Synthesis and process parameter optimization of immobilizing CLEA-lipase from fish viscera onto magnetic nanoparticles
The purpose of this research is to optimize the immobilization of lipase from the viscera of channel catfish to magnetic nanoparticles (MNP-lipase) using CLEA technology, forming a new immobilized enzyme. Lipase was obtained from the viscera of channel catfish, using ammonium sulfate to salt it ou...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyyah of Engineering, International Islamic University Malaysia
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/71470/ http://irep.iium.edu.my/71470/ http://irep.iium.edu.my/71470/1/71470_Synthesis%20and%20Process%20Parameter%20Optimization.pdf |
| Summary: | The purpose of this research is to optimize the immobilization of lipase from the viscera of channel catfish to
magnetic nanoparticles (MNP-lipase) using CLEA technology, forming a new immobilized enzyme. Lipase was
obtained from the viscera of channel catfish, using ammonium sulfate to salt it out. Magnetic nanoparticles
were obtained using the co-precipitation method, in which ferric chloride (FeCl3.6H2O) and ferrous sulfate
(FeSO4.7H2O) gave the Fe3+ and Fe2+ ions, and precipitation was done by adding ammonium hydroxide which
enables the formation of iron oxide magnetic nanoparticles. The functionalized magnetic nanoparticles were
immobilized to lipase using the CLEA method, and this immobilization process was optimized. Glutaraldehyde
was used as the cross-linker, bovine serum albumin (BSA) was used as a proteic feeder, and acetone as the
solvent. One Factor at a Time (OFAT) was used in the process of optimization. The significant factors were
considered to be glutaraldehyde, acetone and BSA, and the optimum concentrations of these components were
determined to be 80 mM, 60% v/v and 0.18 mM respectively, to produce an optimum new MNP-lipase from
the viscera of channel catfish. |
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