Construction of metagenomic DNA libraries and enrichment strategies

Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible to overcome the major obstacle of microbiology which lies on the inability of the vast majority of microbes to grow under laboratory conditions. This chapter introduces and guides readers to a molecu...

Full description

Bibliographic Details
Main Authors:	Benbelgacem, Farah Fadwa, Mohd. Salleh, Hamzah, Noorbatcha, Ibrahim Ali
Other Authors:	Amid, Azura
Format:	Book Chapter
Language:	English English
Published:	Springer Nature 2018
Subjects:	TP200 Manufacture and use of chemicals TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/70247/ http://irep.iium.edu.my/70247/ http://irep.iium.edu.my/70247/1/70247_Construction%20of%20metagenomic%20DNA%20libraries.pdf http://irep.iium.edu.my/70247/7/70247_Construction%20of%20metagenomic%20DNA%20libraries_SCOPUS.pdf

id	iium-70247
recordtype	eprints
spelling	iium-702472020-03-05T01:35:51Z http://irep.iium.edu.my/70247/ Construction of metagenomic DNA libraries and enrichment strategies Benbelgacem, Farah Fadwa Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali TP200 Manufacture and use of chemicals TP248.13 Biotechnology Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible to overcome the major obstacle of microbiology which lies on the inability of the vast majority of microbes to grow under laboratory conditions. This chapter introduces and guides readers to a molecular biology approach that is useful for bioprospecting of novel, efficient and specific enzymes without escaping any unculturable or difficult to culture microorgaims. The first and necessary step of functional metagenomics is the metagenomic DNA library construction. It consists of metagenome extraction from the desired habitat, shearing of the DNA into sequences of desired size, endrepairing and cloning of these DNA sequences into a large-insert and high-copy number vector such as pCC1FOS with EPI300T1R E. coli as surrogate host, to the final step of storing the clones in glycerol stock for long term storage. Enrichment pre-DNA extraction step may be adopted to increase the likelihood that metagenomic DNA libraries hold the genes for the desired enzymatic activities. Springer Nature Amid, Azura Sulaiman, Sarina Jimat, Dzun Noraini Mohamed Azmin, Nor Fadhillah 2018 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/70247/1/70247_Construction%20of%20metagenomic%20DNA%20libraries.pdf application/pdf en http://irep.iium.edu.my/70247/7/70247_Construction%20of%20metagenomic%20DNA%20libraries_SCOPUS.pdf Benbelgacem, Farah Fadwa and Mohd. Salleh, Hamzah and Noorbatcha, Ibrahim Ali (2018) Construction of metagenomic DNA libraries and enrichment strategies. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 23-42. ISBN 978-981-13-2256-3 https://www.springer.com/us/book/9789811322563
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP200 Manufacture and use of chemicals TP248.13 Biotechnology
spellingShingle	TP200 Manufacture and use of chemicals TP248.13 Biotechnology Benbelgacem, Farah Fadwa Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali Construction of metagenomic DNA libraries and enrichment strategies
description	Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible to overcome the major obstacle of microbiology which lies on the inability of the vast majority of microbes to grow under laboratory conditions. This chapter introduces and guides readers to a molecular biology approach that is useful for bioprospecting of novel, efficient and specific enzymes without escaping any unculturable or difficult to culture microorgaims. The first and necessary step of functional metagenomics is the metagenomic DNA library construction. It consists of metagenome extraction from the desired habitat, shearing of the DNA into sequences of desired size, endrepairing and cloning of these DNA sequences into a large-insert and high-copy number vector such as pCC1FOS with EPI300T1R E. coli as surrogate host, to the final step of storing the clones in glycerol stock for long term storage. Enrichment pre-DNA extraction step may be adopted to increase the likelihood that metagenomic DNA libraries hold the genes for the desired enzymatic activities.
author2	Amid, Azura
author_facet	Amid, Azura Benbelgacem, Farah Fadwa Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali
format	Book Chapter
author	Benbelgacem, Farah Fadwa Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali
author_sort	Benbelgacem, Farah Fadwa
title	Construction of metagenomic DNA libraries and enrichment strategies
title_short	Construction of metagenomic DNA libraries and enrichment strategies
title_full	Construction of metagenomic DNA libraries and enrichment strategies
title_fullStr	Construction of metagenomic DNA libraries and enrichment strategies
title_full_unstemmed	Construction of metagenomic DNA libraries and enrichment strategies
title_sort	construction of metagenomic dna libraries and enrichment strategies
publisher	Springer Nature
publishDate	2018
url	http://irep.iium.edu.my/70247/ http://irep.iium.edu.my/70247/ http://irep.iium.edu.my/70247/1/70247_Construction%20of%20metagenomic%20DNA%20libraries.pdf http://irep.iium.edu.my/70247/7/70247_Construction%20of%20metagenomic%20DNA%20libraries_SCOPUS.pdf
first_indexed	2023-09-18T21:39:43Z
last_indexed	2023-09-18T21:39:43Z
_version_	1777413058664071168

Construction of metagenomic DNA libraries and enrichment strategies

Similar Items