High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch

Ionic liquids (ILs) are regarded as green solvents and have demonstrated ability in the solvation of lignocellulosic material, thus promoting its enzymatic hydrolysis by cellulases. In this study, cellulase (PKC-Cel) obtained from Trichoderma reesei (RUTC30) through fermentation displayed good stabi...

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Main Authors:	Elgharbawy, Amal A.M., Alam, Md Zahangir, Moniruzzaman, Muhammad, Kabbashi, Nassereldeen Ahmed, Jamal, Parveen
Format:	Article
Language:	English
Published:	Taylor & Francis 2019
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/70703/ http://irep.iium.edu.my/70703/ http://irep.iium.edu.my/70703/ http://irep.iium.edu.my/70703/1/70703_High%20yield%20of%20second-generation%20ethanol.pdf

id	iium-70703
recordtype	eprints
spelling	iium-707032019-03-06T01:04:42Z http://irep.iium.edu.my/70703/ High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch Elgharbawy, Amal A.M. Alam, Md Zahangir Moniruzzaman, Muhammad Kabbashi, Nassereldeen Ahmed Jamal, Parveen TP248.13 Biotechnology Ionic liquids (ILs) are regarded as green solvents and have demonstrated ability in the solvation of lignocellulosic material, thus promoting its enzymatic hydrolysis by cellulases. In this study, cellulase (PKC-Cel) obtained from Trichoderma reesei (RUTC30) through fermentation displayed good stability in choline acetate [Cho]OAc, a biodegradable IL. Empty fruit bunches (EFB) were subjected to single-step hydrolysis in an IL-enzyme system. Response surface methodology was employed to maximize ethanol production. Three parameters were optimized, namely sugar loading, inoculum concentration, and agitation. The distilled ethanol purity was 98.6% (v/v). The ethanol yield was 87.94% of the theoretical yield, which was 0.275 g ethanol g−1 EFB. In comparison, the non-IL system produced 0.127 g g−1 EFB. The yield coefficients Yps, Yxs, and Ypx were 0.552, 0.148, and 3.69 g g−1. The specific growth rate (μ) was determined to be 0.264 h−1 and the (μmax) was 0.777 h−1. Taylor & Francis 2019-02-13 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70703/1/70703_High%20yield%20of%20second-generation%20ethanol.pdf Elgharbawy, Amal A.M. and Alam, Md Zahangir and Moniruzzaman, Muhammad and Kabbashi, Nassereldeen Ahmed and Jamal, Parveen (2019) High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch. Biofuels, 2019. pp. 1-13. https://www.tandfonline.com/doi/abs/10.1080/17597269.2018.1542569?journalCode=tbfu20 10.1080/17597269.2018.1542569
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Elgharbawy, Amal A.M. Alam, Md Zahangir Moniruzzaman, Muhammad Kabbashi, Nassereldeen Ahmed Jamal, Parveen High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch
description	Ionic liquids (ILs) are regarded as green solvents and have demonstrated ability in the solvation of lignocellulosic material, thus promoting its enzymatic hydrolysis by cellulases. In this study, cellulase (PKC-Cel) obtained from Trichoderma reesei (RUTC30) through fermentation displayed good stability in choline acetate [Cho]OAc, a biodegradable IL. Empty fruit bunches (EFB) were subjected to single-step hydrolysis in an IL-enzyme system. Response surface methodology was employed to maximize ethanol production. Three parameters were optimized, namely sugar loading, inoculum concentration, and agitation. The distilled ethanol purity was 98.6% (v/v). The ethanol yield was 87.94% of the theoretical yield, which was 0.275 g ethanol g−1 EFB. In comparison, the non-IL system produced 0.127 g g−1 EFB. The yield coefficients Yps, Yxs, and Ypx were 0.552, 0.148, and 3.69 g g−1. The specific growth rate (μ) was determined to be 0.264 h−1 and the (μmax) was 0.777 h−1.
format	Article
author	Elgharbawy, Amal A.M. Alam, Md Zahangir Moniruzzaman, Muhammad Kabbashi, Nassereldeen Ahmed Jamal, Parveen
author_facet	Elgharbawy, Amal A.M. Alam, Md Zahangir Moniruzzaman, Muhammad Kabbashi, Nassereldeen Ahmed Jamal, Parveen
author_sort	Elgharbawy, Amal A.M.
title	High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch
title_short	High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch
title_full	High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch
title_fullStr	High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch
title_full_unstemmed	High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch
title_sort	high yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch
publisher	Taylor & Francis
publishDate	2019
url	http://irep.iium.edu.my/70703/ http://irep.iium.edu.my/70703/ http://irep.iium.edu.my/70703/ http://irep.iium.edu.my/70703/1/70703_High%20yield%20of%20second-generation%20ethanol.pdf
first_indexed	2023-09-18T21:40:22Z
last_indexed	2023-09-18T21:40:22Z
_version_	1777413099800756224

High yield of second-generation ethanol in an ionic liquid-cellulase integrated system for single-step processing of empty fruit bunch

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