Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities

Purpose – The purpose of this paper is to extract, characterise and quantify curcumin from selected Zingiberaceae of “kunyit” or turmeric (Curcuma longa), “temu lawak” or Javanese turmeric (Curcuma xanthorrhiza), “temu pauh” (Curcuma mangga), “lempoyang” (Zingiber zerumbet) and “bonglai” (Zingiber...

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Main Authors:	Othman, Rashidi, Abdurasid, Mohd Akram, Mahmad, Noraini, Ahmad Fadzlillah, Nurrulhidayah
Format:	Article
Language:	English English
Published:	Emerald Group Publishing Ltd. 2019
Subjects:	S Agriculture (General) SB Plant culture TP Chemical technology TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc
Online Access:	http://irep.iium.edu.my/72714/ http://irep.iium.edu.my/72714/ http://irep.iium.edu.my/72714/ http://irep.iium.edu.my/72714/1/72714_Alkaline-based%20curcumin%20extraction.pdf http://irep.iium.edu.my/72714/2/72714_Alkaline-based%20curcumin%20extraction_SCOPUS.pdf

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recordtype	eprints
spelling	iium-727142019-08-09T08:57:55Z http://irep.iium.edu.my/72714/ Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities Othman, Rashidi Abdurasid, Mohd Akram Mahmad, Noraini Ahmad Fadzlillah, Nurrulhidayah S Agriculture (General) SB Plant culture TP Chemical technology TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Purpose – The purpose of this paper is to extract, characterise and quantify curcumin from selected Zingiberaceae of “kunyit” or turmeric (Curcuma longa), “temu lawak” or Javanese turmeric (Curcuma xanthorrhiza), “temu pauh” (Curcuma mangga), “lempoyang” (Zingiber zerumbet) and “bonglai” (Zingiber cassumunar) using alkaline and chemical-based extraction method for antimicrobial and antioxidant activities. Design/methodology/approach – Through the alkaline-based extraction method, all parts of rhizome samples were freeze-dried for 72 h before grounded into a fine powder and kept at �20°C. The powdered sample (0.1 g) was weighed and placed in a 50mL tube. About 20 mL of 2M NaOH solution was added into the tube. The solution was allowed to stand for 30 min. Then, 20 mL of ethyl acetate was added into the tube. The solution was mixed well then centrifuged at 13,500 rpm for 3 min. The upper layer was collected using a pipette. The process was repeated until the upper layer became almost colourless. The collected ethyl acetate solution was concentrated using a rotary evaporator to remove the ethyl acetate from the extracted compound. The concentrated curcumin was placed in a universal bottle, which was then dried from the remaining ethyl acetate using nitrogen drying process. The dried curcumin was then stored inside the freezer at �208C. The antimicrobial activities were using agar diffusion method against bacterial and fungi, while the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Findings – All the samples successfully showed a single peak (curcumin) that gained from the high-performance liquid chromatography (HPLC) chromatogram analysis (at 425 nm) using the alkaline-based extraction method and the highest curcumin content was in turmeric (12.9561.07mg/g DW). At 10.0 mg/mL curcumin concentration, the best antibacterial activity was against on methicillin-resistant Staphylococcus aureus (MRSA) with 7.5060.71mm inhibition zone, while the best antifungal activity was against on Aspergillus niger with 8.0060.41mm inhibition zone. The DPPH antioxidant test resulted in the highest inhibition (110.41 percent) was at 0.25mg/mL curcumin concentration. Originality/value – Through HPLC analysis, all samples successfully showed a single peak of curcumin at 425 nm. The total carotenoid determination from turmeric revealed that the content of the sample was substantially higher using alkaline-based extraction (18.4060.07 mg/g DW) compared to chemical-based extraction (9.4260.20 mg/g 6 SD). Emerald Group Publishing Ltd. 2019-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72714/1/72714_Alkaline-based%20curcumin%20extraction.pdf application/pdf en http://irep.iium.edu.my/72714/2/72714_Alkaline-based%20curcumin%20extraction_SCOPUS.pdf Othman, Rashidi and Abdurasid, Mohd Akram and Mahmad, Noraini and Ahmad Fadzlillah, Nurrulhidayah (2019) Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities. Pigment & Resin Technology, 48 (4). pp. 289-296. ISSN 0369-9420 https://www.emerald.com/insight/content/doi/10.1108/PRT-08-2018-0071/full/html 10.1108/PRT-08-2018-0071
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	S Agriculture (General) SB Plant culture TP Chemical technology TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc
spellingShingle	S Agriculture (General) SB Plant culture TP Chemical technology TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Othman, Rashidi Abdurasid, Mohd Akram Mahmad, Noraini Ahmad Fadzlillah, Nurrulhidayah Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities
description	Purpose – The purpose of this paper is to extract, characterise and quantify curcumin from selected Zingiberaceae of “kunyit” or turmeric (Curcuma longa), “temu lawak” or Javanese turmeric (Curcuma xanthorrhiza), “temu pauh” (Curcuma mangga), “lempoyang” (Zingiber zerumbet) and “bonglai” (Zingiber cassumunar) using alkaline and chemical-based extraction method for antimicrobial and antioxidant activities. Design/methodology/approach – Through the alkaline-based extraction method, all parts of rhizome samples were freeze-dried for 72 h before grounded into a fine powder and kept at �20°C. The powdered sample (0.1 g) was weighed and placed in a 50mL tube. About 20 mL of 2M NaOH solution was added into the tube. The solution was allowed to stand for 30 min. Then, 20 mL of ethyl acetate was added into the tube. The solution was mixed well then centrifuged at 13,500 rpm for 3 min. The upper layer was collected using a pipette. The process was repeated until the upper layer became almost colourless. The collected ethyl acetate solution was concentrated using a rotary evaporator to remove the ethyl acetate from the extracted compound. The concentrated curcumin was placed in a universal bottle, which was then dried from the remaining ethyl acetate using nitrogen drying process. The dried curcumin was then stored inside the freezer at �208C. The antimicrobial activities were using agar diffusion method against bacterial and fungi, while the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Findings – All the samples successfully showed a single peak (curcumin) that gained from the high-performance liquid chromatography (HPLC) chromatogram analysis (at 425 nm) using the alkaline-based extraction method and the highest curcumin content was in turmeric (12.9561.07mg/g DW). At 10.0 mg/mL curcumin concentration, the best antibacterial activity was against on methicillin-resistant Staphylococcus aureus (MRSA) with 7.5060.71mm inhibition zone, while the best antifungal activity was against on Aspergillus niger with 8.0060.41mm inhibition zone. The DPPH antioxidant test resulted in the highest inhibition (110.41 percent) was at 0.25mg/mL curcumin concentration. Originality/value – Through HPLC analysis, all samples successfully showed a single peak of curcumin at 425 nm. The total carotenoid determination from turmeric revealed that the content of the sample was substantially higher using alkaline-based extraction (18.4060.07 mg/g DW) compared to chemical-based extraction (9.4260.20 mg/g 6 SD).
format	Article
author	Othman, Rashidi Abdurasid, Mohd Akram Mahmad, Noraini Ahmad Fadzlillah, Nurrulhidayah
author_facet	Othman, Rashidi Abdurasid, Mohd Akram Mahmad, Noraini Ahmad Fadzlillah, Nurrulhidayah
author_sort	Othman, Rashidi
title	Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities
title_short	Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities
title_full	Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities
title_fullStr	Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities
title_full_unstemmed	Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities
title_sort	alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities
publisher	Emerald Group Publishing Ltd.
publishDate	2019
url	http://irep.iium.edu.my/72714/ http://irep.iium.edu.my/72714/ http://irep.iium.edu.my/72714/ http://irep.iium.edu.my/72714/1/72714_Alkaline-based%20curcumin%20extraction.pdf http://irep.iium.edu.my/72714/2/72714_Alkaline-based%20curcumin%20extraction_SCOPUS.pdf
first_indexed	2023-09-18T21:43:03Z
last_indexed	2023-09-18T21:43:03Z
_version_	1777413268279656448

Alkaline-based curcumin extraction from selected zingiberaceae for antimicrobial and antioxidant activities

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