Antihyperglycemic activity of tinocrisposide by stimulating 3t3-L1 adipocyte cell differentiation
Objective: The aim of this study is to investigate the antihyperglycemic activity of tinocrisposide by stimulating 3T3-L1 adipocyte cell differentiation. Tinocrisposide is a furanoditerpene glycoside that was isolated from Tinospora crispa (Menispermaceae). Methods: Adipocyte cell differentiation...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Asian Journal of Pharmaceutical and Clinical Research
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70038/ http://irep.iium.edu.my/70038/ http://irep.iium.edu.my/70038/ http://irep.iium.edu.my/70038/1/70038_Antihyperglycemic%20activity%20of%20tinocrisposide%20by%20stimulating_article.pdf http://irep.iium.edu.my/70038/2/70038_Antihyperglycemic%20activity%20of%20tinocrisposide%20by%20stimulating_scopus.pdf |
| Summary: | Objective: The aim of this study is to investigate the antihyperglycemic activity of tinocrisposide by stimulating 3T3-L1 adipocyte cell differentiation.
Tinocrisposide is a furanoditerpene glycoside that was isolated from Tinospora crispa (Menispermaceae).
Methods: Adipocyte cell differentiation activity of tinocrisposide in interval concentrations of 50, 25, 12.5, and 6.25 µg/ml has been investigated
on 3T3-L1 cell line using insulin of 1 µg/ml as a positive and Dulbecco’s modified Eagle media (DMEM) as a negative control group. The effect of
tinocrisposide was quantified with oil red O staining method by measuring an absorbance of lipid solution in isopropanol at a wavelength (λ) of
520 nm.
Results: Tinocrisposide in the concentrations of 50, 25, 12.5, and 6.25 µg/ml insulin of 1 µg/ml and DMEM groups showed absorbance value of
0.7669, 0.7253, 0.6563, 0.6481, 0.954, and 0.2653, respectively. It was found that there was a significant difference statistically in lipid droplets
accumulation among all groups (p<0.05) and tinocrisposide at a concentration of 50 µg/ml showed the highest lipid droplets accumulation in 3T3-L1
adipocyte cells.
Conclusion: From the study, it could be concluded that tinocrisposide was able to stimulate the differentiation of adipocyte cell and had
antihyperglycemic activity |
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