Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production

Aims: Biofilm is a complex structure that provides protection towards the bacteria within the barrier. Enhanced biogas production from Palm Oil Mill Effluent (POME) can be achieved by applying biofilm based anaerobic digestion system. Methodology and results: Bacteria that produces biofilm were is...

Full description

Bibliographic Details
Main Authors:	Fazdil, Nurul Alia, Alam, Md. Zahangir, Azmi, Azlin Suhaida, Mansor, Mariatul Fadzillah, Mohd Zubairi, Nurul Hidayah
Format:	Article
Language:	English English English
Published:	Malaysian Society for Microbiology 2018
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/70435/ http://irep.iium.edu.my/70435/ http://irep.iium.edu.my/70435/1/Paper%20MJM%20Alia.pdf http://irep.iium.edu.my/70435/7/70435_Isolation%20and%20screening%20of%20bacteria%20with%20biofilm_scopus.pdf http://irep.iium.edu.my/70435/8/70435_Isolation%20and%20screening%20of%20bacteria%20with%20biofilm_wos.pdf

id	iium-70435
recordtype	eprints
spelling	iium-704352019-07-12T08:32:19Z http://irep.iium.edu.my/70435/ Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production Fazdil, Nurul Alia Alam, Md. Zahangir Azmi, Azlin Suhaida Mansor, Mariatul Fadzillah Mohd Zubairi, Nurul Hidayah TP248.13 Biotechnology Aims: Biofilm is a complex structure that provides protection towards the bacteria within the barrier. Enhanced biogas production from Palm Oil Mill Effluent (POME) can be achieved by applying biofilm based anaerobic digestion system. Methodology and results: Bacteria that produces biofilm were isolated and tested on its hydrolytic enzyme secretion. The biofilm produced were also characterized. Out of 120 strains isolated from POME, PKC and food waste compost, only 33 strains were producing biofilm and only 11 of them exhibited significant amount of biofilm produced at optical density of wavelength 595 nm (>0.01). In hydrolysis enzyme assay test, all strains were not able to secrete protease enzyme. The biofilms were extracted and characterized to show similar characteristic for all strains. Strain numbers of 11, 9C, 23C and 30C showed positive result for cellulase, amylase and lipase enzymes, to be tested as single strain bacteria and also mixed with other isolated bacterium for prospect research on effective hydrolysis towards enhanced biogas production. The composition of biofilms from different bacteria mixture also similar under the same incubation condition. Conclusion, significance and impact of study: Bacteria producing biofilm are very limited and does not secrete the same hydrolytic enzymes. Utilization of these bacteria may eliminate the problem of microbial instability in a system. Malaysian Society for Microbiology 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70435/1/Paper%20MJM%20Alia.pdf application/pdf en http://irep.iium.edu.my/70435/7/70435_Isolation%20and%20screening%20of%20bacteria%20with%20biofilm_scopus.pdf application/pdf en http://irep.iium.edu.my/70435/8/70435_Isolation%20and%20screening%20of%20bacteria%20with%20biofilm_wos.pdf Fazdil, Nurul Alia and Alam, Md. Zahangir and Azmi, Azlin Suhaida and Mansor, Mariatul Fadzillah and Mohd Zubairi, Nurul Hidayah (2018) Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production. Malaysian Journal of Microbiology, 14 (2 ( Special Issue )). pp. 96-101. ISSN 1823-8262 E-ISSN 2231-7538 http://mjm.usm.my/uploads/issues/1386/MJM%202018-3-90.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Fazdil, Nurul Alia Alam, Md. Zahangir Azmi, Azlin Suhaida Mansor, Mariatul Fadzillah Mohd Zubairi, Nurul Hidayah Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production
description	Aims: Biofilm is a complex structure that provides protection towards the bacteria within the barrier. Enhanced biogas production from Palm Oil Mill Effluent (POME) can be achieved by applying biofilm based anaerobic digestion system. Methodology and results: Bacteria that produces biofilm were isolated and tested on its hydrolytic enzyme secretion. The biofilm produced were also characterized. Out of 120 strains isolated from POME, PKC and food waste compost, only 33 strains were producing biofilm and only 11 of them exhibited significant amount of biofilm produced at optical density of wavelength 595 nm (>0.01). In hydrolysis enzyme assay test, all strains were not able to secrete protease enzyme. The biofilms were extracted and characterized to show similar characteristic for all strains. Strain numbers of 11, 9C, 23C and 30C showed positive result for cellulase, amylase and lipase enzymes, to be tested as single strain bacteria and also mixed with other isolated bacterium for prospect research on effective hydrolysis towards enhanced biogas production. The composition of biofilms from different bacteria mixture also similar under the same incubation condition. Conclusion, significance and impact of study: Bacteria producing biofilm are very limited and does not secrete the same hydrolytic enzymes. Utilization of these bacteria may eliminate the problem of microbial instability in a system.
format	Article
author	Fazdil, Nurul Alia Alam, Md. Zahangir Azmi, Azlin Suhaida Mansor, Mariatul Fadzillah Mohd Zubairi, Nurul Hidayah
author_facet	Fazdil, Nurul Alia Alam, Md. Zahangir Azmi, Azlin Suhaida Mansor, Mariatul Fadzillah Mohd Zubairi, Nurul Hidayah
author_sort	Fazdil, Nurul Alia
title	Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production
title_short	Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production
title_full	Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production
title_fullStr	Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production
title_full_unstemmed	Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production
title_sort	isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production
publisher	Malaysian Society for Microbiology
publishDate	2018
url	http://irep.iium.edu.my/70435/ http://irep.iium.edu.my/70435/ http://irep.iium.edu.my/70435/1/Paper%20MJM%20Alia.pdf http://irep.iium.edu.my/70435/7/70435_Isolation%20and%20screening%20of%20bacteria%20with%20biofilm_scopus.pdf http://irep.iium.edu.my/70435/8/70435_Isolation%20and%20screening%20of%20bacteria%20with%20biofilm_wos.pdf
first_indexed	2023-09-18T21:40:00Z
last_indexed	2023-09-18T21:40:00Z
_version_	1777413075954040832

Isolation and screening of bacteria with biofilm formation ability and characterization with hydrolytic enzyme production for enhanced biogas production

Similar Items