Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum

Cancer is by far one of the most worrying health issues that continues to be the major killing diseases worldwide, accounting for more than 6 million deaths. It is predicted that cancer incidence is continue rising every year with an approximated 12 million of deaths by 2030. In the confrontation of...

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Main Authors:	Ghazali, Nor Farahiyah, Mustafa, Farah Farisha, Tengku Muhammad, Tengku Sifzizul, Ibrahim, Maizatul Akma
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	Q Science (General) QK Botany RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/71452/ http://irep.iium.edu.my/71452/ http://irep.iium.edu.my/71452/1/71452_Antioxidant%20activity%20and%20in%20vitro%20-%20abstract.pdf http://irep.iium.edu.my/71452/2/71452_Antioxidant%20activity%20and%20in%20vitro%20-%20paper.pdf

id	iium-71452
recordtype	eprints
spelling	iium-714522019-04-03T01:25:05Z http://irep.iium.edu.my/71452/ Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum Ghazali, Nor Farahiyah Mustafa, Farah Farisha Tengku Muhammad, Tengku Sifzizul Ibrahim, Maizatul Akma Q Science (General) QK Botany RM Therapeutics. Pharmacology RS Pharmacy and materia medica Cancer is by far one of the most worrying health issues that continues to be the major killing diseases worldwide, accounting for more than 6 million deaths. It is predicted that cancer incidence is continue rising every year with an approximated 12 million of deaths by 2030. In the confrontation of advances in modern medicine such as surgery, chemotherapy, radiotherapy and hormone therapy, cancer disease persists a worldwide problem. Thus the search for excellent anticancer agents avertable to human malignancies is needed from the nature. The winnowing of natural products notably plant extracts in few exceptional clinically active plant products such as vinca alkaloids and podophyllotoxin derivatives. Since the past few years, the search for new alternatives has been concentrated in finding medicinal herbs from local natural resources. In fact, about 60% of currently applied cancer drugs are derived from natural sources, due to its fewer side effects. Piper sarmentosum Roxb. which is locally known as ‘kaduk’, is one of natural medicinal plant that had been used traditionally to treat headache, arthritis, menstrual pain, cough and eczema. It is proven to have various biological properties such as anti-inflammatory, antioxidant, antimalarial, antiplasmodial, anti-diabetic, antifungal and anticarcinogenic. The aims of this study were to identify the phenolic compounds of P. sarmentosum methanolic extracts by measuring total phenolic content, and HPLC analysis. The antioxidant activity was also evaluated by DPPH scavenging assay. MTS assay was also conducted to study the effect of the extracts on human breast cancer cells. The HPLC analysis showed the presence of quercetin, naringin, gallic acid and tannic acid in P. sarmentosum. P. sarmentosum exhibited antioxidant property at 96.98 ± 2.29 μg/mL by DPPH scavenging activity with its high phenolic content at 89.22 mg GAE/ g dry extract. The cytotoxicity screening of P. sarmentosum extract using MTS assay provided IC50 of 24.63 ± 0.23 μg/mL and 2.85 ± 0.16 μg/mL on MCF-7 and T-47D, respectively. In conclusion, the phenolic compounds obtained from P. sarmentosum possess potent antioxidant and anticancer activity against the tested breast cancer cells. Further study might focus on the compounds action mechanisms toward the cancerous cells. 2018-08-17 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/71452/1/71452_Antioxidant%20activity%20and%20in%20vitro%20-%20abstract.pdf application/pdf en http://irep.iium.edu.my/71452/2/71452_Antioxidant%20activity%20and%20in%20vitro%20-%20paper.pdf Ghazali, Nor Farahiyah and Mustafa, Farah Farisha and Tengku Muhammad, Tengku Sifzizul and Ibrahim, Maizatul Akma (2018) Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum. In: International Conference of Analytical Sciences (SKAM31), 17th-19th August 2018, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/skam31/wp-content/uploads/2018/08/SKAM31-PROGRAMME-BOOK-PART-1.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General) QK Botany RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	Q Science (General) QK Botany RM Therapeutics. Pharmacology RS Pharmacy and materia medica Ghazali, Nor Farahiyah Mustafa, Farah Farisha Tengku Muhammad, Tengku Sifzizul Ibrahim, Maizatul Akma Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum
description	Cancer is by far one of the most worrying health issues that continues to be the major killing diseases worldwide, accounting for more than 6 million deaths. It is predicted that cancer incidence is continue rising every year with an approximated 12 million of deaths by 2030. In the confrontation of advances in modern medicine such as surgery, chemotherapy, radiotherapy and hormone therapy, cancer disease persists a worldwide problem. Thus the search for excellent anticancer agents avertable to human malignancies is needed from the nature. The winnowing of natural products notably plant extracts in few exceptional clinically active plant products such as vinca alkaloids and podophyllotoxin derivatives. Since the past few years, the search for new alternatives has been concentrated in finding medicinal herbs from local natural resources. In fact, about 60% of currently applied cancer drugs are derived from natural sources, due to its fewer side effects. Piper sarmentosum Roxb. which is locally known as ‘kaduk’, is one of natural medicinal plant that had been used traditionally to treat headache, arthritis, menstrual pain, cough and eczema. It is proven to have various biological properties such as anti-inflammatory, antioxidant, antimalarial, antiplasmodial, anti-diabetic, antifungal and anticarcinogenic. The aims of this study were to identify the phenolic compounds of P. sarmentosum methanolic extracts by measuring total phenolic content, and HPLC analysis. The antioxidant activity was also evaluated by DPPH scavenging assay. MTS assay was also conducted to study the effect of the extracts on human breast cancer cells. The HPLC analysis showed the presence of quercetin, naringin, gallic acid and tannic acid in P. sarmentosum. P. sarmentosum exhibited antioxidant property at 96.98 ± 2.29 μg/mL by DPPH scavenging activity with its high phenolic content at 89.22 mg GAE/ g dry extract. The cytotoxicity screening of P. sarmentosum extract using MTS assay provided IC50 of 24.63 ± 0.23 μg/mL and 2.85 ± 0.16 μg/mL on MCF-7 and T-47D, respectively. In conclusion, the phenolic compounds obtained from P. sarmentosum possess potent antioxidant and anticancer activity against the tested breast cancer cells. Further study might focus on the compounds action mechanisms toward the cancerous cells.
format	Conference or Workshop Item
author	Ghazali, Nor Farahiyah Mustafa, Farah Farisha Tengku Muhammad, Tengku Sifzizul Ibrahim, Maizatul Akma
author_facet	Ghazali, Nor Farahiyah Mustafa, Farah Farisha Tengku Muhammad, Tengku Sifzizul Ibrahim, Maizatul Akma
author_sort	Ghazali, Nor Farahiyah
title	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum
title_short	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum
title_full	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum
title_fullStr	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum
title_full_unstemmed	Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum
title_sort	antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from piper sarmentosum
publishDate	2018
url	http://irep.iium.edu.my/71452/ http://irep.iium.edu.my/71452/ http://irep.iium.edu.my/71452/1/71452_Antioxidant%20activity%20and%20in%20vitro%20-%20abstract.pdf http://irep.iium.edu.my/71452/2/71452_Antioxidant%20activity%20and%20in%20vitro%20-%20paper.pdf
first_indexed	2023-09-18T21:41:19Z
last_indexed	2023-09-18T21:41:19Z
_version_	1777413159347290112

Antioxidant activity and in vitro cytotoxicity study of the phenolic compounds from Piper sarmentosum

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