The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell

Stem cells provide various potential applications in regenerative medicine through its ability of self-renewal and differentiation. Among the various stem cells, dental pulp stem cells (DPSCs) have shown encouraging results in their ability to regenerate. Honey has been used in traditional culture...

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Main Authors:	Mohamad, Mohd Amin Marwan, Mazlan, Muhammad Alif, Ibrahim, Muhammad, Mat Yusof, Afzan, Ahmad Shamsuddin, Shamsul Azlin, Nik Hassan, Nik Fakhuruddin, Muhammad, Hussin, Md. Isa, Muhammad Lokman
Format:	Article
Language:	English English
Published:	Malaysian Society For Molecular Biology & Biotechnology 2019
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/72720/ http://irep.iium.edu.my/72720/ http://irep.iium.edu.my/72720/1/72720%20The%20effect%20of%20Malaysian%20stingless%20bee.pdf http://irep.iium.edu.my/72720/2/72720%20The%20effect%20of%20Malaysian%20stingless%20bee%20SCOPUS.pdf

id	iium-72720
recordtype	eprints
spelling	iium-727202020-01-29T19:16:19Z http://irep.iium.edu.my/72720/ The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell Mohamad, Mohd Amin Marwan Mazlan, Muhammad Alif Ibrahim, Muhammad Mat Yusof, Afzan Ahmad Shamsuddin, Shamsul Azlin Nik Hassan, Nik Fakhuruddin Muhammad, Hussin Md. Isa, Muhammad Lokman TP248.13 Biotechnology Stem cells provide various potential applications in regenerative medicine through its ability of self-renewal and differentiation. Among the various stem cells, dental pulp stem cells (DPSCs) have shown encouraging results in their ability to regenerate. Honey has been used in traditional culture as a natural medicine in supporting wound healing. Yet, very few studies on honey were conducted for its potential as a proliferative agent for stem cells. The aim of this study is to evaluate the stability of two Trigona spp. honeys (1 and 2) added in culture media and its proliferative effect on DPSCs. Both honeys were diluted with standard culture medium through dilution process to prepare the concentrations of 0.01%, 0.04%, 0.10% and 0.25%. DPSCs were treated with the diluted honeys for 24 hours. The proliferative activity was determined through the images taken using an inverted microscope for every six hours. In addition, the MTT assay was conducted to determine the cell viability of DPSCs when treated with both honey 1 and 2 at various concentrations. The results showed a stable culture media added with honey for three days and a dose-dependent proliferative effect of both Trigona spp. honey samples on DPSCs. Optimum proliferative effects were observed at 24 hours for both Trigona spp. honey 1 and 2 on DPSCs. The optimum concentration of Trigona spp. honey 1 was from 0.04% to 0.10% and Trigona spp. honey 2 was below 0.01%. It is concluded that Trigona spp. honey has a promising proliferative effect on DPSCs. Malaysian Society For Molecular Biology & Biotechnology 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72720/1/72720%20The%20effect%20of%20Malaysian%20stingless%20bee.pdf application/pdf en http://irep.iium.edu.my/72720/2/72720%20The%20effect%20of%20Malaysian%20stingless%20bee%20SCOPUS.pdf Mohamad, Mohd Amin Marwan and Mazlan, Muhammad Alif and Ibrahim, Muhammad and Mat Yusof, Afzan and Ahmad Shamsuddin, Shamsul Azlin and Nik Hassan, Nik Fakhuruddin and Muhammad, Hussin and Md. Isa, Muhammad Lokman (2019) The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell. Asia Pacific Journal of Molecular Biology and Technology, 27 (1). pp. 10-19. ISSN 0128-7451 E-ISSN 2672-7277 http://www.msmbb.my/images/publication/volume_27/issue_1/02-Mohamad-et-al.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohamad, Mohd Amin Marwan Mazlan, Muhammad Alif Ibrahim, Muhammad Mat Yusof, Afzan Ahmad Shamsuddin, Shamsul Azlin Nik Hassan, Nik Fakhuruddin Muhammad, Hussin Md. Isa, Muhammad Lokman The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell
description	Stem cells provide various potential applications in regenerative medicine through its ability of self-renewal and differentiation. Among the various stem cells, dental pulp stem cells (DPSCs) have shown encouraging results in their ability to regenerate. Honey has been used in traditional culture as a natural medicine in supporting wound healing. Yet, very few studies on honey were conducted for its potential as a proliferative agent for stem cells. The aim of this study is to evaluate the stability of two Trigona spp. honeys (1 and 2) added in culture media and its proliferative effect on DPSCs. Both honeys were diluted with standard culture medium through dilution process to prepare the concentrations of 0.01%, 0.04%, 0.10% and 0.25%. DPSCs were treated with the diluted honeys for 24 hours. The proliferative activity was determined through the images taken using an inverted microscope for every six hours. In addition, the MTT assay was conducted to determine the cell viability of DPSCs when treated with both honey 1 and 2 at various concentrations. The results showed a stable culture media added with honey for three days and a dose-dependent proliferative effect of both Trigona spp. honey samples on DPSCs. Optimum proliferative effects were observed at 24 hours for both Trigona spp. honey 1 and 2 on DPSCs. The optimum concentration of Trigona spp. honey 1 was from 0.04% to 0.10% and Trigona spp. honey 2 was below 0.01%. It is concluded that Trigona spp. honey has a promising proliferative effect on DPSCs.
format	Article
author	Mohamad, Mohd Amin Marwan Mazlan, Muhammad Alif Ibrahim, Muhammad Mat Yusof, Afzan Ahmad Shamsuddin, Shamsul Azlin Nik Hassan, Nik Fakhuruddin Muhammad, Hussin Md. Isa, Muhammad Lokman
author_facet	Mohamad, Mohd Amin Marwan Mazlan, Muhammad Alif Ibrahim, Muhammad Mat Yusof, Afzan Ahmad Shamsuddin, Shamsul Azlin Nik Hassan, Nik Fakhuruddin Muhammad, Hussin Md. Isa, Muhammad Lokman
author_sort	Mohamad, Mohd Amin Marwan
title	The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell
title_short	The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell
title_full	The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell
title_fullStr	The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell
title_full_unstemmed	The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell
title_sort	effect of malaysian stingless bee, trigona spp. honey in promoting proliferation of the undifferentiated stem cell
publisher	Malaysian Society For Molecular Biology & Biotechnology
publishDate	2019
url	http://irep.iium.edu.my/72720/ http://irep.iium.edu.my/72720/ http://irep.iium.edu.my/72720/1/72720%20The%20effect%20of%20Malaysian%20stingless%20bee.pdf http://irep.iium.edu.my/72720/2/72720%20The%20effect%20of%20Malaysian%20stingless%20bee%20SCOPUS.pdf
first_indexed	2023-09-18T21:43:04Z
last_indexed	2023-09-18T21:43:04Z
_version_	1777413269161508864

The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell

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