In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)

Introduction: Many efforts have been made to identify natural alternatives to reduce the side effects of cytotoxic drugs in cancer treatment. With this in mind, the current study aimed to investigate the cytotoxicity effects of one of the multifloral Malaysian honey, Kelulut honey (Trigona itama), a...

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Main Authors:	Mohd Salim, Siti Norfitrah, Ramakreshnana, Logaraj, Fong, Chng Saun, Abdul Wahab, Ridhwan, Abdull Rasad, Mohammad Syaiful Bahari
Format:	Article
Language:	English English
Published:	Elsevier 2019
Subjects:	R Medicine (General) RA0421 Public health. Hygiene. Preventive Medicine RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/73919/ http://irep.iium.edu.my/73919/ http://irep.iium.edu.my/73919/ http://irep.iium.edu.my/73919/13/73919%20In-vitro%20cytotoxicity%20of%20Trigona%20itama%20honey.pdf http://irep.iium.edu.my/73919/7/73919_In-vitro%20cytotoxicity%20of%20Trigona%20itama%20honey_scopus.pdf

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spelling	iium-739192019-11-25T15:48:05Z http://irep.iium.edu.my/73919/ In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549) Mohd Salim, Siti Norfitrah Ramakreshnana, Logaraj Fong, Chng Saun Abdul Wahab, Ridhwan Abdull Rasad, Mohammad Syaiful Bahari R Medicine (General) RA0421 Public health. Hygiene. Preventive Medicine RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics TX341 Nutrition. Foods and food supply Introduction: Many efforts have been made to identify natural alternatives to reduce the side effects of cytotoxic drugs in cancer treatment. With this in mind, the current study aimed to investigate the cytotoxicity effects of one of the multifloral Malaysian honey, Kelulut honey (Trigona itama), as a potential natural anticancer agent in stimulating apoptosis and cell cycle arrest to a human lung adenocarcinoma epithelial cell line (A549). Methods: The cells were treated with various concentrations of T. itama honey for 24, 48 and 72 hours. The cytotoxicity and cell viability were determined using trypan blue exclusion assay (TBEA) and flow cytometric analysis. Results: The moisture content in the analysed honey was 14.3 ± 0.8%, which was within the accepted international standard. The pH, electrical conductivity and proline content were 3.17 ± 0.02, 0.47 mS/cm - 0.55 mS/cm and 19.1 mg/kg - 20.2 mg/kg respectively. The findings demonstrated a significant dose and time-dependent inhibitory effect of T. itama honey with the maximum cytotoxic effects were observed at 72 hours with 20% concentration of T. itama honey, indicating 100% growth inhibition. Meanwhile, IC50 of T. itama honey treatment for A549 cells was determined as 0.62% v/v. Moreover, T. itama honey has a promising cytotoxic effect and proven capable of inducing cell cycle arrest at G2/M phase at 72 hours of exposure with IC50 concentration. Conclusion: This study provides a prefatory evidence on T. itama honey’s significant anticancer activity against human lung cancer cell lines. Elsevier 2019-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/73919/13/73919%20In-vitro%20cytotoxicity%20of%20Trigona%20itama%20honey.pdf application/pdf en http://irep.iium.edu.my/73919/7/73919_In-vitro%20cytotoxicity%20of%20Trigona%20itama%20honey_scopus.pdf Mohd Salim, Siti Norfitrah and Ramakreshnana, Logaraj and Fong, Chng Saun and Abdul Wahab, Ridhwan and Abdull Rasad, Mohammad Syaiful Bahari (2019) In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549). European Journal of Integrative Medicine, 30. pp. 1-8. ISSN 1876-3820 E-ISSN 1876-3839 https://www.sciencedirect.com/science/article/abs/pii/S187638201930383X 10.1016/j.eujim.2019.100955
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General) RA0421 Public health. Hygiene. Preventive Medicine RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics TX341 Nutrition. Foods and food supply
spellingShingle	R Medicine (General) RA0421 Public health. Hygiene. Preventive Medicine RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics TX341 Nutrition. Foods and food supply Mohd Salim, Siti Norfitrah Ramakreshnana, Logaraj Fong, Chng Saun Abdul Wahab, Ridhwan Abdull Rasad, Mohammad Syaiful Bahari In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)
description	Introduction: Many efforts have been made to identify natural alternatives to reduce the side effects of cytotoxic drugs in cancer treatment. With this in mind, the current study aimed to investigate the cytotoxicity effects of one of the multifloral Malaysian honey, Kelulut honey (Trigona itama), as a potential natural anticancer agent in stimulating apoptosis and cell cycle arrest to a human lung adenocarcinoma epithelial cell line (A549). Methods: The cells were treated with various concentrations of T. itama honey for 24, 48 and 72 hours. The cytotoxicity and cell viability were determined using trypan blue exclusion assay (TBEA) and flow cytometric analysis. Results: The moisture content in the analysed honey was 14.3 ± 0.8%, which was within the accepted international standard. The pH, electrical conductivity and proline content were 3.17 ± 0.02, 0.47 mS/cm - 0.55 mS/cm and 19.1 mg/kg - 20.2 mg/kg respectively. The findings demonstrated a significant dose and time-dependent inhibitory effect of T. itama honey with the maximum cytotoxic effects were observed at 72 hours with 20% concentration of T. itama honey, indicating 100% growth inhibition. Meanwhile, IC50 of T. itama honey treatment for A549 cells was determined as 0.62% v/v. Moreover, T. itama honey has a promising cytotoxic effect and proven capable of inducing cell cycle arrest at G2/M phase at 72 hours of exposure with IC50 concentration. Conclusion: This study provides a prefatory evidence on T. itama honey’s significant anticancer activity against human lung cancer cell lines.
format	Article
author	Mohd Salim, Siti Norfitrah Ramakreshnana, Logaraj Fong, Chng Saun Abdul Wahab, Ridhwan Abdull Rasad, Mohammad Syaiful Bahari
author_facet	Mohd Salim, Siti Norfitrah Ramakreshnana, Logaraj Fong, Chng Saun Abdul Wahab, Ridhwan Abdull Rasad, Mohammad Syaiful Bahari
author_sort	Mohd Salim, Siti Norfitrah
title	In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)
title_short	In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)
title_full	In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)
title_fullStr	In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)
title_full_unstemmed	In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)
title_sort	in-vitro cytotoxicity of trigona itama honey against human lung adenocarcinoma epithelial cell line (a549)
publisher	Elsevier
publishDate	2019
url	http://irep.iium.edu.my/73919/ http://irep.iium.edu.my/73919/ http://irep.iium.edu.my/73919/ http://irep.iium.edu.my/73919/13/73919%20In-vitro%20cytotoxicity%20of%20Trigona%20itama%20honey.pdf http://irep.iium.edu.my/73919/7/73919_In-vitro%20cytotoxicity%20of%20Trigona%20itama%20honey_scopus.pdf
first_indexed	2023-09-18T21:44:48Z
last_indexed	2023-09-18T21:44:48Z
_version_	1777413378320367616

In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)

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