Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake

Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake

Lipases are important hydrolytic enzymes that catalyze hydrolysis of the triglycerides, esterification and transesterification reactions. These enzymes are of great interest for many reasons include they do not require cofactors, remain active in organic solvent, used with broad range of substrate...

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Main Authors: Zubairi, Hidayah, Alam, Md. Zahangir, Salleh, Md. Noor, Salleh, Hamzah Mohd.
Format: Conference or Workshop Item
Language:English
English
Published: Kulliyyah of Engineering, International Islamic University Malaysia 2018
Subjects:
TP155 Chemical engineering
Online Access:http://irep.iium.edu.my/70575/
http://irep.iium.edu.my/70575/6/70575.pdf
http://irep.iium.edu.my/70575/12/70575_Media%20optimization%20for%20the%20production%20of%20thermostable%20and%20organic%20solvent_scopus.pdf
id iium-70575
recordtype eprints
spelling iium-705752019-07-15T07:05:52Z http://irep.iium.edu.my/70575/ Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake Zubairi, Hidayah Alam, Md. Zahangir Salleh, Md. Noor Salleh, Hamzah Mohd. TP155 Chemical engineering Lipases are important hydrolytic enzymes that catalyze hydrolysis of the triglycerides, esterification and transesterification reactions. These enzymes are of great interest for many reasons include they do not require cofactors, remain active in organic solvent, used with broad range of substrates and exhibit high enatino- and regio- selectivity (Rahman et al., 2005). Very few lipases have been reported having both characteristics such as thermostable and organic solvent-tolerant suitable in fermentation of solid wastes for industrial processes (Masomiama, 2013). Production of thermostable and organic solvent tolerant lipase in solid state fermentation (SSF) using Bacillus smitthi strain PKC-P1 isolated from Palm Kernel Cake (PKC) has been investigated in this study. This strain with 11.13 U/g of lipase activity was optimized by statistical approach. The increment of lipase activity to 46.6 U/g was observed in One-factor-at-a-time (OFAT) studies at 65 o C, pH 10.0, olive oil (1.25%), yeast extract (2.0%), peptone (2.0%) and inoculum size (6%). A successful improvement of lipase production was achieved at the end of this study. This finding revealed and proving that bacterial locally isolated from oil-rich environment has potential as a source of more economical enzyme for biotechnological application. Kulliyyah of Engineering, International Islamic University Malaysia 2018 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/70575/6/70575.pdf application/pdf en http://irep.iium.edu.my/70575/12/70575_Media%20optimization%20for%20the%20production%20of%20thermostable%20and%20organic%20solvent_scopus.pdf Zubairi, Hidayah and Alam, Md. Zahangir and Salleh, Md. Noor and Salleh, Hamzah Mohd. (2018) Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake. In: 5th International Conference on Biotechnology Engineering 2018, 19th-20th September 2018, Kuala Lumpur.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic TP155 Chemical engineering
spellingShingle TP155 Chemical engineering
Zubairi, Hidayah
Alam, Md. Zahangir
Salleh, Md. Noor
Salleh, Hamzah Mohd.
Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake
description Lipases are important hydrolytic enzymes that catalyze hydrolysis of the triglycerides, esterification and transesterification reactions. These enzymes are of great interest for many reasons include they do not require cofactors, remain active in organic solvent, used with broad range of substrates and exhibit high enatino- and regio- selectivity (Rahman et al., 2005). Very few lipases have been reported having both characteristics such as thermostable and organic solvent-tolerant suitable in fermentation of solid wastes for industrial processes (Masomiama, 2013). Production of thermostable and organic solvent tolerant lipase in solid state fermentation (SSF) using Bacillus smitthi strain PKC-P1 isolated from Palm Kernel Cake (PKC) has been investigated in this study. This strain with 11.13 U/g of lipase activity was optimized by statistical approach. The increment of lipase activity to 46.6 U/g was observed in One-factor-at-a-time (OFAT) studies at 65 o C, pH 10.0, olive oil (1.25%), yeast extract (2.0%), peptone (2.0%) and inoculum size (6%). A successful improvement of lipase production was achieved at the end of this study. This finding revealed and proving that bacterial locally isolated from oil-rich environment has potential as a source of more economical enzyme for biotechnological application.
format Conference or Workshop Item
author Zubairi, Hidayah
Alam, Md. Zahangir
Salleh, Md. Noor
Salleh, Hamzah Mohd.
author_facet Zubairi, Hidayah
Alam, Md. Zahangir
Salleh, Md. Noor
Salleh, Hamzah Mohd.
author_sort Zubairi, Hidayah
title Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake
title_short Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake
title_full Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake
title_fullStr Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake
title_full_unstemmed Media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake
title_sort media optimization for the production of thermostable and organic solvent tolerant lipase by solid state fermentation from palm kernel cake
publisher Kulliyyah of Engineering, International Islamic University Malaysia
publishDate 2018
url http://irep.iium.edu.my/70575/
http://irep.iium.edu.my/70575/6/70575.pdf
http://irep.iium.edu.my/70575/12/70575_Media%20optimization%20for%20the%20production%20of%20thermostable%20and%20organic%20solvent_scopus.pdf
first_indexed 2023-09-18T21:40:10Z
last_indexed 2023-09-18T21:40:10Z
_version_ 1777413086597087232

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