Optimisation of browning index of Maillard reaction in gelatine powder by response surface methodology (RSM) for halal authentication

Gelatine as the product of collagen extraction from animals is widely used in the food industry. In a glance, the physical properties of gelatine from several sources such as fish, bovine and porcine are similar. Therefore, distinguishing between the sources of gelatine is a tedious task. The differ...

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Bibliographic Details
Main Authors: Hamid, Anis Hamizah, Elgharbawy, Amal A.M., Rohman, Abdul, Othman, Rashidi, A. Monsur, Hammed Ademola, Ahmad Fadzlillah, Nurrulhidayah
Format: Article
Language:English
English
English
Published: Rynnye Lyan Resources 2019
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Online Access:http://irep.iium.edu.my/71631/
http://irep.iium.edu.my/71631/
http://irep.iium.edu.my/71631/
http://irep.iium.edu.my/71631/1/71631_Optimisation%20of%20browning%20index%20of%20Maillard.pdf
http://irep.iium.edu.my/71631/7/71631_Optimisation%20of%20browning%20index%20of%20Maillard%20reaction_googlescholar.pdf
http://irep.iium.edu.my/71631/13/71631_Optimisation%20of%20browning%20index%20of%20maillard%20reaction_scopus.pdf
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Summary:Gelatine as the product of collagen extraction from animals is widely used in the food industry. In a glance, the physical properties of gelatine from several sources such as fish, bovine and porcine are similar. Therefore, distinguishing between the sources of gelatine is a tedious task. The differentiation of the gelatine from its sources requires an approach of a chemical reaction. This paper focused on the optimisation of Maillard reaction from different sources of gelatine by Response surface methodology (RSM). The experiment was designed with several imperative parameters; temperature, time and presence of metal ion Cu2+. The response was recorded from the absorbance of reacted gelatine mixture at specific wavenumber (420 nm) through UV-Vis instrumentation. The optimal reaction condition of all type of gelatines in water bath was 95°C for 9 hrs. From solution given, only 5mM concentration of metal ion Cu2+ has an influence on the bovine gelatine compared to fish and porcine gelatine. Maillard reaction with a combination of UV-Vis spectroscopy is one of the convenient protocols for rapid authentication purpose. RSM help to optimize the reaction condition of gelatine from different sources.