Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Wiley-Blackwell
2018
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/ http://irep.iium.edu.my/70317/25/70317_Microbial%20Lipid%20Extraction%20from%20Lipomyces%20starkeyi_article.pdf http://irep.iium.edu.my/70317/14/70317%20Microbial%20lipid%20extraction%20from%20Lipomyces%20starkeyi%20SCOPUS.pdf |
| Summary: | The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a
microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly
manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of
square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min
where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units
(CFU) were counted to observe the cell viability under the high voltage electric field. The forces of
the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of
microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After
breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other
techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the
distance of 2 cm, which provided high treatment intensity (36.7 kWh m ). At this condition,
maximum lipid (63 mg g ) was extracted when the biomass was treated for 10 min. During the
comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8%
for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the
time and solvent usage for lipid extraction from microbial biomass. © 2018 American Institute of
Chemical Engineers Biotechnol. |
|---|