Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose

Pre-treatment of lignocellulosic biomass is essential for the cost-effective saccharification process to produce fermentable sugars. In this study, Deep Eutectic Solvents (DESs) and halophilic cellulase system were used as a new green and cost-effective approach for lignocellulose hydrolysis. The...

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Main Authors:	Nagoor Gunny, Ahmad Anas, Arbain, Dachyar, Javed, Muhammad, Baghaei-Yazdi, Namdar, Gopinath, Subash C.B., Jamal, Parveen
Format:	Article
Language:	English English English
Published:	Elsevier 2019
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/1/72716%20Deep%20eutectic%20solvents-halophilic%20cellulase%20system.pdf http://irep.iium.edu.my/72716/2/72716%20Deep%20eutectic%20solvents-halophilic%20cellulase%20system%20SCOPUS.pdf http://irep.iium.edu.my/72716/13/72716_Deep%20eutectic%20solvents-halophilic%20cellulase%20system%20An%20efficient%20route%20for%20in%20situ%20saccharification%20of%20lignocellulose_wos.pdf

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spelling	iium-727162019-09-29T14:55:34Z http://irep.iium.edu.my/72716/ Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose Nagoor Gunny, Ahmad Anas Arbain, Dachyar Javed, Muhammad Baghaei-Yazdi, Namdar Gopinath, Subash C.B. Jamal, Parveen TP248.13 Biotechnology Pre-treatment of lignocellulosic biomass is essential for the cost-effective saccharification process to produce fermentable sugars. In this study, Deep Eutectic Solvents (DESs) and halophilic cellulase system were used as a new green and cost-effective approach for lignocellulose hydrolysis. The stability and compatibility between DES and halophilic cellulase for lignocellulose hydrolysis were investigated by monitoring the stability of halophilic cellulase in the occurrence of different concentrations of DESs. It was found that halophilic cellulase showed higher stability in the occurrence of 10–20% (v/v) DES. It has been noticed that 20% of DESs, enhanced 2–3 folds in the release of glucose. The compatibility of the DES-halophilic cellulase system has been further evaluated and improved the saccharification efficiency even at high solid loadings. Using the system , saccharification of the rice husk produced three folds of glucose higher than the untreated sample. The yield was estimated to be higher than 1 mM of glucose using the halophilic cellulase -DES system with the hydrolysis for 36 h. DESs-halophilic cellulase system offers a good alternative compared to the available lignocelluloses pre-treatment method in terms of cost, environmental and Elsevier 2019-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72716/1/72716%20Deep%20eutectic%20solvents-halophilic%20cellulase%20system.pdf application/pdf en http://irep.iium.edu.my/72716/2/72716%20Deep%20eutectic%20solvents-halophilic%20cellulase%20system%20SCOPUS.pdf application/pdf en http://irep.iium.edu.my/72716/13/72716_Deep%20eutectic%20solvents-halophilic%20cellulase%20system%20An%20efficient%20route%20for%20in%20situ%20saccharification%20of%20lignocellulose_wos.pdf Nagoor Gunny, Ahmad Anas and Arbain, Dachyar and Javed, Muhammad and Baghaei-Yazdi, Namdar and Gopinath, Subash C.B. and Jamal, Parveen (2019) Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose. Process Biochemistry, 81. pp. 99-103. ISSN 1359-5113 https://www.sciencedirect.com/science/article/pii/S1359511319301710 10.1016/j.procbio.2019.03.003
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Nagoor Gunny, Ahmad Anas Arbain, Dachyar Javed, Muhammad Baghaei-Yazdi, Namdar Gopinath, Subash C.B. Jamal, Parveen Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose
description	Pre-treatment of lignocellulosic biomass is essential for the cost-effective saccharification process to produce fermentable sugars. In this study, Deep Eutectic Solvents (DESs) and halophilic cellulase system were used as a new green and cost-effective approach for lignocellulose hydrolysis. The stability and compatibility between DES and halophilic cellulase for lignocellulose hydrolysis were investigated by monitoring the stability of halophilic cellulase in the occurrence of different concentrations of DESs. It was found that halophilic cellulase showed higher stability in the occurrence of 10–20% (v/v) DES. It has been noticed that 20% of DESs, enhanced 2–3 folds in the release of glucose. The compatibility of the DES-halophilic cellulase system has been further evaluated and improved the saccharification efficiency even at high solid loadings. Using the system , saccharification of the rice husk produced three folds of glucose higher than the untreated sample. The yield was estimated to be higher than 1 mM of glucose using the halophilic cellulase -DES system with the hydrolysis for 36 h. DESs-halophilic cellulase system offers a good alternative compared to the available lignocelluloses pre-treatment method in terms of cost, environmental and
format	Article
author	Nagoor Gunny, Ahmad Anas Arbain, Dachyar Javed, Muhammad Baghaei-Yazdi, Namdar Gopinath, Subash C.B. Jamal, Parveen
author_facet	Nagoor Gunny, Ahmad Anas Arbain, Dachyar Javed, Muhammad Baghaei-Yazdi, Namdar Gopinath, Subash C.B. Jamal, Parveen
author_sort	Nagoor Gunny, Ahmad Anas
title	Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose
title_short	Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose
title_full	Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose
title_fullStr	Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose
title_full_unstemmed	Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose
title_sort	deep eutectic solvents-halophilic cellulase system: an efficient route for in situ saccharification of lignocellulose
publisher	Elsevier
publishDate	2019
url	http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/1/72716%20Deep%20eutectic%20solvents-halophilic%20cellulase%20system.pdf http://irep.iium.edu.my/72716/2/72716%20Deep%20eutectic%20solvents-halophilic%20cellulase%20system%20SCOPUS.pdf http://irep.iium.edu.my/72716/13/72716_Deep%20eutectic%20solvents-halophilic%20cellulase%20system%20An%20efficient%20route%20for%20in%20situ%20saccharification%20of%20lignocellulose_wos.pdf
first_indexed	2023-09-18T21:43:03Z
last_indexed	2023-09-18T21:43:03Z
_version_	1777413268571160576

Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose

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