Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts

Introduction: Roselle (Hibiscus sabdariffa) is widely known for its benefits and medicinal properties. The aims of this study were to determine the total phenolic content (TPC) in H. sabdariffa extract, its antioxidant activity and relationship between TPC and its antioxidant scavenging capacity....

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Main Authors:	Haron, Normah, Mat Jusoh, Hanapi, Abul@Abdul Rahman, Syuhaida
Format:	Article
Language:	English
Published:	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia. 2019
Subjects:	Q Science (General) QD Chemistry
Online Access:	http://irep.iium.edu.my/73950/ http://irep.iium.edu.my/73950/ http://irep.iium.edu.my/73950/1/73950_Total%20phenolic%20content%20and%20antioxidant.pdf

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spelling	iium-739502019-08-15T06:29:56Z http://irep.iium.edu.my/73950/ Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts Haron, Normah Mat Jusoh, Hanapi Abul@Abdul Rahman, Syuhaida Q Science (General) QD Chemistry Introduction: Roselle (Hibiscus sabdariffa) is widely known for its benefits and medicinal properties. The aims of this study were to determine the total phenolic content (TPC) in H. sabdariffa extract, its antioxidant activity and relationship between TPC and its antioxidant scavenging capacity. Methods: The TPC value for each extract of free, bound soluble and bound insoluble phenolic acids was determined by using Folin-Ciocalteu assay. The antioxidant scavenging capacity was evaluated by using DPPH assay. Results: The results obtained in this study showed that bound soluble phenolic acids (7.63 + 0.28 mg GAE/g DW) had the highest TPC value than the free and insoluble phenolic acid extracts (2.78 + 0.20 mg GAE/g DW and 3.61 + 0.85 mg GAE/g DW, respectively) (p<0.05). As for the IC50 values, bound soluble phenolic acid extract exhibited the greatest value(0.62%) as compared to the free and bound insoluble phenolic acid extracts (0.90% and 1.14%, respectively). A positive correlation (R2 = 0.956) between three extracts (free, soluble and insoluble phenolic acids) of TPC and antioxidant radical scavenging capacity (DPPH) was obtained. Conclusion: It can be concluded that phenolic acids in H. sabdariffa extracts have an effect to the antioxidant scavenging capacity particularly in the bound soluble form. This finding proved that H. sabdariffa extracts could be a good source of natural antioxidant for food, cosmetic and pharmaceutical industries. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia. 2019-08-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/73950/1/73950_Total%20phenolic%20content%20and%20antioxidant.pdf Haron, Normah and Mat Jusoh, Hanapi and Abul@Abdul Rahman, Syuhaida (2019) Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts. International Journal of Allied Health Sciences, 3 (2). pp. 725-733. https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/183
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QD Chemistry
spellingShingle	Q Science (General) QD Chemistry Haron, Normah Mat Jusoh, Hanapi Abul@Abdul Rahman, Syuhaida Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts
description	Introduction: Roselle (Hibiscus sabdariffa) is widely known for its benefits and medicinal properties. The aims of this study were to determine the total phenolic content (TPC) in H. sabdariffa extract, its antioxidant activity and relationship between TPC and its antioxidant scavenging capacity. Methods: The TPC value for each extract of free, bound soluble and bound insoluble phenolic acids was determined by using Folin-Ciocalteu assay. The antioxidant scavenging capacity was evaluated by using DPPH assay. Results: The results obtained in this study showed that bound soluble phenolic acids (7.63 + 0.28 mg GAE/g DW) had the highest TPC value than the free and insoluble phenolic acid extracts (2.78 + 0.20 mg GAE/g DW and 3.61 + 0.85 mg GAE/g DW, respectively) (p<0.05). As for the IC50 values, bound soluble phenolic acid extract exhibited the greatest value(0.62%) as compared to the free and bound insoluble phenolic acid extracts (0.90% and 1.14%, respectively). A positive correlation (R2 = 0.956) between three extracts (free, soluble and insoluble phenolic acids) of TPC and antioxidant radical scavenging capacity (DPPH) was obtained. Conclusion: It can be concluded that phenolic acids in H. sabdariffa extracts have an effect to the antioxidant scavenging capacity particularly in the bound soluble form. This finding proved that H. sabdariffa extracts could be a good source of natural antioxidant for food, cosmetic and pharmaceutical industries.
format	Article
author	Haron, Normah Mat Jusoh, Hanapi Abul@Abdul Rahman, Syuhaida
author_facet	Haron, Normah Mat Jusoh, Hanapi Abul@Abdul Rahman, Syuhaida
author_sort	Haron, Normah
title	Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts
title_short	Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts
title_full	Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts
title_fullStr	Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts
title_full_unstemmed	Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts
title_sort	total phenolic content and antioxidant activity of roselle (hibiscus sabdariffa) extracts
publisher	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia.
publishDate	2019
url	http://irep.iium.edu.my/73950/ http://irep.iium.edu.my/73950/ http://irep.iium.edu.my/73950/1/73950_Total%20phenolic%20content%20and%20antioxidant.pdf
first_indexed	2023-09-18T21:44:50Z
last_indexed	2023-09-18T21:44:50Z
_version_	1777413380825415680

Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts

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