Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation

Citric acid (CA) has a high demand due to its various uses in the food and pharmaceutical industries. However, the natural supply of CAis minimal compared to its growing industrial demand. The increasingdemand for CA can be fulfilled by using biotechnological processes. Thi...

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Main Authors:	Almakki, Asma, Mirghani, Mohamed Elwathig Saeed, Kabbashi, Nassereldeen Ahmed
Format:	Article
Language:	English
Published:	2019
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/73831/ http://irep.iium.edu.my/73831/ http://irep.iium.edu.my/73831/1/PRODUCTION%20OF%20CITRIC%20ACID%20FROM%20SUGARCANE.pdf

id	iium-73831
recordtype	eprints
spelling	iium-738312019-11-13T10:04:40Z http://irep.iium.edu.my/73831/ Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation Almakki, Asma Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed TP155 Chemical engineering Citric acid (CA) has a high demand due to its various uses in the food and pharmaceutical industries. However, the natural supply of CAis minimal compared to its growing industrial demand. The increasingdemand for CA can be fulfilled by using biotechnological processes. This study utilized liquid state bioconversion by Aspergillus niger for CA production using sugarcane molasses as the primary substrate. Sugarcane molasses which is agricultural waste consists of significant proportion of organic matters such as lipids and carbohydrates. This makes sugarcane molasses as a potential and alternative source of producing CA at a lower cost. In this study, statistical optimization was applied to improve CA production using submerged fermentation in shake flasks. Aspergillus nigerwas cultured in potato dextrose agar. Then, inoculum spores were introduced into the fermentation media for a specific duration according to the experimental design from Central Composite Design (CCD) tool under Response Surface Methodology (RSM) in Design Expert 6.0 software. Three parameters were chosen to be optimized at 32⁰C i.e.agitation rate (160, 80, 200 rpm), substrate concentration (47, 60, 73%) and fermentation time (24, 72, 120 h). High Performance Liquid Chromatography (HPLC)and Fourier-transform infrared spectroscopy(FTIR) analyses were conducted to measure CA yield. The optimization study showed that the media incubated for 72 hours with a substrate concentration of 60% and an agitation speed of 180 rpm produced the highest CA yield(21.2 g/L).The analysis of variance (ANOVA) also showed that CCD quadraticmodel was significant with P-value< 0.0104 and R2is0.8964 2019-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/73831/1/PRODUCTION%20OF%20CITRIC%20ACID%20FROM%20SUGARCANE.pdf Almakki, Asma and Mirghani, Mohamed Elwathig Saeed and Kabbashi, Nassereldeen Ahmed (2019) Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation. Biological and Natural Resources Engineering Journal, 2 (1). pp. 47-55. E-ISSN 2637-0719 https://journals.iium.edu.my/bnrej/index.php/bnrej/article/view/31/14
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Almakki, Asma Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation
description	Citric acid (CA) has a high demand due to its various uses in the food and pharmaceutical industries. However, the natural supply of CAis minimal compared to its growing industrial demand. The increasingdemand for CA can be fulfilled by using biotechnological processes. This study utilized liquid state bioconversion by Aspergillus niger for CA production using sugarcane molasses as the primary substrate. Sugarcane molasses which is agricultural waste consists of significant proportion of organic matters such as lipids and carbohydrates. This makes sugarcane molasses as a potential and alternative source of producing CA at a lower cost. In this study, statistical optimization was applied to improve CA production using submerged fermentation in shake flasks. Aspergillus nigerwas cultured in potato dextrose agar. Then, inoculum spores were introduced into the fermentation media for a specific duration according to the experimental design from Central Composite Design (CCD) tool under Response Surface Methodology (RSM) in Design Expert 6.0 software. Three parameters were chosen to be optimized at 32⁰C i.e.agitation rate (160, 80, 200 rpm), substrate concentration (47, 60, 73%) and fermentation time (24, 72, 120 h). High Performance Liquid Chromatography (HPLC)and Fourier-transform infrared spectroscopy(FTIR) analyses were conducted to measure CA yield. The optimization study showed that the media incubated for 72 hours with a substrate concentration of 60% and an agitation speed of 180 rpm produced the highest CA yield(21.2 g/L).The analysis of variance (ANOVA) also showed that CCD quadraticmodel was significant with P-value< 0.0104 and R2is0.8964
format	Article
author	Almakki, Asma Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed
author_facet	Almakki, Asma Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed
author_sort	Almakki, Asma
title	Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation
title_short	Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation
title_full	Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation
title_fullStr	Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation
title_full_unstemmed	Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation
title_sort	production of citric acid from sugarcane molasses by aspergillus niger using submerged fermentation
publishDate	2019
url	http://irep.iium.edu.my/73831/ http://irep.iium.edu.my/73831/ http://irep.iium.edu.my/73831/1/PRODUCTION%20OF%20CITRIC%20ACID%20FROM%20SUGARCANE.pdf
first_indexed	2023-09-18T21:44:41Z
last_indexed	2023-09-18T21:44:41Z
_version_	1777413371282325504

Production of citric acid from sugarcane molasses by Aspergillus niger using submerged fermentation

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