Deep eutectic solvents-halophilic cellulase system: An efficient route for in situ saccharification of lignocellulose
Pre-treatment of lignocellulosic biomass is essential for the cost-effective saccharification process to produce fermentable sugars. In this study, Deep Eutectic Solvents (DESs) and halophilic cellulase system were used as a new green and cost-effective approach for lignocellulose hydrolysis. The...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Elsevier
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/ http://irep.iium.edu.my/72716/1/72716%20Deep%20eutectic%20solvents-halophilic%20cellulase%20system.pdf http://irep.iium.edu.my/72716/2/72716%20Deep%20eutectic%20solvents-halophilic%20cellulase%20system%20SCOPUS.pdf http://irep.iium.edu.my/72716/13/72716_Deep%20eutectic%20solvents-halophilic%20cellulase%20system%20An%20efficient%20route%20for%20in%20situ%20saccharification%20of%20lignocellulose_wos.pdf |
| Summary: | Pre-treatment of lignocellulosic biomass is essential for the cost-effective saccharification process to produce
fermentable sugars. In this study, Deep Eutectic Solvents (DESs) and halophilic cellulase system were used as a
new green and cost-effective approach for lignocellulose hydrolysis. The stability and compatibility between DES and
halophilic cellulase for lignocellulose hydrolysis were investigated by monitoring the stability of halophilic cellulase
in the occurrence of different concentrations of DESs. It was found that halophilic cellulase showed higher stability
in the occurrence of 10–20% (v/v) DES. It has been noticed that 20% of DESs, enhanced 2–3 folds in the release of
glucose. The compatibility of the DES-halophilic cellulase system has been further evaluated and improved the
saccharification efficiency even at high solid loadings. Using the system , saccharification of the rice husk produced
three folds of glucose higher than the untreated sample. The yield was estimated to be higher than 1 mM of glucose
using the halophilic cellulase -DES system with the hydrolysis for 36 h. DESs-halophilic cellulase system offers a
good alternative compared to the available lignocelluloses pre-treatment method in terms of cost, environmental and |
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