In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line

Background: Type 2 diabetes mellitus is a chronic metabolic disorder that is associated with mortality and morbidity. Recently, several plant-based agents have been used in the management of diabetes. Pluchea indica has been traditionally consumed as a medicinal plant in Southeast Asia, and its leav...

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Main Authors:	Suriyah, Wastuti Hidayati, Ichwan, Solachuddin J. A., Kasmuri, Abdul Razak, Taher, Muhammad
Format:	Article
Language:	English English
Published:	Universitas Indonesia 2019
Subjects:	RM Therapeutics. Pharmacology RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/71834/ http://irep.iium.edu.my/71834/ http://irep.iium.edu.my/71834/ http://irep.iium.edu.my/71834/1/71834_In%20vitro%20Evaluation%20of%20the%20Effect%20of%20Pluchea.pdf http://irep.iium.edu.my/71834/7/71834_In%20vitro%20Evaluation%20of%20the%20Effect%20of%20Pluchea%20indica%20Extracts%20in%20Promoting%20Glucose%20Consumption%20Activity%20on%20A%20Liver%20Cell%20Line_WOS.pdf

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spelling	iium-718342019-07-31T07:53:04Z http://irep.iium.edu.my/71834/ In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line Suriyah, Wastuti Hidayati Ichwan, Solachuddin J. A. Kasmuri, Abdul Razak Taher, Muhammad RM Therapeutics. Pharmacology RM300 Drugs and their action Background: Type 2 diabetes mellitus is a chronic metabolic disorder that is associated with mortality and morbidity. Recently, several plant-based agents have been used in the management of diabetes. Pluchea indica has been traditionally consumed as a medicinal plant in Southeast Asia, and its leaves have demonstrated induction of hypoglycemic effect in normal rats. This in vitro study aimed to evaluate the potency of P. indica extracts in stimulating glucose consumption in human liver CCL-13 cell line model. Methods: P. indica leaves were dried and extracted using a series of organic solvents and water. The effect of the extracts on cell viability was determined by MTT assay. The glucose consumption was analyzed using glucose oxidase method. Results: Our results revealed that the methanol extract of P. indica could significantly increase glucose consumption of cells in a concentration-dependent manner, which suggests the usefulness of the extract as an antidiabetic candidate via stimulation of glucose uptake into the liver cells. Conclusion: Our study suggests that P. indica is a potential natural candidate for diabetes mellitus management. Universitas Indonesia 2019-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/71834/1/71834_In%20vitro%20Evaluation%20of%20the%20Effect%20of%20Pluchea.pdf application/pdf en http://irep.iium.edu.my/71834/7/71834_In%20vitro%20Evaluation%20of%20the%20Effect%20of%20Pluchea%20indica%20Extracts%20in%20Promoting%20Glucose%20Consumption%20Activity%20on%20A%20Liver%20Cell%20Line_WOS.pdf Suriyah, Wastuti Hidayati and Ichwan, Solachuddin J. A. and Kasmuri, Abdul Razak and Taher, Muhammad (2019) In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line. Makara Journal of Health Research, 23 (1). pp. 48-52. ISSN 2356-3664 E-ISSN 2356-3656 http://journal.ui.ac.id/index.php/health/article/view/10153/pdf 10.7454/msk.v23i1.10153
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology RM300 Drugs and their action
spellingShingle	RM Therapeutics. Pharmacology RM300 Drugs and their action Suriyah, Wastuti Hidayati Ichwan, Solachuddin J. A. Kasmuri, Abdul Razak Taher, Muhammad In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line
description	Background: Type 2 diabetes mellitus is a chronic metabolic disorder that is associated with mortality and morbidity. Recently, several plant-based agents have been used in the management of diabetes. Pluchea indica has been traditionally consumed as a medicinal plant in Southeast Asia, and its leaves have demonstrated induction of hypoglycemic effect in normal rats. This in vitro study aimed to evaluate the potency of P. indica extracts in stimulating glucose consumption in human liver CCL-13 cell line model. Methods: P. indica leaves were dried and extracted using a series of organic solvents and water. The effect of the extracts on cell viability was determined by MTT assay. The glucose consumption was analyzed using glucose oxidase method. Results: Our results revealed that the methanol extract of P. indica could significantly increase glucose consumption of cells in a concentration-dependent manner, which suggests the usefulness of the extract as an antidiabetic candidate via stimulation of glucose uptake into the liver cells. Conclusion: Our study suggests that P. indica is a potential natural candidate for diabetes mellitus management.
format	Article
author	Suriyah, Wastuti Hidayati Ichwan, Solachuddin J. A. Kasmuri, Abdul Razak Taher, Muhammad
author_facet	Suriyah, Wastuti Hidayati Ichwan, Solachuddin J. A. Kasmuri, Abdul Razak Taher, Muhammad
author_sort	Suriyah, Wastuti Hidayati
title	In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line
title_short	In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line
title_full	In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line
title_fullStr	In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line
title_full_unstemmed	In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line
title_sort	in vitro evaluation of the effect of pluchea indica extracts in promoting glucose consumption activity on a liver cell line
publisher	Universitas Indonesia
publishDate	2019
url	http://irep.iium.edu.my/71834/ http://irep.iium.edu.my/71834/ http://irep.iium.edu.my/71834/ http://irep.iium.edu.my/71834/1/71834_In%20vitro%20Evaluation%20of%20the%20Effect%20of%20Pluchea.pdf http://irep.iium.edu.my/71834/7/71834_In%20vitro%20Evaluation%20of%20the%20Effect%20of%20Pluchea%20indica%20Extracts%20in%20Promoting%20Glucose%20Consumption%20Activity%20on%20A%20Liver%20Cell%20Line_WOS.pdf
first_indexed	2023-09-18T21:41:51Z
last_indexed	2023-09-18T21:41:51Z
_version_	1777413192304033792

In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line

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