Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques

The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids,...

Full description

Bibliographic Details
Main Authors:	Alara, Oluwaseun Ruth, Nour, Abdurahman Hamid, Ukaegbu, Chinonso Ishmael, Kabbashi, Nassereldeen Ahmed
Format:	Article
Language:	English English
Published:	Informa UK Limited, trading as Taylor & Francis Group 2019
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/71410/ http://irep.iium.edu.my/71410/ http://irep.iium.edu.my/71410/ http://irep.iium.edu.my/71410/1/71410_Extraction%20and%20characterization%20of%20bioactive.pdf http://irep.iium.edu.my/71410/2/71410_Extraction%20and%20characterization%20of%20bioactive_WOS.pdf

id	iium-71410
recordtype	eprints
spelling	iium-714102019-07-12T04:01:37Z http://irep.iium.edu.my/71410/ Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques Alara, Oluwaseun Ruth Nour, Abdurahman Hamid Ukaegbu, Chinonso Ishmael Kabbashi, Nassereldeen Ahmed TP155 Chemical engineering The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins, and the absence of anthraquinones. Furthermore, gas chromatography-mass spectroscopy (GCMS) analysis results revealed the presence of more phytochemicals in the extract obtained through MAE compared to Soxhlet extraction technique, whereby phytol was present in a higher percentage. Fourier transform infrared spectroscopy analysis confirmed the presence of phytochemicals in the extracts. Moreover, the IC50 values of the extracts antioxidant activities were evaluated using DPPH and ABTS assays. There exists a clear correlation between total phenolic content, total flavonoid content and antioxidant activity of the extracts. Therefore, this study suggested that higher phenolic compounds responsible for natural antioxidant could be obtained from V. amygdalina leaf using the microwave-assisted extraction technique. Informa UK Limited, trading as Taylor & Francis Group 2019-02-22 Article PeerReviewed application/pdf en http://irep.iium.edu.my/71410/1/71410_Extraction%20and%20characterization%20of%20bioactive.pdf application/pdf en http://irep.iium.edu.my/71410/2/71410_Extraction%20and%20characterization%20of%20bioactive_WOS.pdf Alara, Oluwaseun Ruth and Nour, Abdurahman Hamid and Ukaegbu, Chinonso Ishmael and Kabbashi, Nassereldeen Ahmed (2019) Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques. Journal of Taibah University for Science, 13 (1). pp. 414-422. ISSN 1658-3655 https://www.tandfonline.com/doi/full/10.1080/16583655.2019.1582460 10.1080/16583655.2019.1582460
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Alara, Oluwaseun Ruth Nour, Abdurahman Hamid Ukaegbu, Chinonso Ishmael Kabbashi, Nassereldeen Ahmed Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
description	The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins, and the absence of anthraquinones. Furthermore, gas chromatography-mass spectroscopy (GCMS) analysis results revealed the presence of more phytochemicals in the extract obtained through MAE compared to Soxhlet extraction technique, whereby phytol was present in a higher percentage. Fourier transform infrared spectroscopy analysis confirmed the presence of phytochemicals in the extracts. Moreover, the IC50 values of the extracts antioxidant activities were evaluated using DPPH and ABTS assays. There exists a clear correlation between total phenolic content, total flavonoid content and antioxidant activity of the extracts. Therefore, this study suggested that higher phenolic compounds responsible for natural antioxidant could be obtained from V. amygdalina leaf using the microwave-assisted extraction technique.
format	Article
author	Alara, Oluwaseun Ruth Nour, Abdurahman Hamid Ukaegbu, Chinonso Ishmael Kabbashi, Nassereldeen Ahmed
author_facet	Alara, Oluwaseun Ruth Nour, Abdurahman Hamid Ukaegbu, Chinonso Ishmael Kabbashi, Nassereldeen Ahmed
author_sort	Alara, Oluwaseun Ruth
title	Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_short	Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_full	Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_fullStr	Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_full_unstemmed	Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_sort	extraction and characterization of bioactive compounds in vernonia amygdalina leaf ethanolic extract comparing soxhlet and microwave-assisted extraction techniques
publisher	Informa UK Limited, trading as Taylor & Francis Group
publishDate	2019
url	http://irep.iium.edu.my/71410/ http://irep.iium.edu.my/71410/ http://irep.iium.edu.my/71410/ http://irep.iium.edu.my/71410/1/71410_Extraction%20and%20characterization%20of%20bioactive.pdf http://irep.iium.edu.my/71410/2/71410_Extraction%20and%20characterization%20of%20bioactive_WOS.pdf
first_indexed	2023-09-18T21:41:16Z
last_indexed	2023-09-18T21:41:16Z
_version_	1777413156343119872

Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques

Similar Items