Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100...

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Main Authors:	Karim, Ahasanul, Yousuf, Abu, Islam, Mohammed Amirul, Hashim, Yasir, Faizal, Che Ku Mohammad, Alam, Md Zahangir, Pirozzi, Domenico
Format:	Article
Language:	English English
Published:	Wiley-Blackwell 2018
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/25/70317_Microbial%20Lipid%20Extraction%20from%20Lipomyces%20starkeyi_article.pdf http://irep.iium.edu.my/70317/14/70317%20Microbial%20lipid%20extraction%20from%20Lipomyces%20starkeyi%20SCOPUS.pdf

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spelling	iium-703172019-09-04T08:58:15Z http://irep.iium.edu.my/70317/ Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation Karim, Ahasanul Yousuf, Abu Islam, Mohammed Amirul Hashim, Yasir Faizal, Che Ku Mohammad Alam, Md Zahangir Pirozzi, Domenico TP Chemical technology TP248.13 Biotechnology The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m ). At this condition, maximum lipid (63 mg g ) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass. © 2018 American Institute of Chemical Engineers Biotechnol. Wiley-Blackwell 2018-08 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/70317/25/70317_Microbial%20Lipid%20Extraction%20from%20Lipomyces%20starkeyi_article.pdf application/pdf en http://irep.iium.edu.my/70317/14/70317%20Microbial%20lipid%20extraction%20from%20Lipomyces%20starkeyi%20SCOPUS.pdf Karim, Ahasanul and Yousuf, Abu and Islam, Mohammed Amirul and Hashim, Yasir and Faizal, Che Ku Mohammad and Alam, Md Zahangir and Pirozzi, Domenico (2018) Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation. Biotechnology Progress, 34 (4). pp. 838-845. ISSN 8756-7938 E-ISSN 1520-6033 https://onlinelibrary.wiley.com/doi/10.1002/btpr.2625 10.1002/btpr.2625
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Karim, Ahasanul Yousuf, Abu Islam, Mohammed Amirul Hashim, Yasir Faizal, Che Ku Mohammad Alam, Md Zahangir Pirozzi, Domenico Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
description	The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m ). At this condition, maximum lipid (63 mg g ) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass. © 2018 American Institute of Chemical Engineers Biotechnol.
format	Article
author	Karim, Ahasanul Yousuf, Abu Islam, Mohammed Amirul Hashim, Yasir Faizal, Che Ku Mohammad Alam, Md Zahangir Pirozzi, Domenico
author_facet	Karim, Ahasanul Yousuf, Abu Islam, Mohammed Amirul Hashim, Yasir Faizal, Che Ku Mohammad Alam, Md Zahangir Pirozzi, Domenico
author_sort	Karim, Ahasanul
title	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_short	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_full	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_fullStr	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_full_unstemmed	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_sort	microbial lipid extraction from lipomyces starkeyi using irreversible electroporation
publisher	Wiley-Blackwell
publishDate	2018
url	http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/25/70317_Microbial%20Lipid%20Extraction%20from%20Lipomyces%20starkeyi_article.pdf http://irep.iium.edu.my/70317/14/70317%20Microbial%20lipid%20extraction%20from%20Lipomyces%20starkeyi%20SCOPUS.pdf
first_indexed	2023-09-18T21:39:50Z
last_indexed	2023-09-18T21:39:50Z
_version_	1777413065540632576

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

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