Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation
Objective: The aim of this study is to investigate the antihyperglycemic activity of tinocrisposide by stimulating 3T3-L1 adipocyte cell differentiation. Tinocrisposide is a furanoditerpene glycoside that was isolated from Tinospora crispa (Menispermaceae). Methods: Adipocyte cell differentiation...
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Asian Journal of Pharmaceutical and Clinical Research
2018
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iium-700382019-04-09T03:39:53Z http://irep.iium.edu.my/70038/ Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation Adnan, Adek Zamrud Bakhtiar, Muhammad Taher ,, Annisa Fauzana Afriani, Tika Roesma, Dewi Imelda Putra, Andani Eka RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology Objective: The aim of this study is to investigate the antihyperglycemic activity of tinocrisposide by stimulating 3T3-L1 adipocyte cell differentiation. Tinocrisposide is a furanoditerpene glycoside that was isolated from Tinospora crispa (Menispermaceae). Methods: Adipocyte cell differentiation activity of tinocrisposide in interval concentrations of 50, 25, 12.5, and 6.25 µg/ml has been investigated on 3T3-L1 cell line using insulin of 1 µg/ml as a positive and Dulbecco’s modified Eagle media (DMEM) as a negative control group. The effect of tinocrisposide was quantified with oil red O staining method by measuring an absorbance of lipid solution in isopropanol at a wavelength (λ) of 520 nm. Results: Tinocrisposide in the concentrations of 50, 25, 12.5, and 6.25 µg/ml insulin of 1 µg/ml and DMEM groups showed absorbance value of 0.7669, 0.7253, 0.6563, 0.6481, 0.954, and 0.2653, respectively. It was found that there was a significant difference statistically in lipid droplets accumulation among all groups (p<0.05) and tinocrisposide at a concentration of 50 µg/ml showed the highest lipid droplets accumulation in 3T3-L1 adipocyte cells. Conclusion: From the study, it could be concluded that tinocrisposide was able to stimulate the differentiation of adipocyte cell and had antihyperglycemic activity Asian Journal of Pharmaceutical and Clinical Research 2018 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/70038/1/70038_Antihyperglycemic%20activity%20of%20tinocrisposide%20by%20stimulating_article.pdf application/pdf en http://irep.iium.edu.my/70038/2/70038_Antihyperglycemic%20activity%20of%20tinocrisposide%20by%20stimulating_scopus.pdf Adnan, Adek Zamrud and Bakhtiar, Muhammad Taher and ,, Annisa Fauzana and Afriani, Tika and Roesma, Dewi Imelda and Putra, Andani Eka (2018) Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation. Asian Journal of Pharmaceutical and Clinical Research, 11 (11). pp. 494-498. ISSN 0974-2441 E-ISSN 2455-3891 https://innovareacademics.in/journals/index.php/ajpcr/index 10.22159/ajpcr.2018.v11i11.29304 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology |
| spellingShingle |
RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology Adnan, Adek Zamrud Bakhtiar, Muhammad Taher ,, Annisa Fauzana Afriani, Tika Roesma, Dewi Imelda Putra, Andani Eka Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation |
| description |
Objective: The aim of this study is to investigate the antihyperglycemic activity of tinocrisposide by stimulating 3T3-L1 adipocyte cell differentiation.
Tinocrisposide is a furanoditerpene glycoside that was isolated from Tinospora crispa (Menispermaceae).
Methods: Adipocyte cell differentiation activity of tinocrisposide in interval concentrations of 50, 25, 12.5, and 6.25 µg/ml has been investigated
on 3T3-L1 cell line using insulin of 1 µg/ml as a positive and Dulbecco’s modified Eagle media (DMEM) as a negative control group. The effect of
tinocrisposide was quantified with oil red O staining method by measuring an absorbance of lipid solution in isopropanol at a wavelength (λ) of
520 nm.
Results: Tinocrisposide in the concentrations of 50, 25, 12.5, and 6.25 µg/ml insulin of 1 µg/ml and DMEM groups showed absorbance value of
0.7669, 0.7253, 0.6563, 0.6481, 0.954, and 0.2653, respectively. It was found that there was a significant difference statistically in lipid droplets
accumulation among all groups (p<0.05) and tinocrisposide at a concentration of 50 µg/ml showed the highest lipid droplets accumulation in 3T3-L1
adipocyte cells.
Conclusion: From the study, it could be concluded that tinocrisposide was able to stimulate the differentiation of adipocyte cell and had
antihyperglycemic activity |
| format |
Article |
| author |
Adnan, Adek Zamrud Bakhtiar, Muhammad Taher ,, Annisa Fauzana Afriani, Tika Roesma, Dewi Imelda Putra, Andani Eka |
| author_facet |
Adnan, Adek Zamrud Bakhtiar, Muhammad Taher ,, Annisa Fauzana Afriani, Tika Roesma, Dewi Imelda Putra, Andani Eka |
| author_sort |
Adnan, Adek Zamrud |
| title |
Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation |
| title_short |
Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation |
| title_full |
Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation |
| title_fullStr |
Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation |
| title_full_unstemmed |
Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation |
| title_sort |
antihyperglycemic activity of tinocrisposide by stimulating 3t3-l1 adipocyte cell differentiation |
| publisher |
Asian Journal of Pharmaceutical and Clinical Research |
| publishDate |
2018 |
| url |
http://irep.iium.edu.my/70038/ http://irep.iium.edu.my/70038/ http://irep.iium.edu.my/70038/ http://irep.iium.edu.my/70038/1/70038_Antihyperglycemic%20activity%20of%20tinocrisposide%20by%20stimulating_article.pdf http://irep.iium.edu.my/70038/2/70038_Antihyperglycemic%20activity%20of%20tinocrisposide%20by%20stimulating_scopus.pdf |
| first_indexed |
2023-09-18T21:39:26Z |
| last_indexed |
2023-09-18T21:39:26Z |
| _version_ |
1777413040383197184 |