Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults

Background: Pre-hypertension is associated with increased risk of cardiovascular disease. Chronic inflammation plays an important role in the pathophysiology of essential hypertension, with epigenetic dysregulation involvement. Nevertheless, the role of DNA methylation in prehypertensive state is...

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Main Authors:	Abdullah, Aszrin, Talib, Norlelawati A., Ab Rahman, Jamalludin, Wan Omar, Wan Fatein Nabeila, Mohd Shah, Azarisman Shah
Format:	Article
Language:	English English
Published:	Penerbit Universiti Sains Malaysia 2019
Subjects:	QP Physiology R Medicine (General) RB Pathology
Online Access:	http://irep.iium.edu.my/73865/ http://irep.iium.edu.my/73865/ http://irep.iium.edu.my/73865/ http://irep.iium.edu.my/73865/1/Leucocytic_DNA_Methylation_of_Interleukin-6_Promot%20MJMS.pdf http://irep.iium.edu.my/73865/7/73865_Leucocytic%20DNA%20Methylation%20of%20Interleukin_scopus.pdf

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spelling	iium-738652020-03-17T07:32:22Z http://irep.iium.edu.my/73865/ Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults Abdullah, Aszrin Talib, Norlelawati A. Ab Rahman, Jamalludin Wan Omar, Wan Fatein Nabeila Mohd Shah, Azarisman Shah QP Physiology R Medicine (General) RB Pathology Background: Pre-hypertension is associated with increased risk of cardiovascular disease. Chronic inflammation plays an important role in the pathophysiology of essential hypertension, with epigenetic dysregulation involvement. Nevertheless, the role of DNA methylation in prehypertensive state is unknown. The aim of this study was to investigate the association between DNA methylation level of interleukin-6 (IL-6) promoter in pre-hypertensive (PreHT) and normotensive (NT) young adults. Methods: A total of 80 NT and 80 PreHT healthy subjects aged between 18–45 years were recruited in Kuantan, Pahang, Malaysia using an observational cross-sectional study approach. DNA methylation level of IL-6 promoter in peripheral leukocytes were measured using bisulphite conversion and MethyLight assay. Results: There was no significant difference in age between NT and PreHT (P = 0.655). The mean blood pressure was 110(8)/73(5) mmHg in NT and 125(7)/82(5) mmHg in PreHT subjects. The IL-6 promoter methylation level was significantly lower in PreHT compared to NT subjects (P < 0.001). Conclusion: The current study demonstrates that hypomethylation of IL-6 promoter was associated with pre-hypertension in young adults. Thus, IL-6 methylation could be used as an early indicator for predicting hypertension and related risk of cardiovascular diseases in prehypertensive subjects. Gene expression and longitudinal studies are warranted to examine the methylation effect on IL-6 expression over time. Penerbit Universiti Sains Malaysia 2019-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/73865/1/Leucocytic_DNA_Methylation_of_Interleukin-6_Promot%20MJMS.pdf application/pdf en http://irep.iium.edu.my/73865/7/73865_Leucocytic%20DNA%20Methylation%20of%20Interleukin_scopus.pdf Abdullah, Aszrin and Talib, Norlelawati A. and Ab Rahman, Jamalludin and Wan Omar, Wan Fatein Nabeila and Mohd Shah, Azarisman Shah (2019) Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults. Malaysian Journal of Medical Sciences, 26 (6). pp. 46-54. ISSN 1394-195X E-ISSN 2180-4303 http://www.mjms.usm.my/MJMS26062019/05MJMS26062019_OA2.pdf 10.21315/mjms2019.26.6.5
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QP Physiology R Medicine (General) RB Pathology
spellingShingle	QP Physiology R Medicine (General) RB Pathology Abdullah, Aszrin Talib, Norlelawati A. Ab Rahman, Jamalludin Wan Omar, Wan Fatein Nabeila Mohd Shah, Azarisman Shah Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults
description	Background: Pre-hypertension is associated with increased risk of cardiovascular disease. Chronic inflammation plays an important role in the pathophysiology of essential hypertension, with epigenetic dysregulation involvement. Nevertheless, the role of DNA methylation in prehypertensive state is unknown. The aim of this study was to investigate the association between DNA methylation level of interleukin-6 (IL-6) promoter in pre-hypertensive (PreHT) and normotensive (NT) young adults. Methods: A total of 80 NT and 80 PreHT healthy subjects aged between 18–45 years were recruited in Kuantan, Pahang, Malaysia using an observational cross-sectional study approach. DNA methylation level of IL-6 promoter in peripheral leukocytes were measured using bisulphite conversion and MethyLight assay. Results: There was no significant difference in age between NT and PreHT (P = 0.655). The mean blood pressure was 110(8)/73(5) mmHg in NT and 125(7)/82(5) mmHg in PreHT subjects. The IL-6 promoter methylation level was significantly lower in PreHT compared to NT subjects (P < 0.001). Conclusion: The current study demonstrates that hypomethylation of IL-6 promoter was associated with pre-hypertension in young adults. Thus, IL-6 methylation could be used as an early indicator for predicting hypertension and related risk of cardiovascular diseases in prehypertensive subjects. Gene expression and longitudinal studies are warranted to examine the methylation effect on IL-6 expression over time.
format	Article
author	Abdullah, Aszrin Talib, Norlelawati A. Ab Rahman, Jamalludin Wan Omar, Wan Fatein Nabeila Mohd Shah, Azarisman Shah
author_facet	Abdullah, Aszrin Talib, Norlelawati A. Ab Rahman, Jamalludin Wan Omar, Wan Fatein Nabeila Mohd Shah, Azarisman Shah
author_sort	Abdullah, Aszrin
title	Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults
title_short	Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults
title_full	Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults
title_fullStr	Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults
title_full_unstemmed	Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults
title_sort	leucocytic dna methylation of interleukin-6 promoter reduction in pre-hypertensive young adults
publisher	Penerbit Universiti Sains Malaysia
publishDate	2019
url	http://irep.iium.edu.my/73865/ http://irep.iium.edu.my/73865/ http://irep.iium.edu.my/73865/ http://irep.iium.edu.my/73865/1/Leucocytic_DNA_Methylation_of_Interleukin-6_Promot%20MJMS.pdf http://irep.iium.edu.my/73865/7/73865_Leucocytic%20DNA%20Methylation%20of%20Interleukin_scopus.pdf
first_indexed	2023-09-18T21:44:44Z
last_indexed	2023-09-18T21:44:44Z
_version_	1777413373853433856

Leucocytic DNA methylation of Interleukin-6 promoter reduction in pre-hypertensive young adults

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