Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM

Cytogenetic is the study of chromosomes at the cellular level. Only cells that are actively dividing can be used for cytogenetic studies. Although specific techniques differ according to the types of tissue culture, addition of a mitotic inhibitor to arrest cells at metaphase, harvesting of cells, s...

Full description

Bibliographic Details
Main Author:	Muhammad, Siti Aesah @ Naznin
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2009
Subjects:	L Education (General) LG Individual institutions (Asia. Africa) R Medicine (General)
Online Access:	http://irep.iium.edu.my/7139/ http://irep.iium.edu.my/7139/1/Establishment_of_Research_Methods_and_Protocols_in_Cytogenetic_Laboratory_of_the_Kulliyah_of_Medicine%2C_IIUM.pdf

id	iium-7139
recordtype	eprints
spelling	iium-71392011-11-30T08:35:07Z http://irep.iium.edu.my/7139/ Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM Muhammad, Siti Aesah @ Naznin L Education (General) LG Individual institutions (Asia. Africa) R Medicine (General) Cytogenetic is the study of chromosomes at the cellular level. Only cells that are actively dividing can be used for cytogenetic studies. Although specific techniques differ according to the types of tissue culture, addition of a mitotic inhibitor to arrest cells at metaphase, harvesting of cells, slide preparation and staining of the chromosomes. Since every cytogenetic laboratory has a different method of culturing samples, the aim of this study is to establish the method of lymphocyte and bone marrow cultures in our newly established cytogenetic laboratory of Kulliyyah of Medicine IIUM. Samples of peripheral blood and bone marrow were cultured using different culture techniques. Variations for each technique were instituted for the purpose of optimization. Different protocol for harvesting, slide making and banding were carried out to maximize the yield that is to obtain good quality of chromosomes for analysis. Fifty peripheral blood and 400 bone marrow aspiration cultures were set up. In the initial stages the yield of the cultures were poor. Some of the cultures were infected, mainly due to the prolonged incubation period. The harvesting technique was generally satisfactory though intermittently cytoplasmic problem and cell clumps were encountered. The banding methods used were Giemsa and Leishman banding. Generally for bone marrow cultures in addition to poor banding quality, the number of metaphase spreads obtained was poor and inadequate for analysis. Though the result obtained it was found that it has improved markedly, several stages of the methods need to be further optimized in order to obtain good number of metaphase spreads and also to improve the banding quality which will make it possible to analyze the chromosomes. IIUM Press 2009 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/7139/1/Establishment_of_Research_Methods_and_Protocols_in_Cytogenetic_Laboratory_of_the_Kulliyah_of_Medicine%2C_IIUM.pdf Muhammad, Siti Aesah @ Naznin (2009) Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM. In: Proceedings of the seminar on research findings 2008. IIUM Press, Kuala Lumpur, pp. 455-461. ISBN 9789833855780
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	L Education (General) LG Individual institutions (Asia. Africa) R Medicine (General)
spellingShingle	L Education (General) LG Individual institutions (Asia. Africa) R Medicine (General) Muhammad, Siti Aesah @ Naznin Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM
description	Cytogenetic is the study of chromosomes at the cellular level. Only cells that are actively dividing can be used for cytogenetic studies. Although specific techniques differ according to the types of tissue culture, addition of a mitotic inhibitor to arrest cells at metaphase, harvesting of cells, slide preparation and staining of the chromosomes. Since every cytogenetic laboratory has a different method of culturing samples, the aim of this study is to establish the method of lymphocyte and bone marrow cultures in our newly established cytogenetic laboratory of Kulliyyah of Medicine IIUM. Samples of peripheral blood and bone marrow were cultured using different culture techniques. Variations for each technique were instituted for the purpose of optimization. Different protocol for harvesting, slide making and banding were carried out to maximize the yield that is to obtain good quality of chromosomes for analysis. Fifty peripheral blood and 400 bone marrow aspiration cultures were set up. In the initial stages the yield of the cultures were poor. Some of the cultures were infected, mainly due to the prolonged incubation period. The harvesting technique was generally satisfactory though intermittently cytoplasmic problem and cell clumps were encountered. The banding methods used were Giemsa and Leishman banding. Generally for bone marrow cultures in addition to poor banding quality, the number of metaphase spreads obtained was poor and inadequate for analysis. Though the result obtained it was found that it has improved markedly, several stages of the methods need to be further optimized in order to obtain good number of metaphase spreads and also to improve the banding quality which will make it possible to analyze the chromosomes.
format	Book Chapter
author	Muhammad, Siti Aesah @ Naznin
author_facet	Muhammad, Siti Aesah @ Naznin
author_sort	Muhammad, Siti Aesah @ Naznin
title	Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM
title_short	Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM
title_full	Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM
title_fullStr	Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM
title_full_unstemmed	Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM
title_sort	establishment of research methods and protocols in cytogenetic laboratory of the kulliyah of medicine, iium
publisher	IIUM Press
publishDate	2009
url	http://irep.iium.edu.my/7139/ http://irep.iium.edu.my/7139/1/Establishment_of_Research_Methods_and_Protocols_in_Cytogenetic_Laboratory_of_the_Kulliyah_of_Medicine%2C_IIUM.pdf
first_indexed	2023-09-18T20:16:26Z
last_indexed	2023-09-18T20:16:26Z
_version_	1777407818248224768

Establishment of research methods and protocols in cytogenetic laboratory of the Kulliyah of Medicine, IIUM

Similar Items