Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)

Introduction:Christia vespertilionisis is a plant herb that has been used traditionally due to its benefits for potentially to treat some disease. It contain potential phytochemical compound that can be a component of drugs or natural products. This study was conducted to investigate the total phen...

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Main Authors:	Mat Jusoh, Hanapi, Zulkarnail, Mohamad Zharif, Haron, Normah
Format:	Article
Language:	English
Published:	IIUM Press 2019
Subjects:	Q Science (General) QD Chemistry
Online Access:	http://irep.iium.edu.my/70263/ http://irep.iium.edu.my/70263/ http://irep.iium.edu.my/70263/1/180-Article%20Text-1128-1-10-20190805%20daun%20rerama.pdf

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spelling	iium-702632019-09-11T07:23:39Z http://irep.iium.edu.my/70263/ Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama) Mat Jusoh, Hanapi Zulkarnail, Mohamad Zharif Haron, Normah Q Science (General) QD Chemistry Introduction:Christia vespertilionisis is a plant herb that has been used traditionally due to its benefits for potentially to treat some disease. It contain potential phytochemical compound that can be a component of drugs or natural products. This study was conducted to investigate the total phenolic content and its antioxidant radical scavenging activity on C. vespertilionisis. Hence, a series of test in determining the total phenolic content in three different type of extractions was conducted and its relationship with antioxidant scavenging activity was studied. The C. vespertilionis’s powder was extracted with 80% methanol and hydrolyzed with HCI and NaOH. The total phenolic content was determined using Follin-Ciocalteu and DPPH assay was used for antioxidant activity. Based on the test, the total phenolic content of all extracts was 9.13± 0.13 mg of GAE/g with insoluble bound extracts revealed a highest value of 4.94 ± 0.042 mg of GAE/g followed by soluble bound and free phenolic extracts with (3.16± 0.28 and 0.98 ± 0.07 mg of GAE/g respectively), (p<0.05). The antioxidant scavenging capacity extracts of IC50 showed that insoluble bound extracts showed lowest value (38.13 ± 0.65mg/ml) followed by soluble bound extracts and free phenolic extracts with (39.54 ± 0.77 and 42.59 ± 1.69mg/ml respectively). IIUM Press 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70263/1/180-Article%20Text-1128-1-10-20190805%20daun%20rerama.pdf Mat Jusoh, Hanapi and Zulkarnail, Mohamad Zharif and Haron, Normah (2019) Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama). International Journal of Allied Health Sciences, 3 (2). pp. 649-657. E-ISSN 2600-8491 https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/180
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QD Chemistry
spellingShingle	Q Science (General) QD Chemistry Mat Jusoh, Hanapi Zulkarnail, Mohamad Zharif Haron, Normah Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)
description	Introduction:Christia vespertilionisis is a plant herb that has been used traditionally due to its benefits for potentially to treat some disease. It contain potential phytochemical compound that can be a component of drugs or natural products. This study was conducted to investigate the total phenolic content and its antioxidant radical scavenging activity on C. vespertilionisis. Hence, a series of test in determining the total phenolic content in three different type of extractions was conducted and its relationship with antioxidant scavenging activity was studied. The C. vespertilionis’s powder was extracted with 80% methanol and hydrolyzed with HCI and NaOH. The total phenolic content was determined using Follin-Ciocalteu and DPPH assay was used for antioxidant activity. Based on the test, the total phenolic content of all extracts was 9.13± 0.13 mg of GAE/g with insoluble bound extracts revealed a highest value of 4.94 ± 0.042 mg of GAE/g followed by soluble bound and free phenolic extracts with (3.16± 0.28 and 0.98 ± 0.07 mg of GAE/g respectively), (p<0.05). The antioxidant scavenging capacity extracts of IC50 showed that insoluble bound extracts showed lowest value (38.13 ± 0.65mg/ml) followed by soluble bound extracts and free phenolic extracts with (39.54 ± 0.77 and 42.59 ± 1.69mg/ml respectively).
format	Article
author	Mat Jusoh, Hanapi Zulkarnail, Mohamad Zharif Haron, Normah
author_facet	Mat Jusoh, Hanapi Zulkarnail, Mohamad Zharif Haron, Normah
author_sort	Mat Jusoh, Hanapi
title	Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)
title_short	Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)
title_full	Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)
title_fullStr	Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)
title_full_unstemmed	Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)
title_sort	total phenolic contents and antioxidant scavenging capacity in free and bound extracts of christia vespertilionisis (daun rerama)
publisher	IIUM Press
publishDate	2019
url	http://irep.iium.edu.my/70263/ http://irep.iium.edu.my/70263/ http://irep.iium.edu.my/70263/1/180-Article%20Text-1128-1-10-20190805%20daun%20rerama.pdf
first_indexed	2023-09-18T21:39:45Z
last_indexed	2023-09-18T21:39:45Z
_version_	1777413060532633600

Total phenolic contents and antioxidant scavenging capacity in free and bound extracts of Christia vespertilionisis (daun rerama)

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