Site-directed mutagenesis on plasmid using polymerase chain reaction
Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid ha...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Book Chapter |
| Language: | English English |
| Published: |
Springer Nature
2018
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf |