Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs

Leukemia is classified as a malignant disease of hematopoietic stem cells (HSCs) that fails in cell differentiation but preserve their self-renewal. It is caused by genetic alterations and epigenetic modifications resulting in the activation or inactivation of particular genes for transcription. Epi...

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Main Authors:	Sudin, Aziee, Mohd Yassim, Haiyuni, Mohamed Yusof, Shafini, Shamsuddin, Shaharum, Abdul Wahab, Ridhwan, Johan, Muhammad Farid
Format:	Article
Language:	English English
Published:	Malaysian Society For Molecular Biology & Biotechnology 2019
Subjects:	RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/73822/ http://irep.iium.edu.my/73822/ http://irep.iium.edu.my/73822/7/73822%20Two-dimensional%20electrophoresis%20protein%20profiles.pdf http://irep.iium.edu.my/73822/8/73822%20Two-dimensional%20electrophoresis%20protein%20profiles%20SCOPUS.pdf

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spelling	iium-738222019-11-25T15:56:18Z http://irep.iium.edu.my/73822/ Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs Sudin, Aziee Mohd Yassim, Haiyuni Mohamed Yusof, Shafini Shamsuddin, Shaharum Abdul Wahab, Ridhwan Johan, Muhammad Farid RM300 Drugs and their action Leukemia is classified as a malignant disease of hematopoietic stem cells (HSCs) that fails in cell differentiation but preserve their self-renewal. It is caused by genetic alterations and epigenetic modifications resulting in the activation or inactivation of particular genes for transcription. Epigenetic causes changes in gene expression without any alteration in the DNA sequence. The most common epigenetic modifications are DNA methylation and histone acetylation. 5-Azacitidine (5-Aza) is a DNA methytransferase inhibitor (DNMTi) that inhibits DNA methyltransferase enzymes resulting in hypomethylation. Trichostatin A (TSA) is a histone deacetylase inhibitor which inhibits deacetylation of both histone and non-histone proteins resulting in chromatin relaxation. This present study focused on the alteration of proteome profile on 2D gel electrophoresis (2-DE) induced by 5-Aza and TSA in HL-60 and CCRF-CEM cell lines as in vitro model to represent acute promyelocytic leukemia (APL) and T-lymphoblastic leukemia (T-ALL), respectively. Total proteins of untreated and 5-Aza/TSA-treated HL-60 and CCRF-CEM cell lines were extracted using urea/thiourea buffer and stained with Coomassie Blue. Comparative analysis of untreated and 5-Aza/TSA-treated HL-60 and CCRF-CEM was performed by PDQuest software. Qualitative analysis identified 190-659 protein spots detected in untreated, 5-Aza and TSA-treated HL-60 and CCRF-CEM. Quantitative comparison analysis was analyzed by over 2-fold change in 5-Aza/TSA-treated cells compared to untreated. One and eight upregulated proteins were detected in 5-Aza and TSA-treated HL-60, respectively. While five and one upregulated proteins were detected in 5-Aza and TSA-treated CCRF-CEM, respectively. These preliminary results suggested that 5-Aza and TSA induced proteome profiles alterations due to their inhibition effects in HL-60 and CCRF-CEM cell lines. Malaysian Society For Molecular Biology & Biotechnology 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/73822/7/73822%20Two-dimensional%20electrophoresis%20protein%20profiles.pdf application/pdf en http://irep.iium.edu.my/73822/8/73822%20Two-dimensional%20electrophoresis%20protein%20profiles%20SCOPUS.pdf Sudin, Aziee and Mohd Yassim, Haiyuni and Mohamed Yusof, Shafini and Shamsuddin, Shaharum and Abdul Wahab, Ridhwan and Johan, Muhammad Farid (2019) Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs. Asia-Pacific Journal Molecular Biology and Biotechnology, 27 (3). pp. 10-23. ISSN 0128-7451 E-ISSN 2672-7277 http://www.msmbb.my/images/publication/volume_27/issue_3/02-Sudin-et-al.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM300 Drugs and their action
spellingShingle	RM300 Drugs and their action Sudin, Aziee Mohd Yassim, Haiyuni Mohamed Yusof, Shafini Shamsuddin, Shaharum Abdul Wahab, Ridhwan Johan, Muhammad Farid Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs
description	Leukemia is classified as a malignant disease of hematopoietic stem cells (HSCs) that fails in cell differentiation but preserve their self-renewal. It is caused by genetic alterations and epigenetic modifications resulting in the activation or inactivation of particular genes for transcription. Epigenetic causes changes in gene expression without any alteration in the DNA sequence. The most common epigenetic modifications are DNA methylation and histone acetylation. 5-Azacitidine (5-Aza) is a DNA methytransferase inhibitor (DNMTi) that inhibits DNA methyltransferase enzymes resulting in hypomethylation. Trichostatin A (TSA) is a histone deacetylase inhibitor which inhibits deacetylation of both histone and non-histone proteins resulting in chromatin relaxation. This present study focused on the alteration of proteome profile on 2D gel electrophoresis (2-DE) induced by 5-Aza and TSA in HL-60 and CCRF-CEM cell lines as in vitro model to represent acute promyelocytic leukemia (APL) and T-lymphoblastic leukemia (T-ALL), respectively. Total proteins of untreated and 5-Aza/TSA-treated HL-60 and CCRF-CEM cell lines were extracted using urea/thiourea buffer and stained with Coomassie Blue. Comparative analysis of untreated and 5-Aza/TSA-treated HL-60 and CCRF-CEM was performed by PDQuest software. Qualitative analysis identified 190-659 protein spots detected in untreated, 5-Aza and TSA-treated HL-60 and CCRF-CEM. Quantitative comparison analysis was analyzed by over 2-fold change in 5-Aza/TSA-treated cells compared to untreated. One and eight upregulated proteins were detected in 5-Aza and TSA-treated HL-60, respectively. While five and one upregulated proteins were detected in 5-Aza and TSA-treated CCRF-CEM, respectively. These preliminary results suggested that 5-Aza and TSA induced proteome profiles alterations due to their inhibition effects in HL-60 and CCRF-CEM cell lines.
format	Article
author	Sudin, Aziee Mohd Yassim, Haiyuni Mohamed Yusof, Shafini Shamsuddin, Shaharum Abdul Wahab, Ridhwan Johan, Muhammad Farid
author_facet	Sudin, Aziee Mohd Yassim, Haiyuni Mohamed Yusof, Shafini Shamsuddin, Shaharum Abdul Wahab, Ridhwan Johan, Muhammad Farid
author_sort	Sudin, Aziee
title	Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs
title_short	Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs
title_full	Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs
title_fullStr	Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs
title_full_unstemmed	Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs
title_sort	two-dimensional electrophoresis protein profiles of hl-60 and ccrf-cem cell lines treated with epigenetic modification drugs
publisher	Malaysian Society For Molecular Biology & Biotechnology
publishDate	2019
url	http://irep.iium.edu.my/73822/ http://irep.iium.edu.my/73822/ http://irep.iium.edu.my/73822/7/73822%20Two-dimensional%20electrophoresis%20protein%20profiles.pdf http://irep.iium.edu.my/73822/8/73822%20Two-dimensional%20electrophoresis%20protein%20profiles%20SCOPUS.pdf
first_indexed	2023-09-18T21:44:41Z
last_indexed	2023-09-18T21:44:41Z
_version_	1777413370830389248

Two-dimensional electrophoresis protein profiles of HL-60 and CCRF-CEM cell lines treated with epigenetic modification drugs

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