Structural changes and the differential expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL) in subchondral bone during the development of osteoarthriti
Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as osteoprotegerin (OPG) and receptor activator of nuclea...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Malaysian Society of Applied Biology
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/71138/ http://irep.iium.edu.my/71138/ http://irep.iium.edu.my/71138/1/71138%20STRUCTURAL%20CHANGES%20AND%20THE%20DIFFERENTIAL.pdf http://irep.iium.edu.my/71138/3/71138%20Structural%20changes%20and%20the%20differential%20expression%20SCOPUS.pdf |
| Summary: | Osteoarthritis is a degenerative joint disease that is characterized by subchondral bone changes. These changes may be associated
with bone remodelling, which involve coordinative functions between bone formation and resorption markers such as
osteoprotegerin (OPG) and receptor activator of nuclear factor kB ligand (RANKL), respectively. Previous studies have
shown that the structural changes were different between the subchondral bone plate and trabecular bone. However, the
expression of bone formation and bone resorption markers underlying the differences between these two regions has yet to
be determined. Hence, the present study aims to determine the structural changes and expressions of OPG/RANKL in the
subchondral bone plate and trabecular bone during the development of osteoarthritis in Dunkin Hartley guinea pigs. Eighteen
tibias were scanned using a micro-computed tomography at three different time points; 10, 20 and 30 weeks of age to determine
their subchondral bone plate and trabecular bone thickness. Immunohistochemistry and histopathology methods were conducted
to determine the expression of OPG/RANKL and microscopic osteoarthritis score, respectively. The results showed that
subchondral bone plate and trabecular bone thickness were greater in the medial than the lateral side of the tibial plateau, and
increased significantly with ageing (p < 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp
initially increased, before decreasing at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially
constant but then decreased at 30 weeks of age. Interestingly, a significant correlation was observed between the subchondral
bone plate thickness and OPG/RANKL ratio with microscopic osteoarthritis scores (r = 0.5, p < 0.05), suggesting that the
subchondral bone plate plays an important role in the pathogenesis of osteoarthritis. |
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