Characterization of conformational and oligomeric states of proteins
Oligomerization and conformational changes of proteins in solutions are advantageous for their structural and functional control. The advancement in biophysical characterization analyses, such as Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS) has shed lights in...
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| Language: | English English |
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Springer Nature Singapore
2018
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| Online Access: | http://irep.iium.edu.my/70505/ http://irep.iium.edu.my/70505/ http://irep.iium.edu.my/70505/1/70505_Characterization%20of%20conformational.pdf http://irep.iium.edu.my/70505/7/70505_Characterization%20of%20conformational_Scopus.pdf |
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iium-705052020-03-05T08:42:55Z http://irep.iium.edu.my/70505/ Characterization of conformational and oligomeric states of proteins Ahmad Fuad, Fazia Adyani TP248.13 Biotechnology Oligomerization and conformational changes of proteins in solutions are advantageous for their structural and functional control. The advancement in biophysical characterization analyses, such as Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS) has shed lights in understanding the underlying mechanisms of actions for protein molecules. This chapter focuses on three types of protein analysis; SEC-MALS, native PAGE and continuous enzymatic assays of the bacterially expressed cofactor-independent phosphoglycerate mutase from Leishmania mexicana (Lm-iPGAM), which was shown to exist in different oligomeric and conformational states in solution. The outcome of this analyses has paved the way towards understanding the behavior of Lm-iPGAM in solution, which is important for further structural and functional studies. Springer Nature Singapore Amid, Azura Sulaiman, Sarina Jimat, Dzun Noraini Mohamed Azmin, Nor Fadhillah 2018 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/70505/1/70505_Characterization%20of%20conformational.pdf application/pdf en http://irep.iium.edu.my/70505/7/70505_Characterization%20of%20conformational_Scopus.pdf Ahmad Fuad, Fazia Adyani (2018) Characterization of conformational and oligomeric states of proteins. In: Multifaceted protocol in biotechnology. Springer Nature Singapore, pp. 167-178. ISBN 978-981-13-2256-3 https://www.springer.com/us/book/9789811322563#aboutAuthors |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Ahmad Fuad, Fazia Adyani Characterization of conformational and oligomeric states of proteins |
| description |
Oligomerization and conformational changes of proteins in solutions are
advantageous for their structural and functional control. The advancement in biophysical
characterization analyses, such as Size Exclusion Chromatography coupled
with Multi-Angle Light Scattering (SEC-MALS) has shed lights in
understanding the underlying mechanisms of actions for protein molecules. This
chapter focuses on three types of protein analysis; SEC-MALS, native PAGE and
continuous enzymatic assays of the bacterially expressed cofactor-independent
phosphoglycerate mutase from Leishmania mexicana (Lm-iPGAM), which was
shown to exist in different oligomeric and conformational states in solution. The
outcome of this analyses has paved the way towards understanding the behavior of
Lm-iPGAM in solution, which is important for further structural and functional
studies. |
| author2 |
Amid, Azura |
| author_facet |
Amid, Azura Ahmad Fuad, Fazia Adyani |
| format |
Book Chapter |
| author |
Ahmad Fuad, Fazia Adyani |
| author_sort |
Ahmad Fuad, Fazia Adyani |
| title |
Characterization of conformational and oligomeric states of proteins |
| title_short |
Characterization of conformational and oligomeric states of proteins |
| title_full |
Characterization of conformational and oligomeric states of proteins |
| title_fullStr |
Characterization of conformational and oligomeric states of proteins |
| title_full_unstemmed |
Characterization of conformational and oligomeric states of proteins |
| title_sort |
characterization of conformational and oligomeric states of proteins |
| publisher |
Springer Nature Singapore |
| publishDate |
2018 |
| url |
http://irep.iium.edu.my/70505/ http://irep.iium.edu.my/70505/ http://irep.iium.edu.my/70505/1/70505_Characterization%20of%20conformational.pdf http://irep.iium.edu.my/70505/7/70505_Characterization%20of%20conformational_Scopus.pdf |
| first_indexed |
2023-09-18T21:40:04Z |
| last_indexed |
2023-09-18T21:40:04Z |
| _version_ |
1777413080830967808 |