KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area

Wound closure skin contraction occurs simultaneously as part of the repairing process to restore barrier function and protection after an injury. The process of repair without the use of optimized regenerative template from collagen type I, is often accompanied by the formation of scar and skin cont...

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Main Authors:	Shahbuddin, Munira, Bullock, Anthony J., Rimmer, Stephen, MacNeil, Sheila
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	Q Science (General) TA166 Human engineering
Online Access:	http://irep.iium.edu.my/70361/ http://irep.iium.edu.my/70361/1/APASTB2018%20182-128%20MUNIRA%20SHAHBUDDIN.pdf http://irep.iium.edu.my/70361/9/45868.pdf

id	iium-70361
recordtype	eprints
spelling	iium-703612019-03-08T01:44:25Z http://irep.iium.edu.my/70361/ KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area Shahbuddin, Munira Bullock, Anthony J. Rimmer, Stephen MacNeil, Sheila Q Science (General) TA166 Human engineering Wound closure skin contraction occurs simultaneously as part of the repairing process to restore barrier function and protection after an injury. The process of repair without the use of optimized regenerative template from collagen type I, is often accompanied by the formation of scar and skin contraction that cause disfigurement and impaired movements. The work by Yannas et al. elucidated antagonistic relation between wound closure by contraction and regeneration that scar formation is a process secondary to wound contraction which can be prevented simply by early intervention of contraction blocking. A previous study in MacNeil group showed that the contraction of tissue engineered skin based on human dermis appeared to be related to the differentiation status of the keratinocytes. Although Yannas’ work on the cancellation of contraction leading to scarless wound healing was conducted in a well-defined animal model, it was interesting to see the effects of plant heteropolysaccharide, konjac glucomannan (KGM), which is known for its selective effects on the inhibition of keratinocyte proliferation and migration which lead to the inhibition of the contraction of 3D tissue engineered (TE) skin model. This study was aimed to examine the relation between physicochemistry of KGM in soluble and insoluble form; namely interpenetrating network and graft co-network hydrogel on cell viability and phenotype as well as skin contraction and appearance. The biological activities of soluble and insoluble KGM suggest two mechanisms of action in self reorganization and reepithelization of 3D TE skin model: 1) the stimulation of fibroblast proliferation in the dermal area encourages keratinocyte migration for re-epithelisation and 2) the inhibition of keratinocyte proliferation helped to reduce skin contraction that would be beneficial to wound healing. 2018-08-27 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/70361/1/APASTB2018%20182-128%20MUNIRA%20SHAHBUDDIN.pdf application/pdf en http://irep.iium.edu.my/70361/9/45868.pdf Shahbuddin, Munira and Bullock, Anthony J. and Rimmer, Stephen and MacNeil, Sheila (2018) KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area. In: 17th International Conference of Asia Pacific Association of Surgical Tissue Banks (APASTB) 2018, 27th-30th 2018, Putrajaya. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General) TA166 Human engineering
spellingShingle	Q Science (General) TA166 Human engineering Shahbuddin, Munira Bullock, Anthony J. Rimmer, Stephen MacNeil, Sheila KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area
description	Wound closure skin contraction occurs simultaneously as part of the repairing process to restore barrier function and protection after an injury. The process of repair without the use of optimized regenerative template from collagen type I, is often accompanied by the formation of scar and skin contraction that cause disfigurement and impaired movements. The work by Yannas et al. elucidated antagonistic relation between wound closure by contraction and regeneration that scar formation is a process secondary to wound contraction which can be prevented simply by early intervention of contraction blocking. A previous study in MacNeil group showed that the contraction of tissue engineered skin based on human dermis appeared to be related to the differentiation status of the keratinocytes. Although Yannas’ work on the cancellation of contraction leading to scarless wound healing was conducted in a well-defined animal model, it was interesting to see the effects of plant heteropolysaccharide, konjac glucomannan (KGM), which is known for its selective effects on the inhibition of keratinocyte proliferation and migration which lead to the inhibition of the contraction of 3D tissue engineered (TE) skin model. This study was aimed to examine the relation between physicochemistry of KGM in soluble and insoluble form; namely interpenetrating network and graft co-network hydrogel on cell viability and phenotype as well as skin contraction and appearance. The biological activities of soluble and insoluble KGM suggest two mechanisms of action in self reorganization and reepithelization of 3D TE skin model: 1) the stimulation of fibroblast proliferation in the dermal area encourages keratinocyte migration for re-epithelisation and 2) the inhibition of keratinocyte proliferation helped to reduce skin contraction that would be beneficial to wound healing.
format	Conference or Workshop Item
author	Shahbuddin, Munira Bullock, Anthony J. Rimmer, Stephen MacNeil, Sheila
author_facet	Shahbuddin, Munira Bullock, Anthony J. Rimmer, Stephen MacNeil, Sheila
author_sort	Shahbuddin, Munira
title	KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area
title_short	KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area
title_full	KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area
title_fullStr	KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area
title_full_unstemmed	KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area
title_sort	kgm hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area
publishDate	2018
url	http://irep.iium.edu.my/70361/ http://irep.iium.edu.my/70361/1/APASTB2018%20182-128%20MUNIRA%20SHAHBUDDIN.pdf http://irep.iium.edu.my/70361/9/45868.pdf
first_indexed	2023-09-18T21:39:54Z
last_indexed	2023-09-18T21:39:54Z
_version_	1777413069612253184

KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area

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