In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease

In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease

Heart rot disease is one of the major diseases in pineapple cultivation in Malaysia. Josapine and N36 are among varieties that vulnerable to the heart rot disease. The infection can reduce up to 50% yield. In vitro antibacterial activity of four Vernonia amygdalina leaves extract (hexane, chloroform...

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Main Authors: Abdul Aziz, Noorshilawati, Mazlan, Hamimah, Abdullah, Nur Suraya, Omar, Muhammad Nor
Format: Article
Language:English
Published: UPENA Universiti Teknologi MARA Cawangan Pahang 2018
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/70605/
http://irep.iium.edu.my/70605/
http://irep.iium.edu.my/70605/1/Noorshilawati%20Gading%20JST.pdf
id iium-70605
recordtype eprints
spelling iium-706052019-07-15T01:15:52Z http://irep.iium.edu.my/70605/ In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease Abdul Aziz, Noorshilawati Mazlan, Hamimah Abdullah, Nur Suraya Omar, Muhammad Nor QD Chemistry Heart rot disease is one of the major diseases in pineapple cultivation in Malaysia. Josapine and N36 are among varieties that vulnerable to the heart rot disease. The infection can reduce up to 50% yield. In vitro antibacterial activity of four Vernonia amygdalina leaves extract (hexane, chloroform, methanol and aqueous) was carried out and evaluated against pathogen of pineapple heart rot disease. The antibacterial activity was evaluated using well diffusion method where three concentrations for each extract were prepared. The results revealed V. amygdalina chloroform and aqueous leaves extracts at 10000 ppm shows greater inhibition zone than positive control. V. amygdalina hexane leaves extracts, however, did not shows any inhibition zone at any concentrations. Meanwhile, the V. amygdalina methanol leaves extract shows antibacterial activity against the pathogen of pineapple heart rot disease but not as good as positive control. Phytochemical screening was also carried out to determine the presence of alkaloid, flavanoid, glycoside, phenol, saponin, steroid, and tannins as these compounds may attribute to the result. The screening revealed that both V. amygdalina chloroform and aqueous leaves extracts contain alkaloid, saponin, and tannin. Result shows the potentials of V. amygdalina as an antibacterial agent in combating pineapple heart rot disease. UPENA Universiti Teknologi MARA Cawangan Pahang 2018-09-27 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70605/1/Noorshilawati%20Gading%20JST.pdf Abdul Aziz, Noorshilawati and Mazlan, Hamimah and Abdullah, Nur Suraya and Omar, Muhammad Nor (2018) In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease. Gading Journal for Science and Technology, 1 (1). pp. 7-15. E-ISSN 2637-0018 https://gadingst.learningdistance.org/index.php/gadingst/index
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Abdul Aziz, Noorshilawati
Mazlan, Hamimah
Abdullah, Nur Suraya
Omar, Muhammad Nor
In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease
description Heart rot disease is one of the major diseases in pineapple cultivation in Malaysia. Josapine and N36 are among varieties that vulnerable to the heart rot disease. The infection can reduce up to 50% yield. In vitro antibacterial activity of four Vernonia amygdalina leaves extract (hexane, chloroform, methanol and aqueous) was carried out and evaluated against pathogen of pineapple heart rot disease. The antibacterial activity was evaluated using well diffusion method where three concentrations for each extract were prepared. The results revealed V. amygdalina chloroform and aqueous leaves extracts at 10000 ppm shows greater inhibition zone than positive control. V. amygdalina hexane leaves extracts, however, did not shows any inhibition zone at any concentrations. Meanwhile, the V. amygdalina methanol leaves extract shows antibacterial activity against the pathogen of pineapple heart rot disease but not as good as positive control. Phytochemical screening was also carried out to determine the presence of alkaloid, flavanoid, glycoside, phenol, saponin, steroid, and tannins as these compounds may attribute to the result. The screening revealed that both V. amygdalina chloroform and aqueous leaves extracts contain alkaloid, saponin, and tannin. Result shows the potentials of V. amygdalina as an antibacterial agent in combating pineapple heart rot disease.
format Article
author Abdul Aziz, Noorshilawati
Mazlan, Hamimah
Abdullah, Nur Suraya
Omar, Muhammad Nor
author_facet Abdul Aziz, Noorshilawati
Mazlan, Hamimah
Abdullah, Nur Suraya
Omar, Muhammad Nor
author_sort Abdul Aziz, Noorshilawati
title In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease
title_short In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease
title_full In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease
title_fullStr In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease
title_full_unstemmed In vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease
title_sort in vitro antibacterial activity of vernonia amygdalina leaves extracts against pathogen of pineapple heart rot disease
publisher UPENA Universiti Teknologi MARA Cawangan Pahang
publishDate 2018
url http://irep.iium.edu.my/70605/
http://irep.iium.edu.my/70605/
http://irep.iium.edu.my/70605/1/Noorshilawati%20Gading%20JST.pdf
first_indexed 2023-09-18T21:40:13Z
last_indexed 2023-09-18T21:40:13Z
_version_ 1777413090122399744

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