Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile
Fucoxanthin has interesting anticancer activity, but is insoluble in water, hindering its use as a drug. Microencapsulation is used as a technique for improving drug delivery. This study aimed to formulate fucoxanthin-loaded microspheres (F-LM) for anticancer treatment of H1299 cancer cell lines and...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
MDPI
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/7/71146%20Optimization%20and%20Formulation.pdf http://irep.iium.edu.my/71146/8/71146%20Optimization%20and%20Formulation%20SCOPUS.pdf http://irep.iium.edu.my/71146/19/71146_Optimization%20and%20Formulation%20of%20Fucoxanthin-Loaded%20Microsphere%20%28F-LM%29%20Using%20Response%20Surface%20Methodology%20%28RSM%29%20and%20Analysis%20of%20Its%20Fucoxanthin%20Release%20Profile_wos.pdf |
| Summary: | Fucoxanthin has interesting anticancer activity, but is insoluble in water, hindering its use as a drug. Microencapsulation is used as a technique for improving drug delivery. This study aimed to formulate fucoxanthin-loaded microspheres (F-LM) for anticancer treatment of H1299 cancer cell lines and optimize particle size (PS) and encapsulation efficiency (EE). Using response surface methodology (RSM), a face centered central composite design (FCCCD) was designed with three factors: Polyvinylalcohol (PVA), poly(D,L-lactic-co-glycolic acid) (PLGA), and fucoxanthin concentration. F-LM was produced using a modified double-emulsion solvent evaporation method. The F-LM were characterized for release profile, release kinetics, and degradation pattern. Optimal F-LM PS and EE of 9.18 µm and 33.09%, respectively, with good surface morphology, were achieved from a 0.5% (w/v) PVA, 6.0% (w/v) PLGA, 200 µg/mL fucoxanthin formulation at a homogenization speed of 20,500 rpm. PVA concentration was the most significant factor (p < 0.05) affecting PS. Meanwhile, EE was significantly affected by interaction between the three factors: PVA, PLGA, and fucoxanthin. In vitro release curve showed fucoxanthin had a high burst release (38.3%) at the first hour, followed by a sustained release stage reaching (79.1%) within 2 months. Release kinetics followed a diffusion pattern predominantly controlled by the Higuchi model. Biodegradability studies based on surface morphology changes on the surface of the F-LM, show that morphology changed within the first hour, and F-LM completely degraded within 2 months. RSM under FCCCD design improved the difference between the lowest and highest responses, with good correlation between observed and predicted values for PS and EE of F-LM. |
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