Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles

The purpose of this research is to optimize the immobilization of lipase from the viscera of channel catfish to magnetic nanoparticles (MNP-lipase) using CLEA technology, forming a new immobilized enzyme. Lipase was obtained from the viscera of channel catfish, using ammonium sulfate to salt it ou...

Full description

Bibliographic Details
Main Authors:	Khatun, Hanufa, Yusof, Faridah, Mohamed Azmin, Nor Fadhillah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2018
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/71470/ http://irep.iium.edu.my/71470/ http://irep.iium.edu.my/71470/1/71470_Synthesis%20and%20Process%20Parameter%20Optimization.pdf

id	iium-71470
recordtype	eprints
spelling	iium-714702019-04-05T06:54:19Z http://irep.iium.edu.my/71470/ Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles Khatun, Hanufa Yusof, Faridah Mohamed Azmin, Nor Fadhillah TP248.13 Biotechnology The purpose of this research is to optimize the immobilization of lipase from the viscera of channel catfish to magnetic nanoparticles (MNP-lipase) using CLEA technology, forming a new immobilized enzyme. Lipase was obtained from the viscera of channel catfish, using ammonium sulfate to salt it out. Magnetic nanoparticles were obtained using the co-precipitation method, in which ferric chloride (FeCl3.6H2O) and ferrous sulfate (FeSO4.7H2O) gave the Fe3+ and Fe2+ ions, and precipitation was done by adding ammonium hydroxide which enables the formation of iron oxide magnetic nanoparticles. The functionalized magnetic nanoparticles were immobilized to lipase using the CLEA method, and this immobilization process was optimized. Glutaraldehyde was used as the cross-linker, bovine serum albumin (BSA) was used as a proteic feeder, and acetone as the solvent. One Factor at a Time (OFAT) was used in the process of optimization. The significant factors were considered to be glutaraldehyde, acetone and BSA, and the optimum concentrations of these components were determined to be 80 mM, 60% v/v and 0.18 mM respectively, to produce an optimum new MNP-lipase from the viscera of channel catfish. Kulliyyah of Engineering, International Islamic University Malaysia 2018-09 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/71470/1/71470_Synthesis%20and%20Process%20Parameter%20Optimization.pdf Khatun, Hanufa and Yusof, Faridah and Mohamed Azmin, Nor Fadhillah (2018) Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles. In: 5th International Conference on Biotechnology Engineering (ICBioE 2018), 19th-20th September 2018, Kuala Lumpur. http://www.iium.edu.my/icbioe/2018/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Khatun, Hanufa Yusof, Faridah Mohamed Azmin, Nor Fadhillah Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles
description	The purpose of this research is to optimize the immobilization of lipase from the viscera of channel catfish to magnetic nanoparticles (MNP-lipase) using CLEA technology, forming a new immobilized enzyme. Lipase was obtained from the viscera of channel catfish, using ammonium sulfate to salt it out. Magnetic nanoparticles were obtained using the co-precipitation method, in which ferric chloride (FeCl3.6H2O) and ferrous sulfate (FeSO4.7H2O) gave the Fe3+ and Fe2+ ions, and precipitation was done by adding ammonium hydroxide which enables the formation of iron oxide magnetic nanoparticles. The functionalized magnetic nanoparticles were immobilized to lipase using the CLEA method, and this immobilization process was optimized. Glutaraldehyde was used as the cross-linker, bovine serum albumin (BSA) was used as a proteic feeder, and acetone as the solvent. One Factor at a Time (OFAT) was used in the process of optimization. The significant factors were considered to be glutaraldehyde, acetone and BSA, and the optimum concentrations of these components were determined to be 80 mM, 60% v/v and 0.18 mM respectively, to produce an optimum new MNP-lipase from the viscera of channel catfish.
format	Conference or Workshop Item
author	Khatun, Hanufa Yusof, Faridah Mohamed Azmin, Nor Fadhillah
author_facet	Khatun, Hanufa Yusof, Faridah Mohamed Azmin, Nor Fadhillah
author_sort	Khatun, Hanufa
title	Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles
title_short	Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles
title_full	Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles
title_fullStr	Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles
title_full_unstemmed	Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles
title_sort	synthesis and process parameter optimization of immobilizing clea-lipase from fish viscera onto magnetic nanoparticles
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/71470/ http://irep.iium.edu.my/71470/ http://irep.iium.edu.my/71470/1/71470_Synthesis%20and%20Process%20Parameter%20Optimization.pdf
first_indexed	2023-09-18T21:41:21Z
last_indexed	2023-09-18T21:41:21Z
_version_	1777413160800616448

Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles

Similar Items