GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity
ABSTRACT Different extraction processes were employed to extract bioactive metabolites from Salacca zalacca flesh by a range of aqueous and organic solvents. The highest extraction yield was obtained by 50% ethanol extract of SE (73.18 ±4.35%), whereas SFE_1 showed the lowest yield (0.42 ±0.08%). A...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor & Francis
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/1/Natural%20Product%20Research%20_2019%20Salak.pdf http://irep.iium.edu.my/70206/7/70206_GC-MS%20analysis%20of%20metabolites%20from%20soxhlet%20extraction%2C%20ultrasound-assisted%20extraction%20and%20supercritical%20fluid%20extraction%20of%20Salacca_scopus.pdf |
| Summary: | ABSTRACT
Different extraction processes were employed to extract bioactive metabolites from Salacca zalacca flesh by a range of aqueous and organic solvents. The highest extraction yield was obtained by 50% ethanol extract of SE (73.18 ±4.35%), whereas SFE_1 showed the lowest yield (0.42 ±0.08%). All extracts were evaluated for in vitro a-glucosidase inhibitory activity, measured by their IC50 values in comparison to that of quercetin, the positive control (IC50 ¼ 2.7 ±0.7 lg/mL). The lowest a-glucosidase inhibitory activity was
indicated by water extract of SE (IC50 ¼ 724.3 ±42.9 lg/mL) and
the highest activity was demonstrated by 60% ethanol extract by
UAE (IC50 ¼ 16.2 ± 2.4 lg/mL). All extracts were analysed by GC-MS and identified metabolites like carbohydrates, fatty acids, organic acids, phenolic acids, sterols and alkane-based compounds etcetera that may possess the potential as a-glucosidase inhibitor and may attribute to the a-glucosidase inhibitory activity. |
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