Non-alcoholic steatohepatitis animal model induction

Aim: The aims of this study are to establish NASH animal model utilizing 1% and 12% cholesterol diet. Methods: This study begins with the induction phase with cholesterol diet. In the induction phase, 42 Sprague-Dawley rats were randomly divided into standard diet (std) group (n=12) and NASH group (...

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Main Authors:	Abdul Rahim, Roslina, Abdul Ghani, Azril Shahreez, Muhammad, Naznin, Abdullah, Nor Zamzila, Ramli, Norazsida
Format:	Article
Language:	English
Published:	World Journal of Pharmacy and Pharmaceutical Sciences (WJPSS) 2019
Subjects:	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology
Online Access:	http://irep.iium.edu.my/71485/ http://irep.iium.edu.my/71485/ http://irep.iium.edu.my/71485/1/71485_Non-alcoholic%20steatohepatitis%20animal.pdf

id	iium-71485
recordtype	eprints
spelling	iium-714852019-04-05T03:20:02Z http://irep.iium.edu.my/71485/ Non-alcoholic steatohepatitis animal model induction Abdul Rahim, Roslina Abdul Ghani, Azril Shahreez Muhammad, Naznin Abdullah, Nor Zamzila Ramli, Norazsida QP Physiology R Medicine (General) RB Pathology RC Internal medicine RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology Aim: The aims of this study are to establish NASH animal model utilizing 1% and 12% cholesterol diet. Methods: This study begins with the induction phase with cholesterol diet. In the induction phase, 42 Sprague-Dawley rats were randomly divided into standard diet (std) group (n=12) and NASH group (n=30). The 12 rats in the std group were fed with commercial rat pellet while the 30 rats in the NASH group were fed with 1% cholesterol (1% CD) diet for 14 weeks. The experiment was extended for an additional 6 weeks with increment of cholesterol in the diet from 1% to 12 % in the NASH group since the liver histology did not show features of NASH in the 1% CD. Results: At the end of the additional 6 weeks period, liver histology showed NASH changes in the 12% CD. Results of the histological sections of the livers of the 12% CD rats in the induction phase exhibited features consistent with NASH which include macrovesicular steatosis, occasional hepatocyte ballooning degeneration with mild to moderate lobular and portal inflammation. The 12% CD group revealed significantly increased serum alanine aminotransferase (ALT), serum insulin levels, homeostatic model assessment insulin resistance (HOMA-IR), serum total cholesterol (TC), serum low-density lipoprotein cholesterol (LDL-c) and reduced high-density lipoprotein cholesterol (HDL-c) in rats. Conclusion: This study has successfully induced mild–moderate NASH rat model utilizing 12% cholesterol diet. World Journal of Pharmacy and Pharmaceutical Sciences (WJPSS) 2019-04-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/71485/1/71485_Non-alcoholic%20steatohepatitis%20animal.pdf Abdul Rahim, Roslina and Abdul Ghani, Azril Shahreez and Muhammad, Naznin and Abdullah, Nor Zamzila and Ramli, Norazsida (2019) Non-alcoholic steatohepatitis animal model induction. World Journal of Pharmacy and Pharmaceutical Sciences, 8 (4). pp. 1523-1538. ISSN 2278-4357 http://www.wjpps.com/wjpps_controller/current_issues
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology
spellingShingle	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology Abdul Rahim, Roslina Abdul Ghani, Azril Shahreez Muhammad, Naznin Abdullah, Nor Zamzila Ramli, Norazsida Non-alcoholic steatohepatitis animal model induction
description	Aim: The aims of this study are to establish NASH animal model utilizing 1% and 12% cholesterol diet. Methods: This study begins with the induction phase with cholesterol diet. In the induction phase, 42 Sprague-Dawley rats were randomly divided into standard diet (std) group (n=12) and NASH group (n=30). The 12 rats in the std group were fed with commercial rat pellet while the 30 rats in the NASH group were fed with 1% cholesterol (1% CD) diet for 14 weeks. The experiment was extended for an additional 6 weeks with increment of cholesterol in the diet from 1% to 12 % in the NASH group since the liver histology did not show features of NASH in the 1% CD. Results: At the end of the additional 6 weeks period, liver histology showed NASH changes in the 12% CD. Results of the histological sections of the livers of the 12% CD rats in the induction phase exhibited features consistent with NASH which include macrovesicular steatosis, occasional hepatocyte ballooning degeneration with mild to moderate lobular and portal inflammation. The 12% CD group revealed significantly increased serum alanine aminotransferase (ALT), serum insulin levels, homeostatic model assessment insulin resistance (HOMA-IR), serum total cholesterol (TC), serum low-density lipoprotein cholesterol (LDL-c) and reduced high-density lipoprotein cholesterol (HDL-c) in rats. Conclusion: This study has successfully induced mild–moderate NASH rat model utilizing 12% cholesterol diet.
format	Article
author	Abdul Rahim, Roslina Abdul Ghani, Azril Shahreez Muhammad, Naznin Abdullah, Nor Zamzila Ramli, Norazsida
author_facet	Abdul Rahim, Roslina Abdul Ghani, Azril Shahreez Muhammad, Naznin Abdullah, Nor Zamzila Ramli, Norazsida
author_sort	Abdul Rahim, Roslina
title	Non-alcoholic steatohepatitis animal model induction
title_short	Non-alcoholic steatohepatitis animal model induction
title_full	Non-alcoholic steatohepatitis animal model induction
title_fullStr	Non-alcoholic steatohepatitis animal model induction
title_full_unstemmed	Non-alcoholic steatohepatitis animal model induction
title_sort	non-alcoholic steatohepatitis animal model induction
publisher	World Journal of Pharmacy and Pharmaceutical Sciences (WJPSS)
publishDate	2019
url	http://irep.iium.edu.my/71485/ http://irep.iium.edu.my/71485/ http://irep.iium.edu.my/71485/1/71485_Non-alcoholic%20steatohepatitis%20animal.pdf
first_indexed	2023-09-18T21:41:22Z
last_indexed	2023-09-18T21:41:22Z
_version_	1777413162103996416

Non-alcoholic steatohepatitis animal model induction

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