Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production

Oil palm industry (OPI) in Malaysia generates a huge amount of palm oil mill effluent (POME) during the processing of crude palm oil. One ton of fresh fruit brunch produces 0.5 to 0.7 tonnes of POME. The generation of POME by the OPI has been a serious issue to handle due to its high content of CO...

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Main Authors:	Alam, Md. Zahangir, Fazil, Nurul Alia, Azmi, Azlin Suhaida, Mansor, Mariatul Fadzillah
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/70576/ http://irep.iium.edu.my/70576/ http://irep.iium.edu.my/70576/1/70576_Optimization%20of%20biofilm%20mediated%20hydrolytic.pdf

id	iium-70576
recordtype	eprints
spelling	iium-705762019-02-28T01:14:33Z http://irep.iium.edu.my/70576/ Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production Alam, Md. Zahangir Fazil, Nurul Alia Azmi, Azlin Suhaida Mansor, Mariatul Fadzillah TP155 Chemical engineering Oil palm industry (OPI) in Malaysia generates a huge amount of palm oil mill effluent (POME) during the processing of crude palm oil. One ton of fresh fruit brunch produces 0.5 to 0.7 tonnes of POME. The generation of POME by the OPI has been a serious issue to handle due to its high content of COD and BOD. These wastes need proper treatment to be handled as without proper management it can create significant impact towards the environment. Therefore, anaerobic digestion of POME for enhancement of biogas production could be an effective treatment process for sustainable development. In this study, biogas was produced by anaerobic digestion of POME applying biofilm-based technology. Biofilm mediated hydrolysis of POME was carried out to improve hydrolytic enzyme activity towards faster degradation. 2 grams of granular activated carbon (2.8 mg of biofilm per gram carrier) were added as the carrier to the biofilm into the flask experiment containing raw POME and incubated in room temperature with shaking at 150 rpm rotational speed. Several parameters such as hydrolytic activities, free fatty acid (FFA), sugar content were analyzed to evaluate the biofilm performance. A mixture of two to four strains of isolated bacteria had been proven in the earlier study to have better hydrolytic activity with 75.1% improvement (in terms of lipid hydrolysis) compared to control flask (non-sterilized POME with beads addition). Optimization of hydrolytic activity of POME was done using Design-Expert software, Response surface methodology (RSM) based on face-centered central composite design (FCCCD) was used to optimize two important reaction variables – Total suspended solids (TSS) of POME and amount of carrier employed as compared to the hydrolytic compound production. The optimum condition for hydrolysis of POME has been found to be at TSS of 2% with 2 grams of carrier utilized. Using the hydrolysate, the biogas enhancement was observed by 2-3 folds in the treatment process. 2018 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/70576/1/70576_Optimization%20of%20biofilm%20mediated%20hydrolytic.pdf Alam, Md. Zahangir and Fazil, Nurul Alia and Azmi, Azlin Suhaida and Mansor, Mariatul Fadzillah (2018) Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production. In: 4th International Conference on Sustainable Development (ICSD) 2018, 11th-15th April 2018, Athens, Greece. https://www.icsd.eu/sites/default/files/2018_icsd_proceedings_v2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Alam, Md. Zahangir Fazil, Nurul Alia Azmi, Azlin Suhaida Mansor, Mariatul Fadzillah Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production
description	Oil palm industry (OPI) in Malaysia generates a huge amount of palm oil mill effluent (POME) during the processing of crude palm oil. One ton of fresh fruit brunch produces 0.5 to 0.7 tonnes of POME. The generation of POME by the OPI has been a serious issue to handle due to its high content of COD and BOD. These wastes need proper treatment to be handled as without proper management it can create significant impact towards the environment. Therefore, anaerobic digestion of POME for enhancement of biogas production could be an effective treatment process for sustainable development. In this study, biogas was produced by anaerobic digestion of POME applying biofilm-based technology. Biofilm mediated hydrolysis of POME was carried out to improve hydrolytic enzyme activity towards faster degradation. 2 grams of granular activated carbon (2.8 mg of biofilm per gram carrier) were added as the carrier to the biofilm into the flask experiment containing raw POME and incubated in room temperature with shaking at 150 rpm rotational speed. Several parameters such as hydrolytic activities, free fatty acid (FFA), sugar content were analyzed to evaluate the biofilm performance. A mixture of two to four strains of isolated bacteria had been proven in the earlier study to have better hydrolytic activity with 75.1% improvement (in terms of lipid hydrolysis) compared to control flask (non-sterilized POME with beads addition). Optimization of hydrolytic activity of POME was done using Design-Expert software, Response surface methodology (RSM) based on face-centered central composite design (FCCCD) was used to optimize two important reaction variables – Total suspended solids (TSS) of POME and amount of carrier employed as compared to the hydrolytic compound production. The optimum condition for hydrolysis of POME has been found to be at TSS of 2% with 2 grams of carrier utilized. Using the hydrolysate, the biogas enhancement was observed by 2-3 folds in the treatment process.
format	Conference or Workshop Item
author	Alam, Md. Zahangir Fazil, Nurul Alia Azmi, Azlin Suhaida Mansor, Mariatul Fadzillah
author_facet	Alam, Md. Zahangir Fazil, Nurul Alia Azmi, Azlin Suhaida Mansor, Mariatul Fadzillah
author_sort	Alam, Md. Zahangir
title	Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production
title_short	Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production
title_full	Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production
title_fullStr	Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production
title_full_unstemmed	Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production
title_sort	optimization of biofilm mediated hydrolytic activity of pome for enhanced biogas production
publishDate	2018
url	http://irep.iium.edu.my/70576/ http://irep.iium.edu.my/70576/ http://irep.iium.edu.my/70576/1/70576_Optimization%20of%20biofilm%20mediated%20hydrolytic.pdf
first_indexed	2023-09-18T21:40:10Z
last_indexed	2023-09-18T21:40:10Z
_version_	1777413086738644992

Optimization of biofilm mediated hydrolytic activity of POME for enhanced biogas production

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