Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology

The purpose of this research is to optimize the functionalization process of magnetic iron oxide nanoparticles for CLEA immobilization to enzymes, as a solution to the present challenges of CLEA technology such as clumping. A co-precipitation method was used to synthesize the magnetic nanoparticle...

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Main Authors:	Khatun, Hanufa, Yusof, Faridah, Mohamed Azmin, Nor Fadhillah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2018
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/71471/ http://irep.iium.edu.my/71471/ http://irep.iium.edu.my/71471/1/71471_Optimization%20of%20the%20Amino%20Functionalization.pdf

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recordtype	eprints
spelling	iium-714712019-07-16T18:09:58Z http://irep.iium.edu.my/71471/ Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology Khatun, Hanufa Yusof, Faridah Mohamed Azmin, Nor Fadhillah TP248.13 Biotechnology The purpose of this research is to optimize the functionalization process of magnetic iron oxide nanoparticles for CLEA immobilization to enzymes, as a solution to the present challenges of CLEA technology such as clumping. A co-precipitation method was used to synthesize the magnetic nanoparticles (MNP), in which ferric chloride (FeCl3.6H2O) and ferrous sulfate (FeSO4.7H2O) were used to provide Fe3+ and Fe2+ ions. Ammonium hydroxide (NH4OH) was used as the precipitator of the MNPs. For the MNPs to be immobilized with proteins through cross-linking, they had to be functionalized with amino groups by 3-aminopropyltrimetoxysilane (APTES), through a silanization reaction. Silicon ions in APTES connected with oxygen in the iron oxide particles, thus forming a connection between the nanoparticles and the amino groups inside APTES. The functionalization of the MNPs with amino groups was optimized using One Factor at a Time (OFAT), with APTES as the significant parameter. The percentage of BSA immobilized on the functionalized nanoparticles was used to determine the ability of the functionalized MNPs to immobilize proteins for each possible optimal run. The optimum concentration for APTES in the amino functionalization of magnetic nanoparticles was found to be 2.145 M. Kulliyyah of Engineering, International Islamic University Malaysia 2018-09-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/71471/1/71471_Optimization%20of%20the%20Amino%20Functionalization.pdf Khatun, Hanufa and Yusof, Faridah and Mohamed Azmin, Nor Fadhillah (2018) Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology. In: 5th International Conference on Biotechnology Engineering (ICBioE 2018), 19th-20th September 2018, Kuala Lumpur. http://www.iium.edu.my/icbioe/2018/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Khatun, Hanufa Yusof, Faridah Mohamed Azmin, Nor Fadhillah Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology
description	The purpose of this research is to optimize the functionalization process of magnetic iron oxide nanoparticles for CLEA immobilization to enzymes, as a solution to the present challenges of CLEA technology such as clumping. A co-precipitation method was used to synthesize the magnetic nanoparticles (MNP), in which ferric chloride (FeCl3.6H2O) and ferrous sulfate (FeSO4.7H2O) were used to provide Fe3+ and Fe2+ ions. Ammonium hydroxide (NH4OH) was used as the precipitator of the MNPs. For the MNPs to be immobilized with proteins through cross-linking, they had to be functionalized with amino groups by 3-aminopropyltrimetoxysilane (APTES), through a silanization reaction. Silicon ions in APTES connected with oxygen in the iron oxide particles, thus forming a connection between the nanoparticles and the amino groups inside APTES. The functionalization of the MNPs with amino groups was optimized using One Factor at a Time (OFAT), with APTES as the significant parameter. The percentage of BSA immobilized on the functionalized nanoparticles was used to determine the ability of the functionalized MNPs to immobilize proteins for each possible optimal run. The optimum concentration for APTES in the amino functionalization of magnetic nanoparticles was found to be 2.145 M.
format	Conference or Workshop Item
author	Khatun, Hanufa Yusof, Faridah Mohamed Azmin, Nor Fadhillah
author_facet	Khatun, Hanufa Yusof, Faridah Mohamed Azmin, Nor Fadhillah
author_sort	Khatun, Hanufa
title	Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology
title_short	Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology
title_full	Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology
title_fullStr	Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology
title_full_unstemmed	Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology
title_sort	optimization of the amino functionalization of magnetic nanoparticles for use in mnp-clea technology
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/71471/ http://irep.iium.edu.my/71471/ http://irep.iium.edu.my/71471/1/71471_Optimization%20of%20the%20Amino%20Functionalization.pdf
first_indexed	2023-09-18T21:41:21Z
last_indexed	2023-09-18T21:41:21Z
_version_	1777413160944271360

Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology

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