Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system

Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities...

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Main Authors:	Zakaria, Zainul Amiruddin, Mahmood, Nor Diyana, Omar, Maizatul Hasyima, Bakhtiar, M. Taher, Basir, Rusliza
Format:	Article
Language:	English English
Published:	Taylor and Francis 2019
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/1/Pharmbiol_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4%20induced%20liver%20injury%20possible%20synergistic%20action%20of%20flavonoids%20and%20volatile%20bioactive.pdf http://irep.iium.edu.my/72035/7/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4_scopus.pdf

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spelling	iium-720352019-07-12T01:53:30Z http://irep.iium.edu.my/72035/ Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system Zakaria, Zainul Amiruddin Mahmood, Nor Diyana Omar, Maizatul Hasyima Bakhtiar, M. Taher Basir, Rusliza RS192 Materia Medica-Pharmaceutical Technology Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities in CCl4-induced hepatotoxic rat. Materials and methods: Sprague Dawley rats (n 1⁄4 6) were treated (p.o.) with 10% DMSO (Groups 1 and 2), 50mg/kg N-acetylcysteine (Group 3) or, 50, 250, or 500mg/kg MMCL (Groups 4–6) for 7 consecutive days followed by pretreatment (i.p.) with vehicle (Group 1) or 50% CCl4 in olive oil (v/v) (Groups 2–6) on day 7th. Plasma liver enzymes and hepatic antioxidant enzymes and pro-inflammatory cytokines concen- trations were measured while liver histopathology was examined. Results: MMCL, at 500 mg/kg, significantly (p < 0.05) ameliorated CCl4-induced hepatotoxicity by decreas- ing the plasma level of alanine transaminase (429.1 versus 168.7U/L) and aspartate transaminase (513.8 versus 438.1 U/L) as well as the tissue level of nitric oxide (62.7 versus 24.1 nmol/g tissue). At 50, 250, or 500mg/kg, MMCL significantly (p<0.05) reduced the tumour necrosis factor a (87.8 versus 32.7pg/mg tissue), interleukin-1b (1474.4 versus 618.3pg/mg tissue), and interleukin-6 (136.7 versus 30.8pg/mg tis- sue) while increased the liver catalase (92.1 versus 114.4 U/g tissue) and superoxide dismutase (3.4 versus 5.5 U/g tissue). Additionally, qualitative phytochemicals analysis showed that MMCL contained gallic acid, ferulic acid, quercetin, and genistein. Discussion and conclusions: MMCL ability to attenuate CCl4-induced hepatotoxicity could be helpful in the development of hepatoprotective agents with fewer side effects. Taylor and Francis 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72035/1/Pharmbiol_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4%20induced%20liver%20injury%20possible%20synergistic%20action%20of%20flavonoids%20and%20volatile%20bioactive.pdf application/pdf en http://irep.iium.edu.my/72035/7/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4_scopus.pdf Zakaria, Zainul Amiruddin and Mahmood, Nor Diyana and Omar, Maizatul Hasyima and Bakhtiar, M. Taher and Basir, Rusliza (2019) Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system. Pharmaceutical Biology, 57 (1). pp. 335-344. ISSN 1388-0209 E-ISSN 1744-5116 https://www.tandfonline.com/doi/abs/10.1080/13880209.2019.1606836 10.1080/13880209.2019.1606836
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Zakaria, Zainul Amiruddin Mahmood, Nor Diyana Omar, Maizatul Hasyima Bakhtiar, M. Taher Basir, Rusliza Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
description	Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities in CCl4-induced hepatotoxic rat. Materials and methods: Sprague Dawley rats (n 1⁄4 6) were treated (p.o.) with 10% DMSO (Groups 1 and 2), 50mg/kg N-acetylcysteine (Group 3) or, 50, 250, or 500mg/kg MMCL (Groups 4–6) for 7 consecutive days followed by pretreatment (i.p.) with vehicle (Group 1) or 50% CCl4 in olive oil (v/v) (Groups 2–6) on day 7th. Plasma liver enzymes and hepatic antioxidant enzymes and pro-inflammatory cytokines concen- trations were measured while liver histopathology was examined. Results: MMCL, at 500 mg/kg, significantly (p < 0.05) ameliorated CCl4-induced hepatotoxicity by decreas- ing the plasma level of alanine transaminase (429.1 versus 168.7U/L) and aspartate transaminase (513.8 versus 438.1 U/L) as well as the tissue level of nitric oxide (62.7 versus 24.1 nmol/g tissue). At 50, 250, or 500mg/kg, MMCL significantly (p<0.05) reduced the tumour necrosis factor a (87.8 versus 32.7pg/mg tissue), interleukin-1b (1474.4 versus 618.3pg/mg tissue), and interleukin-6 (136.7 versus 30.8pg/mg tis- sue) while increased the liver catalase (92.1 versus 114.4 U/g tissue) and superoxide dismutase (3.4 versus 5.5 U/g tissue). Additionally, qualitative phytochemicals analysis showed that MMCL contained gallic acid, ferulic acid, quercetin, and genistein. Discussion and conclusions: MMCL ability to attenuate CCl4-induced hepatotoxicity could be helpful in the development of hepatoprotective agents with fewer side effects.
format	Article
author	Zakaria, Zainul Amiruddin Mahmood, Nor Diyana Omar, Maizatul Hasyima Bakhtiar, M. Taher Basir, Rusliza
author_facet	Zakaria, Zainul Amiruddin Mahmood, Nor Diyana Omar, Maizatul Hasyima Bakhtiar, M. Taher Basir, Rusliza
author_sort	Zakaria, Zainul Amiruddin
title	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_short	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_full	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_fullStr	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_full_unstemmed	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_sort	methanol extract of muntingia calabura leaves attenuates ccl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
publisher	Taylor and Francis
publishDate	2019
url	http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/1/Pharmbiol_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4%20induced%20liver%20injury%20possible%20synergistic%20action%20of%20flavonoids%20and%20volatile%20bioactive.pdf http://irep.iium.edu.my/72035/7/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4_scopus.pdf
first_indexed	2023-09-18T21:42:08Z
last_indexed	2023-09-18T21:42:08Z
_version_	1777413210711785472

Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system

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