Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells

Two newly-synthesized amino acids thiourea derivatives; 2-(3-benzoylthioureido)-3-mercaptopropanoic acid and 2-(3-benzoylthioureido)-4-(methylthio)butanoic acid, which were labeled as M1 and M2 respectively, were evaluated for their potential as anti-amoebic agent, aiming for a new discovery in amoe...

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Main Authors:	Ibrahim, Maizatul Akma, Nordin, Mohd Shukor, Mat Amin, Nakisah, Mohd Yusof, Mohd Sukeri
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	Q Science (General) QD Chemistry RE Ophthalmology
Online Access:	http://irep.iium.edu.my/71450/ http://irep.iium.edu.my/71450/1/Maizatul%20Akma%20Ibrahim-ACB2015.pdf http://irep.iium.edu.my/71450/2/71450%20-abstract.pdf

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recordtype	eprints
spelling	iium-714502019-04-02T07:07:58Z http://irep.iium.edu.my/71450/ Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells Ibrahim, Maizatul Akma Nordin, Mohd Shukor Mat Amin, Nakisah Mohd Yusof, Mohd Sukeri Q Science (General) QD Chemistry RE Ophthalmology Two newly-synthesized amino acids thiourea derivatives; 2-(3-benzoylthioureido)-3-mercaptopropanoic acid and 2-(3-benzoylthioureido)-4-(methylthio)butanoic acid, which were labeled as M1 and M2 respectively, were evaluated for their potential as anti-amoebic agent, aiming for a new discovery in amoebic keratitis treatment. The compounds were tested on Acanthamoeba castellanii (CCAP 1501/2A) and Acanthamoeba sp. (Hospital Kuala Lumpur isolate), and also on human corneal epithelial cells (HCEC). Experiments conducted consisting of IC50 determination by eosin dye and MTT assay, morphological observation by light microscopy, evaluation of membrane integrity by acridine orange/propidium iodide staining, mode of cell death determination by DNA fragmentation test and assessment of DNA damage by alkaline comet assay. The IC50 obtained for M1 were 6.26 μM for A. castellanii, and 9.00 μM for Acanthamoeba sp. (HKL isolate) while for M2 the values were 6.97 and 8.63 μM respectively, indicating that these compounds are cytotoxic against both Acanthamoeba. They shortened acanthopodia structures, transformed the amoeba cells to become rounded, and exhibited no distinct vacuoles and nucleus. The membrane integrity was also disrupted, making them non-intact, and promoted apoptosis in amoeba but did not significantly affected the DNA. Both thiourea derivatives showed moderate cytotoxicity toward HCEC with IC50 at 132.69 and 98.20 μM respectively. The compounds did not significantly alter corneal cells’ cellular morphology. These derivatives were found to disrupt HCEC’s membrane integrity and promoted apoptosis but non-genotoxic on HCEC’s DNA. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/71450/1/Maizatul%20Akma%20Ibrahim-ACB2015.pdf application/pdf en http://irep.iium.edu.my/71450/2/71450%20-abstract.pdf Ibrahim, Maizatul Akma and Nordin, Mohd Shukor and Mat Amin, Nakisah and Mohd Yusof, Mohd Sukeri (2015) Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells. In: Asian Congress On Biotechnology 2015, 15th-19th November 2015, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General) QD Chemistry RE Ophthalmology
spellingShingle	Q Science (General) QD Chemistry RE Ophthalmology Ibrahim, Maizatul Akma Nordin, Mohd Shukor Mat Amin, Nakisah Mohd Yusof, Mohd Sukeri Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells
description	Two newly-synthesized amino acids thiourea derivatives; 2-(3-benzoylthioureido)-3-mercaptopropanoic acid and 2-(3-benzoylthioureido)-4-(methylthio)butanoic acid, which were labeled as M1 and M2 respectively, were evaluated for their potential as anti-amoebic agent, aiming for a new discovery in amoebic keratitis treatment. The compounds were tested on Acanthamoeba castellanii (CCAP 1501/2A) and Acanthamoeba sp. (Hospital Kuala Lumpur isolate), and also on human corneal epithelial cells (HCEC). Experiments conducted consisting of IC50 determination by eosin dye and MTT assay, morphological observation by light microscopy, evaluation of membrane integrity by acridine orange/propidium iodide staining, mode of cell death determination by DNA fragmentation test and assessment of DNA damage by alkaline comet assay. The IC50 obtained for M1 were 6.26 μM for A. castellanii, and 9.00 μM for Acanthamoeba sp. (HKL isolate) while for M2 the values were 6.97 and 8.63 μM respectively, indicating that these compounds are cytotoxic against both Acanthamoeba. They shortened acanthopodia structures, transformed the amoeba cells to become rounded, and exhibited no distinct vacuoles and nucleus. The membrane integrity was also disrupted, making them non-intact, and promoted apoptosis in amoeba but did not significantly affected the DNA. Both thiourea derivatives showed moderate cytotoxicity toward HCEC with IC50 at 132.69 and 98.20 μM respectively. The compounds did not significantly alter corneal cells’ cellular morphology. These derivatives were found to disrupt HCEC’s membrane integrity and promoted apoptosis but non-genotoxic on HCEC’s DNA.
format	Conference or Workshop Item
author	Ibrahim, Maizatul Akma Nordin, Mohd Shukor Mat Amin, Nakisah Mohd Yusof, Mohd Sukeri
author_facet	Ibrahim, Maizatul Akma Nordin, Mohd Shukor Mat Amin, Nakisah Mohd Yusof, Mohd Sukeri
author_sort	Ibrahim, Maizatul Akma
title	Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells
title_short	Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells
title_full	Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells
title_fullStr	Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells
title_full_unstemmed	Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells
title_sort	cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells
publishDate	2015
url	http://irep.iium.edu.my/71450/ http://irep.iium.edu.my/71450/1/Maizatul%20Akma%20Ibrahim-ACB2015.pdf http://irep.iium.edu.my/71450/2/71450%20-abstract.pdf
first_indexed	2023-09-18T21:41:19Z
last_indexed	2023-09-18T21:41:19Z
_version_	1777413159070466048

Cytotoxicity and genotoxicity studies of synthesized amino acid thiourea derivatives on acanthamoeba spp. and human corneal epithelial cells

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