Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia

Biosurfactants play an important role in bioremediation of organic pollutants such as petroleum hydrocarbon. The unique properties of biosurfactants make them possible to be used in the remediation of hydrocarbon contaminated sites. Therefore, the existence of indigenous microorganisms that have t...

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Main Authors:	Ismail, Muhammad Isminhaziq, Azizan, Nur Hafizah, Mohd Ashaari, Mardiana
Format:	Article
Language:	English
Published:	Zibeline International Publishing Sdn. Bhd. 2018
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/70453/ http://irep.iium.edu.my/70453/ http://irep.iium.edu.my/70453/ http://irep.iium.edu.my/70453/1/Ismin%20et%20al%202018.pdf

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recordtype	eprints
spelling	iium-704532019-07-12T08:31:45Z http://irep.iium.edu.my/70453/ Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia Ismail, Muhammad Isminhaziq Azizan, Nur Hafizah Mohd Ashaari, Mardiana QR Microbiology Biosurfactants play an important role in bioremediation of organic pollutants such as petroleum hydrocarbon. The unique properties of biosurfactants make them possible to be used in the remediation of hydrocarbon contaminated sites. Therefore, the existence of indigenous microorganisms that have the ability to consume petroleum hydrocarbon as carbon source and simultaneously produce biosurfactants in order to facilitate the hydrocarbon metabolism can be manipulated for bioremediation purposes. In this study, isolation and screening of potential biosurfactant-producing bacteria from two sampling points in Kuantan Port seawater were successfully done. Amongst the isolates, 4 out of 7 isolates from Point A were Gram negative bacteria and 2 out 5 isolates from Point B were Gram negative bacteria. The positive oxidase test resulted for all isolates from Point A and only B5 from Point B produced negative result. Catalase test conducted produced positive results on isolates from Point A (A3, A5, A6& A7) and Point B (B1, B2, B4 & B5).The highest percentage emulsification index measured belonged to isolate B4 and B5 which are 67%, thus make these isolates to be the most promising biosurfactant producers. Further identification by 16S rRNA gene found that isolates were closely related to Rhodococcus erythropolis (A1), Psedomonas stutzeri (A2), Pseudoalteromonas lipolytica (A3, A6 and B4), Vibrio brasiliensis (A4 and B2), Vibrio tubiashii (B1), Marinobacter salsuginis (A5), Labrenzia aggregate (A7), Marinococcus halophilus (B3) and Thalassospira xianmenensis(B5). Hence, through biosurfactant activities exhibited by isolates, B4 and B5 were the most potential isolates to produce biosurfactant. Therefore, these isolates can potentially be exploited to aid in bioremediation of petroleum hydrocarbon contaminated sites and would also be useful to enhance oil recovery in petroleum industry. Zibeline International Publishing Sdn. Bhd. 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70453/1/Ismin%20et%20al%202018.pdf Ismail, Muhammad Isminhaziq and Azizan, Nur Hafizah and Mohd Ashaari, Mardiana (2018) Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia. Science Heritage Journal, 2 (2). pp. 21-26. ISSN 2521-0858 E-ISSN 2521-0866 https://zibelinepub.com/archives/2gws2018/2gws2018-21-26.pdf 10.26480/gws.02.2018.21.26
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Ismail, Muhammad Isminhaziq Azizan, Nur Hafizah Mohd Ashaari, Mardiana Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia
description	Biosurfactants play an important role in bioremediation of organic pollutants such as petroleum hydrocarbon. The unique properties of biosurfactants make them possible to be used in the remediation of hydrocarbon contaminated sites. Therefore, the existence of indigenous microorganisms that have the ability to consume petroleum hydrocarbon as carbon source and simultaneously produce biosurfactants in order to facilitate the hydrocarbon metabolism can be manipulated for bioremediation purposes. In this study, isolation and screening of potential biosurfactant-producing bacteria from two sampling points in Kuantan Port seawater were successfully done. Amongst the isolates, 4 out of 7 isolates from Point A were Gram negative bacteria and 2 out 5 isolates from Point B were Gram negative bacteria. The positive oxidase test resulted for all isolates from Point A and only B5 from Point B produced negative result. Catalase test conducted produced positive results on isolates from Point A (A3, A5, A6& A7) and Point B (B1, B2, B4 & B5).The highest percentage emulsification index measured belonged to isolate B4 and B5 which are 67%, thus make these isolates to be the most promising biosurfactant producers. Further identification by 16S rRNA gene found that isolates were closely related to Rhodococcus erythropolis (A1), Psedomonas stutzeri (A2), Pseudoalteromonas lipolytica (A3, A6 and B4), Vibrio brasiliensis (A4 and B2), Vibrio tubiashii (B1), Marinobacter salsuginis (A5), Labrenzia aggregate (A7), Marinococcus halophilus (B3) and Thalassospira xianmenensis(B5). Hence, through biosurfactant activities exhibited by isolates, B4 and B5 were the most potential isolates to produce biosurfactant. Therefore, these isolates can potentially be exploited to aid in bioremediation of petroleum hydrocarbon contaminated sites and would also be useful to enhance oil recovery in petroleum industry.
format	Article
author	Ismail, Muhammad Isminhaziq Azizan, Nur Hafizah Mohd Ashaari, Mardiana
author_facet	Ismail, Muhammad Isminhaziq Azizan, Nur Hafizah Mohd Ashaari, Mardiana
author_sort	Ismail, Muhammad Isminhaziq
title	Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia
title_short	Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia
title_full	Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia
title_fullStr	Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia
title_full_unstemmed	Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia
title_sort	isolation and screening of biosurfactant-producing marine bacteria from kuantan port, pahang, malaysia
publisher	Zibeline International Publishing Sdn. Bhd.
publishDate	2018
url	http://irep.iium.edu.my/70453/ http://irep.iium.edu.my/70453/ http://irep.iium.edu.my/70453/ http://irep.iium.edu.my/70453/1/Ismin%20et%20al%202018.pdf
first_indexed	2023-09-18T21:40:01Z
last_indexed	2023-09-18T21:40:01Z
_version_	1777413077402124288

Isolation and screening of biosurfactant-producing marine bacteria from Kuantan Port, Pahang, Malaysia

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