi, Mohd Fadhlizil Fasihi (2016) Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Frontiers in Pharmacology, 7. pp. 1-17. ISSN 1663-9812 https://www.frontiersin.org/articles/10.3389/fphar.2016.00291/full 10.3389/fphar.2016.00291
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle	RM Therapeutics. Pharmacology RS403 Materia Medica-Pharmaceutical Chemistry Chia, Ling Li Jantan, Ibrahim Chua, Kien Hui Lam, Kok Wai Rullah, Kamal Mohd Aluwi, Mohd Fadhlizil Fasihi Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture
description	Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Pancreatic islets freshly isolated from male Wistar rats were treated with T3 for 1 h at 37°C in a microfluidic system with continuous operation. The cells were collected for total RNA extraction and reverse-transcribed, followed by measurement of insulin secretion-associated genes expression using quantitative real-time polymerase chain reaction. Molecular docking experiments were performed to gain insights on how the T3 bind to the receptors. Short-term exposure of δ- and γ-T3 to pancreatic β cells in a stimulant glucose condition (16.7 mM) significantly regulated preproinsulin mRNA levels and insulin gene transcription. In contrast, α-T3 possessed less ability in the activation of insulin synthesis level. Essentially, potassium chloride (KCl), a β cell membrane depolarising agent added into the treatment further enhanced the insulin production. δ- and γ-T3 revealed significantly higher quantitative expression in most of the insulin secretion-associated genes groups containing 16.7 mM glucose alone and 16.7 mM glucose with 30 mM KCl ranging from 600 to 1200 μM (p < 0.05). The findings suggest the potential of δ-T3 in regulating insulin synthesis and glucose-stimulated insulin secretion through triggering pathway especially in the presence of KCl.
format	Article
author	Chia, Ling Li Jantan, Ibrahim Chua, Kien Hui Lam, Kok Wai Rullah, Kamal Mohd Aluwi, Mohd Fadhlizil Fasihi
author_facet	Chia, Ling Li Jantan, Ibrahim Chua, Kien Hui Lam, Kok Wai Rullah, Kamal Mohd Aluwi, Mohd Fadhlizil Fasihi
author_sort	Chia, Ling Li
title	Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture
title_short	Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture
title_full	Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture
title_fullStr	Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture
title_full_unstemmed	Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture
title_sort	effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture
publisher	Frontiers
publishDate	2016
url	http://irep.iium.edu.my/74082/ http://irep.iium.edu.my/74082/ http://irep.iium.edu.my/74082/ http://irep.iium.edu.my/74082/1/Kamal%20Rullah%2012.pdf
first_indexed	2023-09-18T21:44:57Z
last_indexed	2023-09-18T21:44:57Z
_version_	1777413388097290240

Effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture

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